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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 呂廷璋 | zh_TW |
| dc.contributor.advisor | Ting-Jang Lu | en |
| dc.contributor.author | 曾品萱 | zh_TW |
| dc.contributor.author | Pin-Hsuan Tseng | en |
| dc.date.accessioned | 2023-11-20T16:15:12Z | - |
| dc.date.available | 2023-11-21 | - |
| dc.date.copyright | 2023-11-20 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-10-25 | - |
| dc.identifier.citation | 李雅雯，利用液相層析串聯高解析質譜法建立人參皂苷及皂苷元分析平台。國立台灣大學食品科技研究所學位論文 2021。臺北，臺灣。
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Zhu, D.; Zhou, Q.; Li, H.; Li, S.; Dong, Z.; Li, D.; Zhang, W., Pharmacokinetic characteristics of steamed notoginseng by an efficient LC–MS/MS method for simultaneously quantifying twenty-three triterpenoids. J. Agric. Food Chem. 2018, 66, 8187-8198. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91171 | - |
| dc.description.abstract | 人參皂苷 (ginsenosides) 為包含人參屬 (Panax) 之多種植物具生理活性成分。人參皂苷具有多元結構，活性與結構息息相關，且成分受到植物來源與加工的改變。因此可鑑別其組成的分析平臺是重要的品質管制工作。本研究使用液相層析串聯高解析度軌道阱質譜儀 (ultra-high performance liquid chromatography coupled to (Orbitrap) high resolution mass spectrometry, UHPLC-HRMS) 建立鑑別79種皂苷與5種皂苷元之方法。高解析質譜分析平台藉由化合物層析性質、精確分子量、同位素分佈與分子斷裂模式可以鑑別出4種齊墩果烷酸型 (oleanolic acid type, OA)、7種奧克梯隆型 (ocotillol type, OT)、30種原人參三醇 (protopanaxatriol, PPT)、43種原人參二醇 (protopanaxadiol, PPD) 人參皂苷。其中包含6種乙醯化 (acetyl-) 、8種丙二醯化 (malonyl-)、1種丁烯醯化 (butenoyl-) 及1種辛烯醯化 (octenoyl-) 人參皂苷，還有8對鏡相異構物，分別是24(S)/(R)-Pseudoginsenoside F11、20(S)/(R)-notoginsenoside R2、20(S)/(R)-ginsenoside Rh1、20(S)/(R)-ginsenoside Rg2、20(S)/(R)-ginsenoside Rg3、20(S)/(R)-ginsenoside Rs3、20(S)/(R)-protopanaxatriol、20(S)/(R)-protopanaxadiol。使用此分析平台對各種類人參與其商業產物進行分析，結果顯示所分析之亞洲參、三七、西洋參、絞股藍之人參皂苷以PPT型及PPD型人參皂苷為主要型態，但刺五加只有OA型皂苷。紅參中低極性人參皂苷含量比例較高。經酵母菌 (Saccharomyces cerevisiae) 發酵的人參皂苷，ginsenoside Rd含量增加。將酵母菌破菌後發現有23種人參皂苷出現在細胞內。此分析平台可有效辨識人參皂苷及皂苷元指紋圖譜及應用於人參原料和相關產品進行品質管制及加工監控指標，還可以用來分析微生物對人參皂苷的轉換。 | zh_TW |
| dc.description.abstract | Ginsenosides are the major bioactive ingredients in ginseng. Ginsenosides have structure reldiversity. Composition and the potential pharmacological activities have altered by biotaic sources and processing. It is necessary to establish an analytical platform to identify their composition. In this study, an ultra-high performance liquid chromatography coupled to high resolution (Orbitrap) mass spectrometry (UHPLC-HRMS/MS) was used to establish a method for the identification of 79 saponins and 5 sapogenins. The high-resolution mass spectrometry platform can identify 4 oleanolic acid type (OA), 7 ocotillol type (OT), 30 protopanaxatriol type (PPT), and 43 protopanaxadiol type (PPD) saponins based on compound chromatographic properties, precise molecular weight, isotope distribution and molecular fragmentation patterns. There were 6 acetyl-ginsenosides, 8 malonyl-ginsenosides, 1 butenoyl- ginsenosides, 1 octenoyl-ginsenosides, and 8 pairs of isomers that can be identified. PPD and PPT type saponins were the major components in Panax ginseng, Panax notoginseng, P. quinquefolium, and Gymnostemma pentaphyllum. At the same time, Eleutherococcus senticosus only had OA type saponins. The relative content of low polarity ginsenosides in red ginsengs is higher than that in Panax ginseng. The content of ginsenoside Rd increased after fermentation by Saccharomyces cerevisiae. Moreover, there were 23 ginsenosides in the intracellular space. The analytical platform established can be an effective tool to obtain the fingerprint of ginsenosides and sapogenins for quality control of ginseng raw material and products and indices of process monitoring. It can also be used to analyze the bioconversion of ginsenosides by yeast. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-11-20T16:15:12Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-11-20T16:15:12Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 致謝 I
摘要 II Abstract III 目錄 IV 圖目錄 VII 表目錄 XIX 第一章、前言 1 第二章、文獻回顧 2 2.1人參皂苷 2 2.1.1人參皂苷的化學結構 2 2.1.2人參皂苷的合成 4 2.1.3人參皂苷結構與生理活性關聯性 5 2.1.4人參皂苷結構與吸收代謝速率關聯性 7 2.2人參皂苷的分布 7 2.2.1植物來源 7 2.2.2人參皂苷加工過程的轉變 8 2.2.3人參皂苷生物轉換 9 2.3人參皂苷之吸收評估 10 2.4人參皂苷結構分析 12 2.4.1高效液相層析 12 2.4.2質譜結構分析 13 2.4.3核磁共振 14 第三章、研究目的及架構 16 第四章、材料與方法 17 4.1實驗材料 17 4.1.1植物樣品 17 4.1.2菌株 17 4.2實驗藥品 18 4.2.1標準品 18 4.2.2化學藥品 18 4.3實驗儀器及數據處理軟體 18 4.3.1前處理儀器設備 19 4.3.2超高效液相層析串聯質譜儀 19 4.3.3數據處理軟體 19 4.4實驗方法 20 4.4.1植物樣品前處理 20 4.4.2酸水解 20 4.4.3酵母菌對人參皂苷的轉換與吸收實驗 20 4.4.4層析儀的移動相配製與相關參數設定 21 4.4.5質譜儀參數設定 21 第五章、結果與討論 23 5.1人參皂苷皂苷分離條件優化 23 5.1.1人參皂苷以超高效能液相層析儀分離 23 5.1.2人參皂苷以高解析質譜儀解析結構 28 5.2人參皂苷在植物來源樣品中的分布 30 5.2.1人參皂苷在亞洲參、三七、西洋參、絞股藍及刺五加的分布 30 5.2.2人參皂苷在亞洲參葉、根、花與莖的分布 35 5.2.3人參皂苷在白參與紅參的分布 39 5.3酵母菌對人參皂苷的轉換與吸收 41 5.3.1酵母菌細胞外人參皂苷的轉換 41 5.3.2酵母菌能吸收的人參皂苷 45 第六章、結論 48 第七章、參考文獻 49 第八章、附錄 55 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 液相層析串聯高解析度質譜法 | zh_TW |
| dc.subject | 生物轉換 | zh_TW |
| dc.subject | 人參皂苷 | zh_TW |
| dc.subject | 三萜類皂素 | zh_TW |
| dc.subject | 譜型分析 | zh_TW |
| dc.subject | 液相層析串聯高解析度質譜法 | zh_TW |
| dc.subject | 三萜類皂素 | zh_TW |
| dc.subject | 人參皂苷 | zh_TW |
| dc.subject | 生物轉換 | zh_TW |
| dc.subject | 譜型分析 | zh_TW |
| dc.subject | bioconversion | en |
| dc.subject | Ginsenoside | en |
| dc.subject | triterpeneoid saponin | en |
| dc.subject | UHPLC-HRMS/MS | en |
| dc.subject | profiling platform | en |
| dc.subject | bioconversion | en |
| dc.subject | Ginsenoside | en |
| dc.subject | triterpeneoid saponin | en |
| dc.subject | UHPLC-HRMS/MS | en |
| dc.subject | profiling platform | en |
| dc.title | 人參皂苷譜型分析平台與其生物轉換應用 | zh_TW |
| dc.title | Profiling platform for ginsenosides and its bioconversion application | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.coadvisor | 陳宏彰 | zh_TW |
| dc.contributor.coadvisor | Hong-Jhang Chen | en |
| dc.contributor.oralexamcommittee | 羅翊禎;方銘志;張永和;王惠珠 | zh_TW |
| dc.contributor.oralexamcommittee | Yi-Chen Lo;Ming-Chih Fang;Yung-Ho Chang;Huei-Ju Wang | en |
| dc.subject.keyword | 人參皂苷,三萜類皂素,液相層析串聯高解析度質譜法,譜型分析,生物轉換, | zh_TW |
| dc.subject.keyword | Ginsenoside,triterpeneoid saponin,UHPLC-HRMS/MS,profiling platform,bioconversion, | en |
| dc.relation.page | 208 | - |
| dc.identifier.doi | 10.6342/NTU202304205 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2023-10-26 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 食品科技研究所 | - |
| dc.date.embargo-lift | 2028-10-25 | - |
| 顯示於系所單位： | 食品科技研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-112-1.pdf 未授權公開取用 | 23.63 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。