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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭宗記 | zh_TW |
| dc.contributor.advisor | Tzong-Jih Cheng | en |
| dc.contributor.author | 蔡佩珈 | zh_TW |
| dc.contributor.author | Pei-Chia Tsai | en |
| dc.date.accessioned | 2023-09-07T16:43:21Z | - |
| dc.date.available | 2025-08-01 | - |
| dc.date.copyright | 2023-09-11 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-07-24 | - |
| dc.identifier.citation | 衛生福利部。2022。110年國人死因統計結果。台北：衛生福利部。網址：https://www.mohw.gov.tw/cp-16-70314-1.html。上網日期：2022-08-30。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/89369 | - |
| dc.description.abstract | 生物感測器一直以來透過靈敏度和高特異性快速地針對待測分子進行的檢測，在各個領域具有高度的應用價值。近年來，醫療檢驗體系中為了落實照護點的概念，儘管有許多高精度的技術和設備結合了半導體製程或是微奈米加工技術將檢測機構微小化帶來了便利性，卻帶來許多挑戰難以成為市售的產品，也因此提高了相對簡易且成本較低的檢測機構開發需求。本文將以低成本且可攜帶的電容生物感測器的開發為主旨，以研究電化學感測器為基礎探討常見的界面分子結合策略以及利用阻抗頻譜分析和電阻、電容、電感零件分析測試儀（LCR 錶）分析自組裝單層（SAM）絕緣度和蛋白修飾後的電容尺度，再以類比轉數位模組結合網印碳電極組成電子式的生物感測器透過電容變化實現分子感測之目的。本文以自製金電極取代市售柱狀金電極，提高分析效率，在製備好自組裝單層後將蛋白透過交聯劑固定在金電極，由阻抗量測確效，研究過程中電化學量測工具有不同分析的適性及絕緣性，阻抗過大會造成分析上的不準確性。最後選用實驗室開發的類比轉數位模組結合拋棄式網印碳膠電極，以聚丙烯酸樹脂作為感測界面絕緣層，再以硝化纖維素薄膜作為吸附層，將10 mg/ml為蛋白（抗原）固定化在感測界面進行抗體結合。目前在結果部分可以在電容值變化上確認生物辨識元件之步驟，透過雷射顯微鏡分析感測介面上的絕緣層與吸附層物理性狀，了解生辨識元件之製程效果，以電容值變化分析抗原固定以及抗體結合之成果，同時，利用雙辛可寧酸測定法（Bicinchonininc acid assay, BCA）和增強型化學發光試劑（Enhanced chemiluminescence, ECL）光呈色分別對抗原以及抗體進行吸附與結合效果的確效與電容變化結果比對，在不同濃度抗原吸附在感測界面後整體電容訊號變化倍率提升了約5倍（𝚫Capacitance/fold 值從0.01%提升至0.05%），最後在抗原抗體結合後可以看到電容下降的現象，在抗體濃度為0-25 𝜇g/ml具有分辨性，達到檢測之目的，日後若能調整訊大小或優化電極與或是感測界面的材料，進而將感測的敏感度提高、訊號放大，相信必能在分子檢測上提供另種選擇。 | zh_TW |
| dc.description.abstract | Biosensors have always been valuable in various fields due to their ability to quickly and accurately detect target molecules. Recently, there has been a movement to implement the Point-of-Care Testing concept in medical testing systems by combining high-precision technologies and equipment with semiconductor processes or micro-nano processing technologies to miniaturize detection mechanisms and improve convenience. However, this has created challenges in commercializing these technologies and thus increased the demand for simpler, more cost-effective detection mechanisms.
