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標題: | 探討化學修飾之金雀異黃酮與游泳訓練對於卵巢摘除大鼠骨質疏鬆的影響 Effect on Chemically Modified Genistein and Swimming Training on Ovariectomy-induced Osteoporosis |
作者: | 舒暢 Chang Shu |
指導教授: | 謝淑貞 |
關鍵字: | 絕經後骨質疏鬆症,金雀異黃酮磷酸酯,游泳訓練,協同作用, postmenopausal osteoporosis,G7P,swimming training,synergistic effect, |
出版年 : | 2019 |
學位: | 碩士 |
摘要: | 骨質疏鬆症 (Osteoporosis, OP) 是一種因骨骼強度減弱致使個人增加骨折危險性的疾病。絕經後骨質疏鬆症,又稱為第一型骨質疏鬆症,常見於停經後的婦女,其主要的致病原因是雌激素缺乏所導致的骨吸收增加。金雀異黃酮 (Genistein) 是大豆異黃酮中的一種主要活性成分,廣泛存在於豆類、穀類、水果、蔬菜等300多種植物中。其化學結構相似於卵巢分泌的17β-雌二醇,因此具備類雌激素活性的生理功能。然而,金雀異黃酮的生物可利用率較低,已有研究利用納豆菌發酵進行生物轉換,在其結構中加入磷酸根,形成金雀異黃酮磷酸酯 (Genistein 7- O-phosphate,G7P) ,可大幅提升化合物之水溶性而增加生物可利用率,從而增進其抗骨質疏鬆的潛力。規律且持續的運動也能避免骨質流失,甚至可以增加骨骼中礦物質的含量,提高骨骼強度。游泳是一種全身性的運動,可以讓人體全身的骨骼與肌肉得到協調性的鍛鍊,並且由於水中浮力的存在使得關節的承重降低,因而有保護關節的作用。
本實驗以卵巢摘除母鼠模式模擬絕經後骨質疏鬆症,在誘導骨鬆13週後各處理組分別管餵G7P、游泳訓練和兩者加成,與控制組做比較,探討兩者對於骨質疏鬆的治療是否具有協同作用。實驗結束後用ELISA法測血清中骨轉換指標PINP、TRAcP 5b的濃度,用微電腦斷層掃描儀測定股骨特定部位的BMD以及TB的相關指標,用股骨的組織病理切片來評估骨組織結構變化以及使用骨應力測驗機來測定脛骨的生物力學特性。實驗結果顯示,與Sham組相比,OVX組各項骨指標降低,造成骨質疏鬆的發生。G7P的攝取對於骨質的改善效果不明顯,部分指標來看甚至會加重骨質疏鬆的情形,而游泳訓練對於骨鬆有改善的趨勢,但是G7P加上游泳訓練組別相較於兩個單一處理組有更佳的骨質改善效果,特別是在股骨微電腦斷層掃描圖、股骨組織病理切片和脛骨生物力學上都有統計意義上的差異。因此,後續的研究可以從機制上探討為何游泳訓練和G7P對卵巢摘除大鼠骨質疏鬆有協同的治療效果。 Osteoporosis is a disease that increases the risk of fractures due to reduced bone strength. Postmenopausal osteoporosis, also known as type I osteoporosis, is commonly found in menopausal women whose major pathogenic factor is estrogen deficiency, leading to an increase in bone resorption. Genistein is one of the main active compounds among soybean isoflavones, which is widely found in more than 300 kinds of plants such as legumes, cereals, fruits and vegetables. Its chemical structure is similar to the 17β-estradiol secreted by ovaries, and thus contributes to its estrogen-like physiological function. The low bioavailability of genistein could be improved through biotransformation that is carried out using Bacillus subtilis fermentation. A phosphate group is added to its original structure to form genistein 7-O-phosphate (G7P); the higher water solubility and increased bioavailability makes it a potential anti-osteoporotic material. Regular and sustained exercise can also reduce bone loss, and even can improve bone strength by increasing the bone minerals. Swimming is one of the exercises that can move most parts of bones and muscles, thus improving overall coordination. Study also showed that buoyancy in water could help in protecting the joints through the reduction of joint load. In this study, ovariectomized (OVX) rats were used to model postmenopausal osteoporosis. After 13 weeks of induction, oral administration of G7P, swimming training and their combination were applied to rats daily to investigate whether the combination of oral administration with G7P and swimming training has a synergistic effect on preventing postmenopausal osteoporosis. At the end of the experiment, the levels of bone turnover markers PINP and TRAcP 5b were measured by ELISA. BMD and TB related indexes were determined by microcomputed tomography. The changes of bone structure were evaluated by histological sections of femur and bone stress tester was used to determine the biomechanical properties of the tibia. Experimental results showed that bone indicators of OVX group decreased and resulted in the occurrence of osteoporosis compared with sham group. Swimming training alone showed a positive trend for attenuating bone loss compared with OVX control, whereas treatment of G7P alone did not showed any significant improvement on postmenopausal osteoporosis. However, the combination of G7P and swimming training significantly improved bone indices compared to two other groups, showing significant differences in parameters measured by femoral microcomputed tomography, femoral histomorphometry and tibial biomechanics. Hence, this study can be used as a starting point to further explore the mechanism on how the combination of G7P together with swimming training improve osteoporosis in the ovariectomized rat model. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78866 |
DOI: | 10.6342/NTU201900023 |
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顯示於系所單位: | 食品科技研究所 |
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