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Title: | 合成路易士X二聚體與KH-1抗原分子 Synthesis of dimeric LeX and KH-1 antigens |
Authors: | Bin-Ling Tsai 蔡秉霖 |
Advisor: | 翁啟惠(Chi-Huey Wong) |
Keyword: | 醣合成,一鍋化,腫瘤抗原,路易士X二聚體,KH-1, carbohydrate synthesis,one-pot,cancer antigen,dimer Lex,KH-1, |
Publication Year : | 2009 |
Degree: | 碩士 |
Abstract: | 醣類是組成細胞的四種基本成分之一,含量豐富且以多樣化形式存在於生物高分子中。癌細胞與正常細胞相比較下,在某些醣分子上的結構或量的層級上有不同表現。醣蛋白和醣脂質在醣化過程中,分子的不正常改變常會造成腫瘤的惡化。
路易士X二聚體與KH-1為大腸癌的細胞膜上顯著表現的兩種抗原分子,這兩種抗原分子大量的表現也與癌症的惡化前兆有關。所以路易士X二聚體與KH-1抗原分子可以用來發展疫苗或是用於醣晶片上作癌症的診斷。然而在鑑定和純化腫瘤細胞上的醣類抗原分子是非常困難,因此有機合成在醣生物學的研究上扮演重要的角色。在此篇論文中成功利用本實驗室所發展的一鍋化合成法來合成出三醣的路易士X、六醣的路易士X二聚體與七醣KH-1腫瘤抗原分子的類似物。 Among four major classes of macro-biomolecules-nucleic acids, proteins, lipids, and carbohydrates-carbohydrates are the least studied and understood.Carbohydrates and their conjugates are abundantly expressed on the out-surfaces of prokaryotic and eukaryotic cells and involved in various biological event. Especially, the surface of cancer cells frequently display glycans at different levels or with fundamentally different structures in comparison with that of normal cells, including the progression of tumor cell metastasis. Dimeric Lewis X and KH-1 are two tumor-associated antigens. These antigens are considered as the marker for malignancy and pre-malignancies involving colonic adenocarcinoma. Lewis X dimmer and KH-1 tumor associated antigens can be used to develop carbohydrate-based anticancer vaccines and carbohydrate microarray in diagnosis. However, the structure identification and isolation of homogeneous carbohydrate-based tumor antigen is very difficult, organic synthesis play an important role in glycobiology research. Finally, one-pot strategy was successfully developed to synthesis Lewis X monomer, Lewis X dimer hexasaccharide and KH-1 heptasaccharide analogues. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9436 |
Fulltext Rights: | 同意授權(全球公開) |
Appears in Collections: | 化學系 |
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ntu-98-1.pdf | 1.83 MB | Adobe PDF | View/Open |
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