磁性奈米顆粒於交變磁場加熱以及溫水熱療對血液抗發炎效應探討

Abstract

奈米磁顆粒在生物醫學研究領域中具有獨特的特性和應用潛力，其中磁性奈米顆粒已成為各種生物醫學應用的有前途的候選材料，包括靶向藥物傳遞、磁性熱療和磁共振成像（MRI）等方面。 在本研究中，我們專注於探討奈米磁顆粒對於加熱人體血液的抗發炎效應。我們使用了化學共沉法生成Fe3O4磁性奈米顆粒並進行表面修飾，接著使用紅外線熱影像儀觀察它們在高頻交流磁場下隨時間變化的升溫曲線。此外，為了確定其在人體血液中應用的安全性，我們還研究了磁性奈米顆粒與人類紅血球的生物相容性，即透過血漿凝固測試及紅血球溶解測定來檢驗。 在進行奈米磁顆粒血液抗發炎實驗的同時，我們亦透過水浴槽模擬了溫泉加熱的方式來對人體血液進行加熱，觀察其對血液中發炎因子的變化。 本研究的結果顯示，對於TNF-α和IL-6而言，不論HT或是TC-HT，奈米磁顆粒的微觀加熱和水浴槽模擬溫泉加熱的兩種方式皆能有效降低這兩種發炎因子的產生。然而， IL-1β的結果則證實了發炎終止反應會被奈米磁顆粒破壞。且只有HT模式下水浴槽模擬溫泉加熱的效果對於抗發炎仍然有用。

Nanomagnetic particles have unique properties and application potential in the Biomedical science, making them promising candidates for various biomedical applications, including Magnetic drug delivery, magnetic hyperthermia, and magnetic resonance imaging (MRI). In this study, we focused on exploring the anti-inflammatory effects of nanomagnetic particles when heating human blood. We synthesized Fe3O4 magnetic nanoparticles by using the chemical co-precipitation method and performed surface modifications. Infrared thermal imaging was employed to observe heating curves under alternating electromagnetic field. Additionally, to determine their safety in human blood applications, we investigated the biocompatibility of magnetic nanoparticles with human red blood cells through plasma coagulation tests and hemolysis assays. While conducting the anti-inflammatory experiments on blood with nanomagnetic particles, we also simulated hot spring heating using a water bath to heat human blood, observing changes in inflammatory factors. The results of this study showed that for TNF-α and IL-6, both HT and TC-HT modes of micro-heating by nanomagnetic particles and simulated hot spring heating using a water bath effectively reduced the production of these inflammatory factors. However, the results for IL-1β confirmed that its inflammation termination response was disrupted by nanomagnetic particles. Only the simulated hot spring heating in HT mode remained effective for anti-inflammation.