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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李昆達(Kung-Ta Lee) | |
| dc.contributor.author | Meng-Ting Lee | en |
| dc.contributor.author | 李孟庭 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:09:50Z | - |
| dc.date.available | 2021-09-02 | |
| dc.date.available | 2022-11-23T09:09:50Z | - |
| dc.date.copyright | 2021-09-02 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-08-19 | |
| dc.identifier.citation | Ajithkumar, D., Seeni, S. (1998). Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree. Plant Cell Rep., 17(5), 422-426. Asano, T., Kobayashi, K., Kashihara, E., Sudo, H., Sasaki, R., Iijima, Y., . . . Yamazaki, M. (2013). Suppression of camptothecin biosynthetic genes results in metabolic modification of secondary products in hairy roots of Ophiorrhiza pumila. Phytochemistry, 91, 128-139. Baek, S., Ho, T.-T., Lee, H., Jung, G., Kim, Y. E., Jeong, C.-S., Park, S.-Y. (2020). Enhanced biosynthesis of triterpenoids in Centella asiatica hairy root culture by precursor feeding and elicitation. Plant Biotechnol. Rep., 14, 45-53. Baño, M. J., Lorente, J., Castillo, J., Benavente-García, O., Río, J. A., Ortuño, A., . . . Gerard, D. (2003). Phenolic Diterpenes, Flavones, and Rosmarinic Acid Distribution during the Development of Leaves, Flowers, Stems, and Roots of Rosmarinus officinalis. Antioxidant Activity. J. Agric. Food Chem., 51(15), 4247–4253. Biswas, T. (2021). Elicitor induced increased rosmarinic acid content of in vitro root cultures of Ocimum basilicum L. (Sweet Basil). Plant Sci. Today, 7(2), 157-163. Butler, N. M., Jansky, S. H., Jiang, J. (2020). First-generation genome editing in potato using hairy root transformation. Plant Biotechnol. J., 18(11), 2201-2209. Chandra, S. (2012). Natural plant genetic engineer Agrobacterium rhizogenes: role of T-DNA in plant secondary metabolism. Biotechnol. Lett., 34, 407-415. Cheng, J. C. (2003). 利用Rosmarinus officinalis懸浮細胞生產迷迭香酸之研究. 臺灣大學農業化學研究所學位論文, 1-79. Chung, C. H. (2010). 以圓葉菸草毛狀根表現Pinoresinol lariciresinol reductase與Secoisolariciresinol dehydrogenase. 臺灣大學分子與細胞生物學研究所學位論文, 1-57. Döring, A. S., Petersen, M. (2014). Production of caffeic, chlorogenic and rosmarinic acids in plants and suspension cultures of Glechoma hederacea. Phytochem. Lett., 10, cxi-cxvii. Ekstrom, K., Hoffman, K., Linne, T., Eriksson, B., Glimelius, B. (1998). Single-dose etoposide in advanced pancreatic and biliary cancer, a phase II study. Oncol. Rep., 5(4), 931-934. Faisal, M., Siddique, I., Anis, M. (2006). An efficient plant regeneration system for Mucuna pruriens L. (DC.) using cotyledonary node explants. In Vitro Cell. Dev. Biol. -Plant, 42, 59–64. Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., Mello, C. C. (1998). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391, 806-811. Giri, A., Narasu, M. (2000). Transgenic hairy roots: recent trends and applications. Biotechnol. Adv., 18(1), 1-22. Grayer, R. J., Bryan, S. E., Veitch, N. C., Goldstone, F. J., Paton, A., Wollenweber, E. (1996). External flavones in sweet basil, Ocimum basilicum, and related taxa. Phytochemistry, 43(5), 1041-1047. Gregory, R. I., Chendrimada, T. P., Cooch, N., Shiekhattar, R. (2005). Human RISC Couples MicroRNA Biogenesis and Posttranscriptional Gene Silencing. Cell, 123(4), 631-640. Habtemariam, S. (2018). Molecular Pharmacology of Rosmarinic and