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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王俊能(Chun-Neng Wang) | |
dc.contributor.author | Ya-Chi Nien | en |
dc.contributor.author | 粘雅淇 | zh_TW |
dc.date.accessioned | 2021-07-11T15:33:52Z | - |
dc.date.available | 2021-08-20 | |
dc.date.copyright | 2018-08-21 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-15 | |
dc.identifier.citation | Aida, M., T. Ishida, H. Fukaki, H. Fujisawa and M. Tasaka (1997). 'Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant.' Plant Cell 9(6): 841-857.
Blazquez, M. A., C. Ferrandiz, F. Madueno and F. Parcy (2006). 'How floral meristems are built.' Plant Mol Biol 60(6): 855-870. Busch, A. and S. Zachgo (2009). 'Flower symmetry evolution: towards understanding the abominable mystery of angiosperm radiation.' Bioessays 31(11): 1181-1190. D'Ario, M., S. Griffiths-Jones and M. Kim (2017). 'Small RNAs: Big Impact on Plant Development.' Trends Plant Sci 22(12): 1056-1068. Ellis, C. M., P. Nagpal, J. C. Young, G. Hagen, T. J. Guilfoyle and J. W. Reed (2005). 'AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana.' Development 132(20): 4563-4574. Hackenberg, M., A. Rueda, P. Gustafson, P. Langridge and B. J. Shi (2016). 'Generation of different sizes and classes of small RNAs in barley is locus, chromosome and/or cultivar-dependent.' BMC Genomics 17(1): 735. Hashimoto, Y., Y. Akiyama and Y. Yuasa (2013). 'Multiple-to-multiple relationships between microRNAs and target genes in gastric cancer.' PLoS One 8(5): e62589. Hileman, L. C. (2014). 'Trends in flower symmetry evolution revealed through phylogenetic and developmental genetic advances.' Philos Trans R Soc Lond B Biol Sci 369(1648). Hu, Z., Q. Jiang, Z. Ni, R. Chen, S. Xu and H. Zhang (2013). 'Analyses of a Glycine max degradome library identify microRNA targets and microRNAs that trigger secondary siRNA biogenesis.' J Integr Plant Biol 55(2): 160-176. Jeong, D. H., S. A. Schmidt, L. A. Rymarquis, S. Park, M. Ganssmann, M. A. German, M. Accerbi, J. Zhai, N. Fahlgren, S. E. Fox, D. F. Garvin, T. C. Mockler, J. C. Carrington, B. C. Meyers and P. J. Green (2013). 'Parallel analysis of RNA ends enhances global investigation of microRNAs and target RNAs of Brachypodium distachyon.' Genome Biol 14(12): R145. Jones-Rhoades, M. W. and D. P. Bartel (2004). 'Computational identification of plant microRNAs and their targets, including a stress-induced miRNA.' Mol Cell 14(6): 787-799. Juarez, M. T., J. S. Kui, J. Thomas, B. A. Heller and M. C. Timmermans (2004). 'microRNA-mediated repression of rolled leaf1 specifies maize leaf polarity.' Nature 428(6978): 84-88. Kang, M., Q. Zhao, D. Zhu and J. Yu (2012). 'Characterization of microRNAs expression during maize seed development.' BMC Genomics 13: 360. Kidner, C. A. and R. A. Martienssen (2004). 'Spatially restricted microRNA directs leaf polarity through ARGONAUTE1.' Nature 428(6978): 81-84. Li, R., D. Chen, T. Wang, Y. Wan, R. Li, R. Fang, Y. Wang, F. Hu, H. Zhou, L. Li and W. Zhao (2017). 'High throughput deep degradome sequencing reveals microRNAs and their targets in response to drought stress in mulberry (Morus alba).' PLoS One 12(2): e0172883. Li, T., H. Li, Y. X. Zhang and J. Y. Liu (2011). 