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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝淑貞 | zh_TW |
| dc.contributor.advisor | Shu-Chen Hsieh | en |
| dc.contributor.author | 郭詩筠 | zh_TW |
| dc.contributor.author | Shih-Yun Kuo | en |
| dc.date.accessioned | 2023-09-15T16:16:09Z | - |
| dc.date.available | 2023-09-16 | - |
| dc.date.copyright | 2023-09-15 | - |
| dc.date.issued | 2022 | - |
| dc.date.submitted | 2002-01-01 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/89689 | - |
| dc.description.abstract | 近年來全球爆發多起由病毒所引起的呼吸道傳染疾病，包含 SARS、MERS 以及現階段的 SARS-CoV-2，造成疫苗需求上升，推動了新型疫苗的發展。益生 菌疫苗是一種口服疫苗，可以引起全身性及黏膜免疫反應，產生特異性抗體 IgG 及 SIgA，在病毒入侵黏膜時即被抗體中和，阻止感染。此外，益生菌疫苗具有 低成本、容易儲存、方便接種等優勢，適合應用於大型流行性疾病的預防。然而， 受到嚴峻腸胃道環境以及口服耐受的挑戰，益生菌疫苗需要提高表達水平以及添 加佐劑來引發有效的保護反應。因此，本研究透過基因工程調整啟動子與抗原表 達方式，並設計 SARS-CoV-2 當作抗原，以乳酸菌作為載體發展高表達抗原之 益生菌疫苗平台。分別使用誘導型及連續表現型啟動子，建造錨定型、分泌型與 分泌錨定型抗原表達模式，後續還比較了質體的不同抗生素抗性基因對抗原表達 的影響。透過流式細胞儀偵側乳酸菌表面抗原量，發現在 ampicillin 抗性基因 (AmpR)篩選下整體表現比 erythromycin(ErmR) 好，且 AmpR 當中的連續型 啟動子(P11)比誘導型(sppA)有更高的表現量，又以錨定型表達模式為最高 者。同時藉由螢光顯微鏡確認抗原確實表現在乳酸菌表面。此外，在不同宿主中， 重 組 菌 株 Lactiplantibacillus plantarum PS128 表 現 量 比 Lactiplantibacillus plantarum K68 及 Latilactobacillus sakei 要來得好，因此，選擇 AmpR 連續型啟 動子的錨定表達質體 pA-P11-ASD，搭配 L.plantarumPS128 可作為高表達抗原 之 COVID-19 益生菌疫苗平台。 | zh_TW |
| dc.description.abstract | In recent years, there are many outbreaks of respiratory infectious diseases caused by viruses around the world, including SARS, MERS and the current SARS-CoV-2, resulting in rising demand for vaccines and thus promoting the development of different vaccines. Probiotic vaccine is an oral vaccine that can induce systemic and mucosal immune responses to produce specific antibodies IgG and SIgA, which neutralize viruses to prevent their mucosa invasion. In addition, probiotic vaccines are good choices for pandemic prevention due to the advantages of low cost, easy storage, and convenient vaccination. However, challenged by the harsh gastrointestinal environment and oral tolerance, probiotic vaccines require increased expression levels and the addition of adjuvants to elicit an effective protective response. Therefore, this study adjusted the promoter and antigen expression pattern through genetic engineering, using SARS-CoV-2 as the antigen and lactic acid bacteria as the carrier to develop a probiotic vaccine platform with high antigen expression. Inducible and constitutive promoters were used to construct anchored, secreted and secreted-anchored antigen expression patterns, and antibiotic resistance gene was subsequently replaced for antigen expression analysis. Flow cytometry was used to detect the surface antigen of lactic acid bacteria, and the results displayed that the overall performance of ampicillin resistance gene (AmpR) was better than that of erythromycin, and the constitutive promoter (P11) in AmpR was higher than the inducible type (sppA), furthermore, the anchored expression pattern exhibited the highest expression level. The similar result was revealed under fluorescence microscope, showing that the antigen was indeed expressed on the surface of lactic acid bacteria. Among different hosts, the antigen expression level of Lactiplantibacillus plantarum PS128 was better than that of Lactiplantibacillus plantarum K68 and Latilactobacillus sakei. Therefore, transforming pA-P11-ASD to L. plantarum PS128 could serve as a probiotic vaccine platform for future development of COVID vaccine. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-09-15T16:16:09Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-09-15T16:16:09Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 謝辭 I
摘要 II Abstract III 總目錄 V 圖目錄 IX 縮寫表 X 壹、文獻探討 1 一、益生菌疫苗 1 (一)益生菌疫苗的優勢 1 (二)益生菌疫苗免疫誘發機制 1 (三)益生菌疫苗的應用 2 二、乳酸菌 4 (一)Lactiplantibacillus plantarum PS128 4 (二)Lactiplantibacillus plantarum K68 5 (三)Latilactobacillus sakei 5 三、益生菌疫苗設計要點 6 (一)質體與宿主之間的穩定性 6 (二)優化抗原生產量 6 1. 抗原表達系統 6 2. 增強基因的表達-啟動子 9 3. 啟動子強度 11 4. 優化密碼子 11 (三)提高免疫激活效率 12 四、SARS-CoV-2 14 (一)病毒的特性 14 (二)感染途徑與臨床症狀 14 (三)Spike protein 15 貳、研究目的與實驗架構 17 一、研究目的 17 二、實驗架構 18 參、實驗材料與方法 19 一、菌株、引子、質體 19 二、藥品 23 三、儀器設備 26 四、實驗方法 28 (一)E.coli TOP10 Competent cell 製備 28 (二)克隆質體流程 29 1. Enzyme digestion 29 2. Ligation 30 3. Transformation 30 4. PCR screen 31 5. 洋菜凝膠電泳 31 (三)質體克隆統整 32 1. E. coli expression vector cloning 32 2. ErmR Plasmids 32 3. AmpR plasmid 33 (四)利用 PCR (polymerase chain reaction) 放大目標片段 35 1. PCR-pLp3050 35 2. PCR-AmpR 35 3. NdeI-ASD-HindIII 36 4. SalI-SD-HindIII 36 (五)使用 Coomassie blue 偵測 E. coli 誘導表現 37 (六)使用 Western blot 偵測 E. coli 誘導表現 38 (七)乳酸菌勝任細胞製備 40 (八)乳酸菌電穿孔轉形 40 (九)以流式細胞儀確認乳酸菌表面重組蛋白的表達 41 (十)以倒立式螢光顯微鏡確認乳酸菌表面重組蛋白的表達 42 (十一)篩選 Ampicillin 抗生素濃度 42 (十二)以西方墨點法確認乳酸菌表面重組蛋白的表達 42 肆、實驗結果 45 一、ASD 抗原表達的基因設計 45 二、利用 E. coli 表達目標抗原 SD 48 (一)建構 E. coli 表現型質體 48 (二)確認重組菌株 E. coli pRSETC-SD 之 SD 蛋白表現 51 三、分析 L. plantarum PS128 ErmR 質體 Spike 蛋白質表現 53 (一)ErmR 系列質體建構流程 53 (二)以流式細胞儀偵測 ErmR 重組菌株表面的抗原量 58 四、置換篩選基因 59 (一)AmpR 抗性基因的替換以及不同表現型的建造 59 (二)篩選 Ampicillin 抗生素濃度 67 五、偵測重組菌株表面抗原 68 (一)以流式細胞儀偵測 AmpR 組別之表面抗原量 68 (二)以螢光顯微鏡偵測重組菌株表面螢光 72 (三)重組菌株高表達抗原之時間點 75 六、挑選益生菌疫苗平台宿主 77 七、確認重組菌株蛋白的表達 78 八、Omicron 抗原序列設計 80 伍、討論 83 一、AmpR 與 ErmR 對乳酸菌在表達抗原時所帶來的影響 83 二、誘導系統 sppA 與 連續系統 P11 之探討 84 三、表現型之間的螢光表現討論 85 四、探討革蘭氏陽性菌細胞壁蛋白之萃取 87 五、未來展望 87 陸、結論 89 柒、參考文獻 90 | - |
| dc.language.iso | zh_TW | - |
| dc.title | 建立高表達抗原之益生菌疫苗平台 | zh_TW |
| dc.title | Development of the probiotic vaccine platform with highly expressed antigens | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 110-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 蔡英傑 | zh_TW |
| dc.contributor.oralexamcommittee | Ching-Chuan Kuo;Tsun-Yung Kuo;Ying-Chieh Tsai | en |
| dc.subject.keyword | 益生菌疫苗,LactiplantibacillusplantarumPS128,P11 啟動子,高表達,抗生素抗性基因, | zh_TW |
| dc.subject.keyword | probiotic vaccine,Lactiplantibacillus plantarum PS128,P11 promoter,high expression level,antibiotic resistance gene, | en |
| dc.relation.page | 98 | - |
| dc.identifier.doi | 10.6342/NTU202203847 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2022-09-27 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 食品科技研究所 | - |
| 顯示於系所單位： | 食品科技研究所 | |
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