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標題: | 施用液化澱粉芽胞桿菌對葉萵苣淹水耐受性之影響 Effects of Application of Bacillus amyloliquefaciens on Submergence Tolerance of Leaf Lettuce |
作者: | 林妤芊 Yu-Chien Lin |
指導教授: | 許富鈞 Fu-Chiun Hsu |
關鍵字: | 萵苣,淹水逆境,植物根際促生菌,活性氧物質,抗氧化酵素, lettuce,submergence stress,plant growth promoting rhizobacteria,reactive oxygen species,antioxidant enzymes, |
出版年 : | 2023 |
學位: | 碩士 |
摘要: | 台灣每年均有因水患導致的農產損失,在氣候變遷的影響下,極端降水更是無法避免。了解淹水逆境對於植株造成的危害、知道植株如何因應淹水逆境,以及如何提升植株淹水耐受性是需要研究的課題。長時間淹水下,葉萵苣(Lactuca sativa L.)‘鹿角’於復氧期間性狀觀察的結果,諸如之相片紀錄、葉片評分、葉數變化、株高變化、鮮重及乾重記錄,皆差於‘興農二號’,顯示‘鹿角’為淹水耐受性較低之品種。然而於換盆時施用植物根際促生菌,液化澱粉芽胞桿菌(Bacillus amyloliquefaciens)可提升‘鹿角’復氧期間之性狀表現。‘鹿角’未施用植物根際促生菌之對照組於復氧期超氧陰離子有累積情形,然而施用菌液的組別較無累積。於基因表現量上,澆灌菌液組別之抗壞血酸過氧化物酶(ascorbate peroxidase, APX)及穀胱甘肽過氧化物酶(glutathione peroxidase, POX)於復氧期皆顯著高於對照組,顯示植物根際促生菌對植株抗氧化酵素之影響。上述結果顯示植物根際促生菌可能透過影響復氧期間抗氧化酵素基因表現量提升植株淹水耐受性。此結果對於了解植物根際促生菌影響植株淹水耐受性之機制有一定幫助。 Every year in Taiwan, agricultural production is lost due to floods. Under the influence of climate change, extreme precipitation is even more inevitable. Understanding the damage caused by submergence stress to plants, knowing how plants respond to submergence stress, and how to improve plant submergence tolerance are topics that need to be studied. The results of trait observation of leaf lettuce (Lactuca sativa L.) ‘Lujiao’ during reoxygenation under long-term submergence, such as photo records, leaf score, leaf number change, plant height change, fresh weight, and dry weight records, are all worse than that of ‘Xingnong No.2’, indicating that ‘Lujiao’ is a variety with low tolerance to submergence. However, the application of plant growth promoting rhizobacteria, Bacillus amyloliquefaciens, during repotting can improve the performance of ‘Lujiao’ during reoxygenation. The control group of ‘Lujiao’ without plant growth promoting rhizobacteria accumulated superoxide anions during the reoxygenation period, but the group treated with bacterial solution did not accumulate superoxide anions. In terms of gene expression, ascorbate peroxidase (APX) and glutathione peroxidase (POX) in the bacteria-treated group were significantly higher than those in the control group during the reoxygenation period, showing effects of plant growth promoting rhizobacteria on plant antioxidant enzymes. The above results indicated that the plant growth promoting rhizobacteria may enhance the submergence tolerance of plants by affecting the expression of antioxidant enzyme genes during reoxygenation. This result is helpful to understand the mechanism of plant growth promoting rhizobacteria affecting plant submergence tolerance. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/90676 |
DOI: | 10.6342/NTU202303340 |
全文授權: | 未授權 |
顯示於系所單位: | 園藝暨景觀學系 |
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