Salvianolic Acids: Potential Seeds for Alzheimer's and Vascular Dementia Drugs. Int. J. Mol. Sci., 19(2), 458. Hamilton, A. J., Baulcombe, D. C. (1999). A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants. Science, 286(5441), 950-952. Holm, B., Sehested, M., Jensen, P. B. (1998). Improved targeting of brain tumors using dexrazoxane rescue of topoisomerase II combined with supralethal doses of etoposide and teniposide. Clin. Cancer Res., 4(6), 1367-1373. Hücherig, S., Petersen, M. (2013). RNAi suppression and overexpression studies of hydroxyphenylpyruvate reductase (HPPR) and rosmarinic acid synthase (RAS) genes related to rosmarinic acid biosynthesis in hairy root cultures of Coleus blumei. Plant Cell Tiss. Organ Cult., 113, 375–385. Jayasinghe, C., Gotoh, N., Aoki, T., Wada, S. (2003). Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.). J. Agric. Food Chem., 51(15), 4442-4449. Kalita, R., Modi, M. K., Sen, P. (2018). RNAi mediated silencing of 3-hydroxy-3-methylglutaryl-CoA reductases (HMGR) in Centella asiatica. Gene Rep., 11, 52-57. Kruse, L. H., Stegemann, T., Jensen-Kroll, J., Engelhardt, A., Wesseling, A.-M., Lippert, A., . . . Ober, D. (2019). Reduction of Pyrrolizidine Alkaloid Levels in Comfrey (Symphytum officinale) Hairy Roots by RNAi Silencing of Homospermidine Synthase. Planta Med., 85, 1177-1186. Kwon, D. Y., Kim, Y. B., Kim, J. K., Park, S. U. (2021). Production of rosmarinic acid and correlated gene expression in hairy root cultures of green and purple basil (Ocimum basilicum L.). Prep. Biochem. Biotech., 51(1), 35-43. Levsh, O., Pluskal, T., Carballo, V., Mitchell, A. J., Weng, J.-K. (2019). Independent evolution of rosmarinic acid biosynthesis in two sister families under the Lamiids clade of flowering plants. J. Biol. Chem., 294(42), 15193-15205. Li, H., Fu, Y., Sun, H., Zhang, Y., Lan, X. (2017). Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum. Sci. Rep., 7(1), 74. Li, M., Sun, P., Kang, T., Xing, H., Yang, D., Zhang, J., W.Paré, P. (2018). Mapping podophyllotoxin biosynthesis and growth-related transcripts with high elevation in Sinopodophyllum hexandrum. Ind. Crops Prod., 124, 510-518. Li, W., Bai, Z., Pei, T., Yang, D., Mao, R., Zhang, B., . . . Liang, Z. (2019). SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza. Front. Plant Sci., 10, 1367. Lin, Y. Y. (2004). 應用RNA干擾技術抑制香蕉ACC氧化酶基因表現之研究. 臺灣大學園藝學研究所學位論文, 1-115. Lu, Y. S. (2019). 以分裂泛素酵母菌雙雜交系統篩選與RolB具交互作用之蛋白質. 臺灣大學生化科技學系學位論文, 1-77. Mandal, S. M., Chakraborty, D., Dey, S. (2010). Phenolic acids act as signaling molecules in plant-microbe symbioses. Plant Signal. Behav., 5(4), 359–368. Metsämuuronen, S., Sirén, H. (2019). Bioactive phenolic compounds, metabolism and properties: a review on valuable chemical compounds in Scots pine and Norway spruce. Phytochem. Rev., 18, 623–664. Petersen, M., Simmonds, M. S. (2003). Rosmarinic acid. Phytochemistry, 62(2), 121-125. Phippen, W. B., Simon, J. E. (1998). Anthocyanins in Basil (Ocimum basilicum L.). J. Agric. Food Chem., 46(5), 1734–1738. Rastogi, S., Kumar, R., Chanotiya, C. S., Shanker, K., Gupta, M. M., Nagegowda, D. A., Shasany, A. K. (2013). 4-Coumarate: CoA Ligase Partitions Metabolites for Eugenol Biosynthesis. Plant Cell Physiol., 54(8), 1238–1252. Renouard, S., Corbin, C., Drouet, S., Medvedec, B., Doussot, J., Colas, C., . . . Hano, C. (2018). Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans. Int. J. Mol. Sci., 19(4), 990. Scarpati, M. L., Oriente, G. (1958). Isolamento e costituzione dell’acido rosmarinico (dal rosmarinus off.). Ric. Sci., 28, 2329–2333. Sharafi, A., Sohi, H. H., Azadi, P., Sharafi, A. A. (2014). Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi. Physiol. Mol. Biol. Plants, 20(2), 257–262. Shi, H. P., Long, Y. Y., Sun, T. S., Tsang, P. K. (2011). Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin. Plant Cell Tiss. Organ Cult., 107, 251–260. Shi, M., Liao, P., Nile, S. H., Georgiev, M. I., Kai, G. (2021). Biotechnological Exploration of Transformed Root Culture for Value-Added Products. Trends Biotechnol., 39(2), 137-149. Singh, N. K., Sehgal, C. (1999). Micropropagation of ‘Holy Basil’ (Ocimum sanctum Linn.) from young inflorescences of mature plants. Plant Growth Regul., 29, 161–166. Smith, N. A., Singh, S. P., Wang, M.-B., Stoutjesdijk, P. A., Green, A. G., Waterhouse, P. M. (2000). Total silencing by intron-spliced hairpin RNAs. Nature, 407, 319-320. Song, J., Wang, Z. (2011). RNAi-mediated suppression of the phenylalanine ammonia-lyase gene in Salvia miltiorrhiza causes abnormal phenotypes and a reduction in rosmarinic acid biosynthesis. J. Plant Res., 124, 183–192. Spena, A., Schmülling, T., Koncz, C., Schell, J. S. (1987). Independent and synergistic activity of rol A, B and C loci in stimulating abnormal growth in plants. EMBO J., 6(13), 3891-3899. Tuan, P. A., Park, W. T., Xu, H., Park, N. I., Park, S. U. (2012). Accumulation of Tilianin and Rosmarinic Acid and Expression of Phenylpropanoid Biosynthetic Genes in Agastache rugosa. J. Agric. Food Chem., 60(23), 5945–5951. Vanholme, R., Meester, B. D., Ralph, J., Boerjan, W. (2019). Lignin biosynthesis and its integration into metabolism. Curr. Opin. Biotechnol., 56, 230-239. Wang, J. H. (2015). 菸草毛狀根的生長與其尼古丁高量累積之研究. 臺灣大學生化科技學系學位論文, 1-162. Wang, Q., Reddy, V. A., Panicker, D., Mao, H.-Z., Kumar, N., Rajan, C., . . . Sarojam, R. (2016). Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata). Plant Biotechnol. J., 14, 1619-1632. Wang, Y. M., Wang, J. B., Luo, D., Jia, J. F. (2001). Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi. Cell Res., 11, 279–284. Waterhouse, P. M., Graham, M. W., Wang, M. B. (1998). Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc. Natl. Acad. Sci. U. S. A., 95(23), 13959-13964. Wu, J., Wang, Y., Zhang, L.-X., Zhang, X.-Z., Kong, J., Lu, J., Han, Z.-H. (2012). High-efficiency regeneration of Agrobacterium rhizogenes-induced hairy root in apple rootstock Malus baccata (L.) Borkh. Plant Cell Tiss. Organ Cult., 111, 183–189. Xiao, Y., Zhang, L., Gao, S., Saechao, S., Di, P., Chen, J., Chen, W. (2011). The c4h, tat, hppr and hppd Genes Prompted Engineering of Rosmarinic Acid Biosynthetic Pathway in Salvia miltiorrhiza Hairy Root Cultures. PLoS One, 6(12), e29713. Zhang, B., Huo, Y., Zhang, J., Zhang, X., Zhu, C. (2019). Agrobacterium rhizogenes-mediated RNAi of Tripterygium wilfordii and application for functional study of terpenoid biosynthesis pathway genes. Ind. Crops Prod., 139, 111509. Zhang, S., Li, H., Liang, X., YanYan, Xia, P., Jia, Y., Liang, Z. (2015). Enhanced production of phenolic acids in Salvia miltiorrhiza hairy root cultures by combing the RNAi-mediated silencing of chalcone synthase gene with salicylic acid treatment. Biochem. Eng. J., 103, 185-192. Zhao, Y., Li, N., Li, B., Li, Z., Xie, G., Zhang, J. (2015). Reduced expression of starch branching enzyme IIa and IIb in maize endosperm by RNAi constructs greatly increases the amylose content in kernel with nearly normal morphology. Planta, 241(2), 449-461. Zhou, Z., Tan, H., Li, Q., Chen, J., Gao, S., Wang, Y., . . . Zhang, L. (2018). CRISPR/Cas9-mediated efficient targeted mutagenesis of RAS in Salvia miltiorrhiza. Phytochemistry, 148, 63-70. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79751 | - |
