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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 羅翊禎 | zh_TW |
| dc.contributor.advisor | Yi-Chen Lo | en |
| dc.contributor.author | 黃筱婷 | zh_TW |
| dc.contributor.author | Hsiao-Ting Huang | en |
| dc.date.accessioned | 2024-08-08T16:27:01Z | - |
| dc.date.available | 2024-08-09 | - |
| dc.date.copyright | 2024-08-08 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-08-04 | - |
| dc.identifier.citation | 李奕慧, 碳源對Pantoea vagans M17表面移行及生物膜形成的影響. 國立台灣大學生物資源暨農學院食品科技研究所碩士論文. 台北, 台灣, 2023.
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Appl Environ Microbiol 2019, 85 (22). Zhu, B.; Lou, M. M.; Xie, G. L.; Wang, G. F.; Zhou, Q.; Wang, F.; Fang, Y.; Su, T.; Li, B.; Duan, Y. P. Enterobacter mori sp. nov., associated with bacterial wilt on Morus alba L. Int J Syst Evol Microbiol 2011, 61 (Pt 11), 2769-2774. Zhu, B.; Zhang, G. Q.; Lou, M. M.; Tian, W. X.; Li, B.; Zhou, X. P.; Wang, G. F.; Liu, H.; Xie, G. L.; Jin, G. L., Genome sequence of the Enterobacter mori type strain, LMG 25706, a pathogenic bacterium of Morus alba L. J Bacteriol 2011, 193 (14), 3670-1. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/93831 | - |
| dc.description.abstract | 細菌的運動性包括泳動和表面移行能力，對宿主定殖和環境適應至關重要。與泳動能力相關的趨化性推動細菌向營養源移動或遠離有害物質。表面移行的細胞則透過細菌群的聚集，逐漸轉變為生物膜狀態。若食物成分是引誘劑，將導致細菌趨化性增加，從而使細菌積累，這可能會導致食物腐敗。我們先前的研究自截切小黃瓜中分離的Enterobacter mori FS08具顯著的泳動能力，並且小黃瓜汁對這株菌是很強的引誘劑。然而，此菌株與小黃瓜之間的關聯性尚未建立。本研究旨在 (1) 確認E. mori FS08是否促進小黃瓜腐敗；(2) 探討小黃瓜中的有機酸成分對E. mori FS08運動性相關表型的影響。實驗結果發現直接將E. mori FS08接種至新鮮截切小黃瓜上會導致外觀變質，並且消耗小黃瓜中的醣類成分。菌株特性分析中，觀察到有機酸在相同pH值下，並不會影響E. mori FS08的活菌數。值得注意的是，L-蘋果酸增強了泳動能力和正向趨化反應，但減少生物膜形成。反之，檸檬酸與DL-乳酸抑制了泳動和表面移行能力，且檸檬酸的抑制更為顯著，但這兩種有機酸皆促進了生物膜的形成。進一步的實驗證實添加L-蘋果酸使運動性相關基因 (fliC、flgM與motA) 表現量提升，添加檸檬酸則無顯著變化。而經過L-蘋果酸處理的轉錄組分析結果，鑑定出與運動性和生物膜形成相關的差異表達基因，其中fis (促進運動性的轉錄調節因子)、ahpC (氧化壓力防禦基因，其解毒作用可減少生物膜形成)、narK (硝酸鹽轉運蛋白，硝酸鹽會抑制生物膜形成) 表現量上升。另外，調控生物膜形成的群體感應相關基因亦受到抑制。這些實驗結果證明小黃瓜中的有機酸成分會調控訊息途徑影響細菌行為，可作為新鮮截切小黃瓜的預防措施和控制方法的重要參考。 | zh_TW |
| dc.description.abstract | Bacterial motility, encompassing swimming and swarming motility, is pivotal for host colonization and environmental adaptation. Chemotaxis, associated with swimming motility, propels bacteria toward nutrient sources or away from harmful substances. Swarming cells often aggregate and progressively transit to biofilm forming status. If the food factors are attractants, it will cause the increase of chemotaxis in bacteria, which results in bacteria accumulation. This may potentially lead to food spoilage. Our previous study isolated Enterobacter mori FS08 from fresh-cut cucumbers, exhibits pronounced swimming motility; however, the association between this strain and cucumber has not yet been established. The current study aimed to 1) investigate if E. mori FS08 leads to the spoilage of cucumber; 2) explore the impact of organic acids in cucumber on motility-related phenomena. The results demonstrate that E. mori FS08 caused cucumber spoilage by directly inoculating the strain onto fresh-cut cucumbers. The strain characterization indicated that organic acids did not affect the viable bacterial count of E. mori FS08 by the pH levels. Notably, L-malic acid enhanced swimming motility and positive chemotaxis response, but reduced biofilm formation. In contrast, citric acid and DL-lactic acid inhibited swimming and swarming motility, with citric acid having a more significant effect. However, both organic acids promoted biofilm formation. The subsequent analysis by qPCR found that the addition of L-malic acid led to the higher expression of motility-related genes (fliC, flgM, and motA), while the addition of citric acid showed no