'Identification and analysis of seven H(2)O(2)-responsive miRNAs and 32 new miRNAs in the seedlings of rice (Oryza sativa L. ssp. indica).' Nucleic Acids Res 39(7): 2821-2833. Li, Y. F., Y. Zheng, C. Addo-Quaye, L. Zhang, A. Saini, G. Jagadeeswaran, M. J. Axtell, W. Zhang and R. Sunkar (2010). 'Transcriptome-wide identification of microRNA targets in rice.' Plant J 62(5): 742-759. Lin, P. C., C. W. Lu, B. N. Shen, G. Z. Lee, J. L. Bowman, M. A. Arteaga-Vazquez, L. Y. Liu, S. F. Hong, C. F. Lo, G. M. Su, T. Kohchi, K. Ishizaki, S. Zachgo, F. Althoff, M. Takenaka, K. T. Yamato and S. S. Lin (2016). 'Identification of miRNAs and Their Targets in the Liverwort Marchantia polymorpha by Integrating RNA-Seq and Degradome Analyses.' Plant Cell Physiol 57(2): 339-358. Lin, Y., L. Lin, R. Lai, W. Liu, Y. Chen, Z. Zhang, X. XuHan and Z. Lai (2015). 'MicroRNA390-Directed TAS3 Cleavage Leads to the Production of tasiRNA-ARF3/4 During Somatic Embryogenesis in Dimocarpus longan Lour.' Front Plant Sci 6: 1119. Liu, N., L. Tu, L. Wang, H. Hu, J. Xu and X. Zhang (2017). 'MicroRNA 157-targeted SPL genes regulate floral organ size and ovule production in cotton.' BMC Plant Biol 17(1): 7. Liu, Y. X., M. Wang and X. J. Wang (2014). 'Endogenous small RNA clusters in plants.' Genomics Proteomics Bioinformatics 12(2): 64-71. Marin, E., V. Jouannet, A. Herz, A. S. Lokerse, D. Weijers, H. Vaucheret, L. Nussaume, M. D. Crespi and A. Maizel (2010). 'miR390, Arabidopsis TAS3 tasiRNAs, and their AUXIN RESPONSE FACTOR targets define an autoregulatory network quantitatively regulating lateral root growth.' Plant Cell 22(4): 1104-1117. Mi, S., T. Cai, Y. Hu, Y. Chen, E. Hodges, F. Ni, L. Wu, S. Li, H. Zhou, C. Long, S. Chen, G. J. Hannon and Y. Qi (2008). 'Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide.' Cell 133(1): 116-127. Moss, E. G. (2007). 'Heterochronic genes and the nature of developmental time.' Curr Biol 17(11): R425-434. Pais, H., S. Moxon, T. Dalmay and V. Moulton (2011). 'Small RNA discovery and characterisation in eukaryotes using high-throughput approaches.' Adv Exp Med Biol 722: 239-254. Palatnik, J. F., E. Allen, X. Wu, C. Schommer, R. Schwab, J. C. Carrington and D. Weigel (2003). 'Control of leaf morphogenesis by microRNAs.' Nature 425(6955): 257-263. Pasquinelli, A. E. and G. Ruvkun (2002). 'Control of developmental timing by micrornas and their targets.' Annu Rev Cell Dev Biol 18: 495-513. Rajagopalan, R., H. Vaucheret, J. Trejo and D. P. Bartel (2006). 'A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana.' Genes Dev 20(24): 3407-3425. Rhoades, M. W., B. J. Reinhart, L. P. Lim, C. B. Burge, B. Bartel and D. P. Bartel (2002). 'Prediction of plant microRNA targets.' Cell 110(4): 513-520. Rougvie, A. E. (2005). 'Intrinsic and extrinsic regulators of developmental timing: from miRNAs to nutritional cues.' Development 132(17): 3787-3798. Rubio-Somoza, I. and D. Weigel (2011). 'MicroRNA networks and developmental plasticity in plants.' Trends Plant Sci 16(5): 258-264. Szittya, G., S. Moxon, D. M. Santos, R. Jing, M. P. Fevereiro, V. Moulton and T. Dalmay (2008). 