This thesis focuses on the development of a low-cost and portable capacitive biosensor. It explores common interface molecule binding strategies based on electrochemical sensors and analyzes the insulation and protein-modified capacitance degrees using impedance spectroscopy and LCR meter analysis of self-assembled monolayers (SAMs). A capacitive biosensor is then constructed using an analog-to-digital converter module combined with screen-printed carbon electrodes to achieve molecular sensing through changes in capacitance. Homemade gold electrodes are used instead of commercial cylindrical gold electrodes to improve analysis efficiency. After the SAM is prepared, the protein is fixed to the gold electrode via a crosslinker, and impedance measurements confirm the effectiveness of the process. During the study, it is found that the electrochemical measurement tool has different suitability and limitation for analysis, and excessive impedance can cause inaccuracies in analysis. To overcome this problem, a disposable screen-printed carbon electrode is combined with a laboratory-developed analog-to-digital converter module. A polyacrylic acid resin is used as the insulation layer, and a nitrocellulose film is used as the adsorption layer to immobilize the protein (antigen) at the sensing interface for antibody binding at a concentration of 10 mg/ml. This study has confirmed that changes in capacitance value can be used to confirm the steps of the biometric recognition component. By analyzing the physical properties of the insulation layer and adsorption layer on the sensing interface through laser microscopy, the processing effect of the biometric recognition component can be understood, and capacitance value changes can be analyzed for the results of antigen fixation and antibody binding. Additionally, the Bicinchoninic Acid (BCA) Assay and Enhanced chemiluminescence (ECL) cold light colorimetry were used to verify the adsorption and binding effects of antigens and antibodies, and were compared to the capacitance change results. Different concentrations of antigens were applied to the sensing interface resulting in an overall increase in the capacitance signal ratio of approximately 5 times (the ΔCapacitance/fold value increased from 0.01% to 0.05%). The antigen-antibody binding led to a decrease in capacitance, providing a resolution for antibody concentrations of 0-25 𝜇g/ml and meeting the detection objectives. In the future, amplifying the signal and increasing the sensor's sensitivity can be achieved through signal size adjustment or optimization of the electrode and sensing interface materials, providing an alternative choice for molecular detection. | en |
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| dc.description.provenance | Made available in DSpace on 2023-09-07T16:43:21Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 致謝 I
摘要 II ABSTRACT IV 目錄 VI 圖目錄 IX 表目錄 XII 前言 1 1.1 研究背景 1 1.2 研究目的 3 1.3 研究架構 5 第二章 文獻回顧 6 2.1生物標記物 6 2.2 生物感測器 8 2.2.1電化學生物感測器 8 2.2.2 EIS-based生物感測器 10 2.2.3電化學生物感測器之電容式感測方法 12 2.2.4電化學感測器的感測界面設計 13 2.2.5 生化分析工具中的分子親和材料 15 2.3電容式生物感測器的發展 17 第三章 研究方法 19 3.1 實驗藥品與材料 19 3.2 實驗儀器 19 3.3電化學感測器製程 20 3.3.1 CD金電極的製備 20 3.3.2 蛋白於金電極之化學性結合方法 20 3.4 電化學感測系統有效性之驗證 21 3.4.1 電化學阻抗圖譜法（EIS）及衍伸之電化學電容圖譜法ECS 21 3.4.2 CD金電極表面電化學分析與感測分析 22 3.4.3 CD金電極連接電阻、電容、電桿零件測試儀的電容量測 23 3.5電子式電容感測器結合網印碳電極感測蛋白分子 24 3.5.1 電子式電容感測架構 24 3.5.2抗原的固定化程序 25 3.6抗原吸附效果及抗體抗原專一性結合的生化試驗確效 26 3.6.1 雙辛可寧酸測定法蛋白與銅離子反應實驗 26 3.6.2 ECL冷光呈色對抗體結合的確效 26 3.6.3 雷射顯微鏡分析生物辨識元件之薄膜厚度 26 第四章 結果與討論 28 4.1以電化學阻抗圖譜法（EIS）測定蛋白 28 4.1.1以柱狀金電極/自製CD金電極確認蛋白固定之方法 28 4.1.2自製CD金電極之電化學特性與蛋白固定化參數 33 4.1.3蛋白固定於自製辨識元件之電化學阻抗定量分析 39 4.1.4 ECS對於蛋白定量可行性頻估 43 4.2以EIS波德圖及LCR錶測定固定於SAM上的蛋白 45 4.2.1 EIS波德圖分析 45 4.2.2 LCR錶進行電容分析 48 4.3電容式ANTI-BSA生物感測器 53 4.3.1電容感測模組性能 53 4.3.2絕緣層與吸附層的建構與特性 55 4.3.3硝化纖維素膜吸附層性能 62 第五章 結論與未來展望 73 參考文獻 75 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 自組裝單層 | zh_TW |
| dc.subject | 電容式生物感測器 | zh_TW |
| dc.subject | 硝化纖維素 | zh_TW |
| dc.subject | 生物標記物 | zh_TW |
| dc.subject | 電化學阻抗頻譜分析 | zh_TW |
| dc.subject | Nitrocellulose | en |
| dc.subject | Self-assembled monolayers | en |
| dc.subject | Biomarkers | en |
| dc.subject | Electrochemical impedance spectroscopy | en |
| dc.subject | Capacitive biosensor | en |
| dc.title | 用於偵測生物標記物之電容式生物感測器開發 | zh_TW |
| dc.title | Capacitive Biosensor for Detecting Protein Biomarkers | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 陳林祈;康一龍;吳靖宙;李博仁 | zh_TW |
| dc.contributor.oralexamcommittee | Lin-Chi Chen;Yit-Lung Khung ;Ching-Chou Wu;Bor-Ran Li | en |
| dc.subject.keyword | 電容式生物感測器,硝化纖維素,生物標記物,自組裝單層,電化學阻抗頻譜分析, | zh_TW |
| dc.subject.keyword | Capacitive biosensor,Nitrocellulose,Biomarkers,Self-assembled monolayers,Electrochemical impedance spectroscopy, | en |
| dc.relation.page | 84 | - |
| dc.identifier.doi | 10.6342/NTU202301818 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2023-07-25 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 生物機電工程學系 | - |
| dc.date.embargo-lift | 2025-08-01 | - |
| 顯示於系所單位： | 生物機電工程學系 | |
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| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-111-2.pdf | 5.17 MB | Adobe PDF | 檢視/開啟 |
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