significant changes. Further transcriptomic analysis revealed that, in response to L-malic acid treatment, differentially expressed genes related to motility and biofilm formation were identified. The expression of fis (a transcriptional regulator promoting motility), ahpC (an oxidative stress defense gene whose detoxification activity can reduce biofilm formation), and narK (a nitrate transporter protein, with nitrate inhibiting biofilm formation) were upregulated. Additionally, quorum sensing-related genes that regulate biofilm formation were also downregulated. This study elucidated how organic acid components in cucumbers affect bacterial behavior and regulatory pathways, providing valuable insights into future preventative measures and control methods for fresh-cut cucumber handling. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-08-08T16:27:01Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-08-08T16:27:01Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員審定書 i
誌謝 ii 中文摘要 iii Abstract iv Graphical abstract v 目次 vi 圖次 ix 表次 xi 附錄次 xii 第一章、前言 1 第二章、文獻回顧 2 第一節、截切蔬果的加工處理與食品安全 2 一、截切蔬果之食安問題-以小黃瓜為例 2 二、小黃瓜的主要成分 2 三、細菌汙染截切小黃瓜之途徑 3 四、截切小黃瓜之品質指標 3 第二節、腸桿菌屬 (Enterobacter spp.) 4 一、基本特性 4 二、引發的問題 4 第三節、細菌運動性 6 一、泳動能力 (Swimming motility) 6 二、表面移行能力 (Swarming motility) 7 第四節、細菌運動性相關之菌株特性 8 一、趨化性 (Chemotaxis) 8 二、生物膜 (Biofilm) 9 第五節、細菌運動性與生物膜形成之調控機制 11 一、c-di-GMP調節運動性和生物膜形成 11 二、群體感應 (Quorum Sensing, QS) 12 第三章、研究目的與架構 14 第四章、材料與方法 16 第一節、材料 16 一、樣品 16 二、實驗菌株 16 三、藥品 17 四、培養基及實驗溶液之配製 18 五、儀器與設備 26 六、套裝軟體 27 第二節、實驗方法 27 一、截切小黃瓜之接菌試驗 27 二、小黃瓜品質指標測定 28 三、菌株特性分析 31 四、基因表現量分析 (qPCR) 34 五、轉錄組分析 (RNA-sequencing, RNA-seq) 40 第五章、結果與討論 44 第一節、確認E. mori FS08與截切小黃瓜腐敗之相關性 44 一、截切小黃瓜之菌數變化 44 二、E. mori FS08使小黃瓜外觀腐敗 47 三、E. mori FS08使截切小黃瓜之醣類含量減少 49 四、E. mori FS08不影響小黃瓜之有機酸含量變化 53 五、E. mori FS08不影響小黃瓜之pH值與可滴定酸 56 第二節、小黃瓜含有的有機酸成分對菌株特性之影響 59 一、混合碳源培養基添加不同有機酸不影響菌株生長 59 二、提高有機酸濃度不影響E. mori FS08之生長 66 三、於30 °C時，L-蘋果酸促進E. mori FS08泳動能力、檸檬酸則抑制 69 四、有機酸對E. mori FS08的泳動能力呈濃度效應 73 五、僅L-蘋果酸對E. mori FS08具吸引之趨化反應 78 六、混合碳源添加L-蘋果酸對E. mori FS08之趨化反應具加乘效果 81 七、L-蘋果酸不影響表面移行能力，檸檬酸與DL-乳酸則抑制 84 八、L-蘋果酸使生物膜形成減少，檸檬酸與DL-乳酸則促進生物膜的形成 87 第三節、L-蘋果酸與檸檬酸對E. mori FS08運動性相關基因之影響 90 一、引子效率 90 二、L-蘋果酸使E. mori FS08運動性相關基因表現量上升 93 第四節、L-蘋果酸與檸檬酸對E. mori FS08轉錄組之影響 98 一、主成分分析 (Principal Component Analysis, PCA) 98 二、差異表達基因 (differentially expressed genes, DEGs) 100 三、L-蘋果酸和檸檬酸處理與對照組相比有許多調控相似的DEGs 100 四、L-蘋果酸使能量產生、促進運動性和抑制生物膜形成相關的基因上調 102 五、KEGG分析顯示L-蘋果酸使群體感應受到抑制 108 六、GSEA分析顯示檸檬酸使鞭毛組裝相關基因表現量下降 111 第六章、結論與展望 113 第七章、參考文獻 114 第八章、附錄 126 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 有機酸 | zh_TW |
| dc.subject | 新鮮截切小黃瓜 | zh_TW |
| dc.subject | 生物膜 | zh_TW |
| dc.subject | 趨化性 | zh_TW |
| dc.subject | 泳動 | zh_TW |
| dc.subject | swimming motility | en |
| dc.subject | chemotaxis | en |
| dc.subject | biofilm | en |
| dc.subject | organic acid | en |
| dc.subject | Fresh-cut cucumber | en |
| dc.title | 有機酸對Enterobacter mori FS08運動性及生物膜形成的影響 | zh_TW |
| dc.title | Effect of organic acids on motility and biofilm formation of Enterobacter mori FS08 | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 林旭陽;李月嘉;王如邦;林乃君 | zh_TW |
| dc.contributor.oralexamcommittee | Hsu-Yang Lin;Yue-Jia Lee;Reu-Ben Wang;Nai-Chun Lin | en |
| dc.subject.keyword | 新鮮截切小黃瓜,有機酸,泳動,趨化性,生物膜, | zh_TW |
| dc.subject.keyword | Fresh-cut cucumber,organic acid,swimming motility,chemotaxis,biofilm, | en |
| dc.relation.page | 159 | - |
| dc.identifier.doi | 10.6342/NTU202401515 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2024-08-07 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 食品科技研究所 | - |
| 顯示於系所單位： | 食品科技研究所 | |
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