'High-throughput sequencing of Medicago truncatula short RNAs identifies eight new miRNA families.' BMC Genomics 9: 593. Todesco, M., I. Rubio-Somoza, J. Paz-Ares and D. Weigel (2010). 'A collection of target mimics for comprehensive analysis of microRNA function in Arabidopsis thaliana.' PLoS Genet 6(7): e1001031. Wang, C. N., H. C. Hsu, C. C. Wang, T. K. Lee and Y. F. Kuo (2015). 'Quantifying floral shape variation in 3D using microcomputed tomography: a case study of a hybrid line between actinomorphic and zygomorphic flowers.' Front Plant Sci 6: 724. Wang, H. and H. Wang (2015). 'The miR156/SPL Module, a Regulatory Hub and Versatile Toolbox, Gears up Crops for Enhanced Agronomic Traits.' Mol Plant 8(5): 677-688. Wang, Y., Y. Ding and J. Y. Liu (2016). 'Identification and Profiling of microRNAs Expressed in Elongating Cotton Fibers Using Small RNA Deep Sequencing.' Front Plant Sci 7: 1722. Wang, Y., Y. Ding, D. Yu, W. Xue and J. Liu (2015). 'High-throughput sequencing-based genome-wide identification of microRNAs expressed in developing cotton seeds.' Sci China Life Sci 58(8): 778-786. Xia, R., J. Xu and B. C. Meyers (2017). 'The Emergence, Evolution, and Diversification of the miR390-TAS3-ARF Pathway in Land Plants.' Plant Cell 29(6): 1232-1247. Xia, R., S. Ye, Z. Liu, B. C. Meyers and Z. Liu (2015). 'Novel and Recently Evolved MicroRNA Clusters Regulate Expansive F-BOX Gene Networks through Phased Small Interfering RNAs in Wild Diploid Strawberry.' Plant Physiol 169(1): 594-610. Xia, R., H. Zhu, Y. Q. An, E. P. Beers and Z. Liu (2012). 'Apple miRNAs and tasiRNAs with novel regulatory networks.' Genome Biol 13(6): R47. Yang, C. Y., Y. H. Huang, C. P. Lin, Y. Y. Lin, H. C. Hsu, C. N. Wang, L. Y. Liu, B. N. Shen and S. S. Lin (2015). 'MicroRNA396-Targeted SHORT VEGETATIVE PHASE Is Required to Repress Flowering and Is Related to the Development of Abnormal Flower Symptoms by the Phyllody Symptoms1 Effector.' Plant Physiol 168(4): 1702-1716. Zhong, R. and Z. H. Ye (2007). 'Regulation of HD-ZIP III Genes by MicroRNA 165.' Plant Signal Behav 2(5): 351-353 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78979 | - |
dc.description.abstract | 花朵性狀的多樣發育變化形成開花植物在地球上繁盛出近三十萬種的多樣性,其中,演化出花朵兩側對稱性的物種,具有相異的背腹側花瓣形態,不但使花形變異更特化,花朵傳粉性狀特化也促進植物與昆蟲共演化。然而,對於建立背腹側花瓣極性的分子機制,目前學界尚未完全知曉。MicroRNAs (miRNAs)為一種內生的單股、非編碼的小片段RNA,長度約20-24個鹼基對。許多研究發現,miRNAs參與生物的基因表現調控,但目前尚未有研究在背腹側花瓣中探討miRNAs的調控。本實驗以野生型的大岩桐原種 (Sinningia speciosa) 兩側對稱花為材料,結合次世代定序與生物資訊分析,發現157個miRNAs表現於背腹側花瓣中。其中,不僅包含尚未在苦苣苔科發表過的conserved miRNAs,同時也有95個未曾在其他物種發表的novel miRNAs。有趣的是,我們發現miR157與miR390在背腹側花瓣有差異性表現,兩者在腹側花瓣皆偵測到較高的表現量。透過降解體定序,我們分別確認miR157與miR390的目標基因為SPLs與TAS3。SPLs曾被報導透過調控auxin-responsive genes來決定花器的大小;TAS3則是藉由產生ta-siRNAs,降解ARF3, ARF4,同樣調控auxin-responsive genes,以建立葉片上下表面的極性。轉錄體定序資料顯示,大岩桐的SPLs與ARF3同樣也具有背腹側表現差異。接著,我們進行stem-loop qPCR,再次驗證了定序資料的結果。綜合以上,本研究顯示miR157/SPLs與miR390/ARF3的調控路徑參與大岩桐花瓣發育。同時,此二路徑藉由調控下游的auxin-responsive genes,可能促使背腹側花瓣相異的形態發生。進一步,我們的發現也顯示,原先參與葉片上下表面極性的分子調控,可能擴展適應到建立花原基背腹側的極性。本實驗由轉譯後層次探討植物背腹側花瓣的差異分化,及其對於後續兩側對稱性的貢獻,有助於我們對於植物體花朵對稱性有更完整且全面的了解。 | zh_TW |
