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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 徐善慧 | zh_TW |
dc.contributor.advisor | Shan-hui Hsu | en |
dc.contributor.author | 黃于真 | zh_TW |
dc.contributor.author | Yu-Chen Huang | en |
dc.date.accessioned | 2023-02-01T17:02:09Z | - |
dc.date.available | 2023-11-14 | - |
dc.date.copyright | 2023-02-01 | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2023-01-12 | - |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/83243 | - |
dc.description.abstract | 開發可編程形變的水凝膠系統致動器是一個非常重要的議題,並已引起基礎和應用研究的高度關注。大部分的致動器是不可降解或不能在生理環境下運行的。在此,探索具熱響應和可生物降解的甲基丙烯醯基明膠-聚異丙基丙烯醯胺水凝膠 (即GN水凝膠) 網絡作為雙層水凝膠的主動層。以小角度X光散射顯示GN水凝膠在溫度導致之相變時形成中球體結構 (約230 Å),並由流變數據支持GN水凝膠有三維 (3D) 列印性和可調控的機械性質。通過改變層厚度來優化由主動GN和被動GelMA層組成的雙層水凝膠致動器,以實現曲率約為5.5 cm-1的大、異向性和可重複的彎曲。在塊狀製造的過程中,不同圖案的主動層被設計用於可編程控制的驅動。3D列印的GN水凝膠結構根據其結構設計減少約25-60%的體積,並由37 °C的熱觸發驅動得到更好的解析度,當置於室溫下能完全再次溶脹。更複雜的3D列印蝴蝶致動器展示了通過熱響應來模擬翅膀動作的能力。此外,GN水凝膠中的成肌細胞在14天內表現出約376%的顯著增殖。這項研究為開發用於生物醫學應用的仿生設備、軟機器人和人造肌肉提供一種新的製造策略。 | zh_TW |
dc.description.abstract | Development of hydrogel-based actuators with programmable deformation is an important issue that arouses high attention in fundamental and applied research. Most of these actuators are non-biodegradable or work under non-physiological conditions. Herein, a thermoresponsive and biodegradable gelatin methacryloyl (GelMA)-poly(N-isopropyl acrylamide) hydrogel (i.e., GN hydrogel) network was explored as the active layer of a bilayer actuator. Small-angle X-ray scattering (SAXS) showed that GN hydrogel formed mesoglobular structures (~ 230 Å) upon thermally induced phase transition. Rheological data supported that GN hydrogel had 3D printability and tunable mechanical properties. A bilayer hydrogel actuator composed of active GN and passive GelMA layers was optimized by varying the layer thickness and compositions to achieve large, anisotropic, and reproducible deformation with a curvature ~ 5.5 cm-1. Different active layer patterns of the bilayer hydrogel were designed for actuation in programmable control. The 3D printed GN hydrogel structures reduced ~ 25-60% volume depending on the structure design and gained better resolution at 37 °C due to thermo-triggered actuation, while were able to fully reswell at room temperature. A more intricate 3D printed butterfly actuator displayed the ability to mimic the wing movement through thermoresponsiveness. In addition, myoblasts laden in GN hydrogel exhibited significant proliferation of ~ 376% in 14 days. This study provides a new fabrication strategy for developing soft robotics, biomimetic devices, and artificial muscles for biomedical applications. | en |
dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-02-01T17:02:09Z No. of bitstreams: 0 | en |
dc.description.provenance | Made available in DSpace on 2023-02-01T17:02:09Z (GMT). No. of bitstreams: 0 | en |
dc.description.tableofcontents | 致謝 I
摘要 II Abstract III 目錄 V 圖目錄 IX 表目錄 XI 第一章 文獻回顧 1 1.1. 水凝膠 1 1.2. 響應性水凝膠致動器 1 1.3. 同向性與異向性水凝膠 1 1.4. 提升三維列印解析度之方式 2 1.5. 研究目的 2 第二章 研究方法 4 2.1. 研究架構 4 2.2. 甲基丙烯醯基明膠之合成 6 2.3. 甲基丙烯醯基明膠-聚異丙基丙烯醯胺水凝膠合成與表徵 7 2.3.1. 合成甲基丙烯醯基明膠-聚異丙基丙烯醯胺水凝膠 7 2.3.2. 傅立葉轉換紅外光譜儀 (Fourier Transform Infrared Spectrometer, FT-IR) 7 2.3.3. 交聯度測定 8 2.3.4. 差示掃描量熱儀 (Differential scanning calorimetry, DSC) 8 2.3.5. 掃描式電子顯微鏡 (Scanning Electron Microscope, SEM) 8 2.3.6. 隨溫度變化之溶脹比及含水量 9 2.3.7. 小角度X光散射 (Small-angle X-ray Scattering, SAXS) 分析 9 2.4. 溫度響應性雙層水凝膠之製備與表徵 9 2.4.1. 製備具溫度響應之雙層水凝膠 10 2.4.2. 雙層水凝膠最佳化 10 2.4.3. 雙層水凝膠之掃描式電子顯微鏡 (SEM) 觀察 11 2.5. 致動展示實驗 11 2.6. GN水凝膠的流變性質 12 2.7. 3D列印具溫度響應性水凝膠之構造 13 2.8. 3D列印溫度響應性水凝膠致動器 13 2.9. GN與GelMA水凝膠之體外降解 14 2.10. 細胞實驗 15 2.10.1. 細胞培養 15 2.10.2. 細胞於GN水凝膠中之短期存活率 15 2.10.3. 細胞於GN水凝膠中之增殖分析 15 2.11. 統計學分析 16 第三章 實驗結果 17 3.1. GN和GelMA材料與水凝膠之表徵 17 3.1.1. 核磁共振儀分析 (Nuclear Magnetic Resonance, NMR) 17 3.1.2. GN水凝膠之組別與交聯度 17 3.1.3. 傅立葉轉換紅外光譜儀 (Fourier Transform Infrared Spectrometer, FT-IR) 18 3.1.4. 差示掃描量熱儀 (DSC)、溶脹率及含水量分析 19 3.1.5. 小角度X光散射 (SAXS) 分析 23 3.2. 溫度響應性雙層水凝膠 26 3.2.1. 雙層水凝膠之被動層最佳化 26 3.2.2. 雙層水凝膠之厚度最佳化 28 3.2.3. 雙層水凝膠之可逆性測試 29 3.2.4. 雙層水凝膠之掃描式電子顯微鏡 (SEM) 及巨觀照片觀察 30 3.3. 雙層水凝膠致動 32 3.4. GN水凝膠的流變性質 34 3.5. 3D列印GN水凝膠結構之溫度響應行為 37 3.6. 3D列印雙層水凝膠致動器之溫度響應行為 39 3.7. GN及GelMA水凝膠之體外降解 42 3.8. GN水凝膠之短期細胞毒性 43 3.9. GN及GelMA水凝膠之細胞增殖 43 第四章 討論 45 第五章 結論 52 參考文獻 53 | - |
dc.language.iso | zh_TW | - |
dc.title | 具3D列印性之熱響應甲基丙烯醯基明膠-聚異丙基丙烯醯胺水凝膠之仿生雙層水凝膠致動器研究 | zh_TW |
dc.title | A Biomimetic Bilayer Hydrogel Actuator Based on Thermoresponsive GelMA-PNIPAM Hydrogel with 3D Printability | en |
dc.title.alternative | A Biomimetic Bilayer Hydrogel Actuator Based on Thermoresponsive GelMA-PNIPAM Hydrogel with 3D Printability | - |
dc.type | Thesis | - |
dc.date.schoolyear | 111-1 | - |
dc.description.degree | 碩士 | - |
dc.contributor.oralexamcommittee | 鄭有舜;張書瑋;周佳靚 | zh_TW |
dc.contributor.oralexamcommittee | U-Ser Jeng;Shu-Wei Chang;Chia-Ching Chou | en |
dc.subject.keyword | 致動器,3D列印,熱響應,雙層水凝膠,異丙基丙烯醯胺, | zh_TW |
dc.subject.keyword | actuator,3D printing,thermoresponsive,bilayer hydrogel,N-isopropyl acrylamide, | en |
dc.relation.page | 57 | - |
dc.identifier.doi | 10.6342/NTU202300060 | - |
dc.rights.note | 未授權 | - |
dc.date.accepted | 2023-01-12 | - |
dc.contributor.author-college | 工學院 | - |
dc.contributor.author-dept | 高分子科學與工程學研究所 | - |
顯示於系所單位: | 高分子科學與工程學研究所 |
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