| dc.description.abstract | "植物次級代謝物是來自植物的小分子有機化合物，用於調和植物與環境的相互作用。植物次級代謝物同時在現代醫學中為重要的藥物，常作為癌症藥物等各種疾病藥物之先導化合物 (lead compounds)。其中，鬼臼素 (podophyllotoxin) 之衍生物能用於治療肺癌等多種癌症之化療藥物etoposide和teniposide。但鬼臼屬的植物生長緩慢且次級代謝物的產量不多，同時化學結構複雜，很難使用化學合成方法大量合成產物。因此本研究之目的為透過代謝工程之方法建構能生合成鬼臼素前驅物 (precursor) 之羅勒 (Ocimum basilicum)。目標基因為迷迭香酸合成酶 (Rosmarinic acid synthase, RAS)，一種催化4-Coumaroyl-coA和4-hydroxyphenyllactate合成迷迭香酸之酵素。透過RNA干擾 (RNA interference, RNAi) 的技術，下調羅勒中迷迭香酸 (rosmarinic acid) 的生合成，驅使代謝路徑上之前驅物走向生合成鬼臼素之路徑。將建構好的抑制RAS表現之質體pRASi轉形進根毛農桿菌 (Agrobacterium rhizogenes) ATCC15834中，並感染羅勒誘導RNAi毛狀根產生，同時用未帶有pRASi之農桿菌ATCC15834誘導的毛狀根做為控制組。挑選生長狀況好且側根多的毛狀根進行培養，獲得未進行RNAi之毛狀根HR5和HR12，以及具RNAi表現之毛狀根RASi21和RASi22。之後，以real-time PCR檢測RASi21和RASi22的RAS表現量，結果發現其RAS基因表現量較HR5和HR12顯著下降。再以UPLC測量目標產物迷迭香酸的累積量，顯示兩週大之RASi21和RASi22的迷迭香酸累積量最少僅有0.9983 mg/g DW，比起兩週大之HR5和HR12有到20.94 mg/g DW顯著降低。成功獲得經過RNAi之技術進行RAS基因沉默後的毛狀根RASi21和RASi22。此外，經過多種羅勒毛狀根再生 (regeneration) 之培養基嘗試，發現較適合添加之植物激素比例，未來有望將RASi21和RASi22再生為完整植株，以用於進一步的毛狀根誘導。再經由轉形鬼臼素生合成路徑上之基因進羅勒，以生產抗癌植物次級代謝物。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:09:50Z (GMT). No. of bitstreams: 1 U0001-1808202112181000.pdf: 8140722 bytes, checksum: 96f229ca59aa4f16d7c9d7bc474497c1 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "目錄 致謝 i 摘要 ii Abstract iii 縮寫表 v 目錄 vii 圖表目錄 ix 1. 前言 1 1.1. 毛狀根 (Hairy root) 1 1.2. 鬼臼素 (Podophyllotoxin) 2 1.3. 羅勒 4 1.4. RNA干擾 (RNA interference, RNAi) 7 1.5. 植物再生 (Plant regeneration) 8 1.6. 實驗目的 8 2. 材料與方法 10 2.1. 植物材料與毛狀根建立 10 2.2. DNA RNA 11 2.3. RAS基因沉默質體建構 14 2.4. RAS表現量之分析 21 2.5. 迷迭香酸之萃取與分析 21 2.6. 毛狀根之植株再生 (Regeneration) 22 3. 結果 29 3.1. 野生型羅勒毛狀根HR5與HR12 29 3.2. 建立RASi毛狀根RASi21與RASi22 29 3.3. RAS表現量之分析 31 3.4. 迷迭香酸累積量之分析 31 3.5. 羅勒毛狀根之再生 33 4. 討論 49 4.1. 羅勒無菌植株生長狀況與迷迭香酸含量的關係 49 4.2. RASi毛狀根的建立 49 4.3. 植物中RNAi用於了解機制和代謝物的生產 50 4.4. RASi21和RASi22中RAS表現量與代謝物上的差異 51 4.5. 未來應用性 51 5. 結論 53 參考資料 54" | |
| dc.language.iso | zh-TW | |
| dc.subject | 迷迭香酸合成酶 | zh_TW |
| dc.subject | 迷迭香酸 | zh_TW |
| dc.subject | 羅勒 | zh_TW |
| dc.subject | RNA干擾 | zh_TW |
| dc.subject | 毛狀根 | zh_TW |
| dc.subject | rosmarinic acid synthase (RAS) | en |
| dc.subject | RNAi | en |
| dc.subject | hairy root | en |
| dc.subject | rosmarinic acid | en |
| dc.subject | Ocimum basilicum | en |
| dc.title | 羅勒毛狀根中迷迭香酸合成酶之RNAi基因沉默之研究 | zh_TW |
| dc.title | RNAi-Mediated Silencing of Rosmarinic Acid Synthase in Hairy Roots of Ocimum basilicum | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊健志(Hsin-Tsai Liu),黃鵬林(Chih-Yang Tseng),劉啟德,賴爾珉 | |
| dc.subject.keyword | 迷迭香酸,迷迭香酸合成酶,羅勒,RNA干擾,毛狀根, | zh_TW |
| dc.subject.keyword | rosmarinic acid,rosmarinic acid synthase (RAS),Ocimum basilicum,RNAi,hairy root, | en |
| dc.relation.page | 59 | |
| dc.identifier.doi | 10.6342/NTU202102463 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-08-20 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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