dc.description.abstract | Establishment of dorsi-ventral polarity is essential for zygomorphic flower development, which contributes to the evolution of specialized pollination syndrome thus enhances species richness. But the mechanisms underlying the polarity establishment remain unclear. MicroRNAs (miRNAs) are embedded in the regulatory networks of plant development, but direct evidence for miRNAs regulating dorsi-ventral petal morphogenesis has been lacking.
Here, we investigated the dorsi-ventrally expressed miRNAs in the zygomorphic flower of Sinningia speciosa, an economically important ornamental plant. Using a computational pipeline, we identified 157 miRNAs in developing petals, including both conserved miRNAs that are reported for the first time in S. speciosa, as well as 95 novel miRNAs that are not found in other plants. Interestingly, we discovered that miR157 and miR390 appeared more abundant in ventral petal than dorsal part. A combination of PARE analysis allowed us to validate their targets, respectively. SPLs, which are reported to be involved in regulating the floral organ size by controlling auxin-responsive genes, and TAS3, which have been invoked in the control of the specification of abaxial/adaxial polarity of leafs by generating ta-siRNAs, which in turn cause degradation of the AUXIN RESPONSE FACTOR 3 (ARF3) and ARF4 mRNAs. Intriguingly, our transcriptome data indicated that both SPLs and ARF3 show dorsi-ventral expression pattern. A similar result was also observed when we experimentally validated the expression patterns by qPCR. Our findings indicated that the regulation of miR157/SPLs may regulate the auxin-responsive genes, thus contribute to the different morphogenesis of dorsal and ventral petals. As for miR390/ARF3, the establishment of the ta-siRNA polarity is similar to the findings in leaves, which is a crucial determinant of abaial/adaxial polarity development. As a result, our finding raises an intriguing possibility of co-option in the development of leaf abaxial-adaxial polarity into floral dorsi-ventral zygomorphy. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T15:33:52Z (GMT). No. of bitstreams: 1 ntu-107-R05b44011-1.pdf: 4179878 bytes, checksum: 051250eefc86415ec59b6e5779c2c57c (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 中文摘要 I
Abstract II 目錄 IV 圖目錄 V 表目錄 VI Introduction 1 Results 6 Discussion 32 Conclusion 43 Materials and Methods 44 Reference 49 Supplementary data 53 | |
dc.language.iso | en | |
dc.title | 微小核酸對大岩桐花朵對稱性的調控 | zh_TW |
dc.title | Global analysis of small RNAs for controlling
floral symmetry in Sinningia speciosa | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 陳荷明(Ho-Ming Chen) | |
dc.contributor.oralexamcommittee | 陳仁治(Jen-Chih Chen),林詩舜(Shih-Shun Lin) | |
dc.subject.keyword | 大岩桐,兩側對稱性,微小核糖核酸,次世代定序, | zh_TW |
dc.subject.keyword | Sinningia,zygomorphy,next-generation sequencing,microRNAs, | en |
dc.relation.page | 59 | |
dc.identifier.doi | 10.6342/NTU201803727 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生態學與演化生物學研究所 | zh_TW |
顯示於系所單位: | 生態學與演化生物學研究所 |
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