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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭光成(Kuan-Chen Cheng) | |
| dc.contributor.author | Ching-Hsiang Lin | en |
| dc.contributor.author | 林清翔 | zh_TW |
| dc.date.accessioned | 2021-07-11T14:48:13Z | - |
| dc.date.available | 2025-08-19 | |
| dc.date.copyright | 2020-08-28 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-13 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78259 | - |
| dc.description.abstract | 羅漢果 (Siraitia grosvenorii) 為中國廣西省桂林市特產之一，可作為中藥材使用，具有潤肺、舒緩咳嗽及預防便祕等功效。近年來，羅漢果皂苷相關的研究日漸增加，其中指出siamenoside Ⅰ具有比5%蔗糖水溶液563倍的甜度，且相較於其他羅漢果皂苷風味較佳，更適合作為甜味劑使用。mogroside Ⅳ可減緩肺纖維化症狀與抑制癌細胞增殖，在生醫藥產業中極具發展潛力。於先前的研究中常以化學水解的方式進行皂苷的轉換，但使用化學水解有環境危害及產量低的問題，故本研究目的為建立一固定化酵素的連續式生物反應器以生產siamenoside Ⅰ和mogroside Ⅳ，降低環境危害及有效提高產量並增加酵素再利用性。結果顯示固定化β-葡萄糖苷酶在戊二醛濃度1.5%、載體活化時間1小時及結合酵素時間12小時有最佳的相對活性，並且在60oC、pH 5的環境下有最佳反應活性，後續填充已固定β-葡萄糖苷酶之玻璃微粒（glass microspheres）至反應器中並維持最佳活性環境，利用控制流速的方式通入羅漢果萃取物水溶液，以0.3 mL/min的流速下可得最高濃度之siamenoside Ⅰ；0.2 mL/min的流速下可得最高濃度之mogroside Ⅳ。藉由開發此種固定化酵素系統以製備大量且純度高之siamenoside Ⅰ與mogroside Ⅳ。 | zh_TW |
| dc.description.abstract | Siraitia grosvenorii (Swingle) is abundant in Guangxi province, China. Several studies have reported its benefits, such as moisturizing lungs, soothing coughs, reducing blood pressure and preventing constipation. Recently, research related to mogroside is increasing. Siamenoside I has been reported to be 563 times sweeter than 5% sucrose solution without bitterness compared to others; Mogroside IV has also been found several positive effects for attenuating liver fibrosis and suppressing the proliferation of cancer cells. Mogroside IV shows high potential to be applied in pharmaceutical industry. Traditionally, the most common conversion method is acid hydrolysis which has some drawback, such as low efficiency and polluted environment. On the contrary, immobilized enzyme shows high specificity, reusability and stability. Therefore, our objective is to develop immobilized enzyme bioreactor system to produce the siamenoside Ⅰ and mogroside Ⅳ as well as improving its productivity. The results show that the optimal conditions of immobilization with glutaraldehyde concentration 1.5%, activation time 1 hour and coupling time 12 hours. Moreover, immobilized enzyme has the highest relative activity at 60℃ and pH 5. Subsequently, the glass microsphere with immobilized β-glucosidase was filled into the reactor and the optimal environment was maintained. We found that the most content of siamenoside I was transformed in feed rate 0.3 mL/min and mogroside IV was in 0.2 mL/min through introducing the solution of Luo Han Kuo extract. | en |
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| dc.description.tableofcontents | 摘要 iii Abstract iv 目錄 v 壹、前言 1 貳、文獻回顧 2 2.1 皂素 2 2.1.1 來源 2 2.1.2 結構與分類 2 2.1.2 生理活性 3 2.2 羅漢果 (Siraitia grosvenorii Swingle) 4 2.2.1 羅漢果簡介 4 2.2.2 羅漢果皂苷 5 2.2.3 Mogroside IV、siamenoside I之生理活性 7 2.2.4 Mogroside IV、Siamenoside I形成機制 7 2.3 β-葡萄糖苷酶 9 2.3.1 簡介 9 2.3.2 活性檢測方法 9 2.3.3 β-D-葡萄糖苷酶應用 10 2.4 酵素固定化 11 2.4.1 簡介及特點 11 2.4.2 酵素固定化之載體 11 2.4.3 酵素固定化之方法 12 2.4.4 酵素固定化於食品工業之應用 16 2.4.5 酵素固定化近年相關研究 18 2.5 生物反應器 21 2.5.1 生物反應器介紹及種類 21 2.5.2 酵素固定化之生物反應器應用 21 參、研究目標與假設 23 3.1 研究目的 23 3.2 研究架構 23 3.3 圖像式摘要 24 肆、材料與方法 25 4.1 試驗藥品 25 4.2 設備儀器 26 4.3 實驗方法 27 4.3.1 玻璃微粒前處理 27 4.3.2 戊二醛最佳固定條件 27 4.3.3 固定化酵素 27 4.3.4 表面元素及化學鍵結分析 28 4.3.5 掃描式電子顯微鏡 28 4.3.6 比較最佳反應溫度及pH值 28 4.3.7 評估重複利用性及貯存穩定性 29 4.3.8 酵素動力學參數 29 4.3.9 生物反應器架設 29 4.3.10 羅漢果萃取方法探討 30 4.3.11 利用生物反應器將mogroside V轉換成siamenoside I和mogroside IV 31 4.3.12 羅漢果萃取物水解後之樣品分析前處理 31 4.3.13 高效液相層析儀分析 31 4.3.14 統計分析 31 伍、結果與討論 32 5.1 固定化酵素 32 5.1.1 固定酵素後之載體表面觀察 32 5.1.2 固定酵素後之載體鍵結情形 32 5.1.3 戊二醛固定最佳條件 36 5.1.4 固定化酵素之載體吸附量 40 5.2 載體評估 41 5.2.1 對硝基苯吸光值對濃度之檢量線 41 5.2.2 探討最佳反應環境條件 42 5.2.3 比較酵素動力學參數 45 5.2.4 重複利用性 50 5.2.5 貯存穩定性 50 5.3 利用生物反應器生產Siamenoside I、Mogroside IV 53 5.3.1 羅漢果皂苷萃取方式探討 53 5.3.2 Mogroside V、Siamenoside I、Mogroside IV和Mogroside IIIE之定量檢量線 55 5.3.3 以固定化酵素進行羅漢果皂苷之轉換 58 5.3.4生物反應器之架設 62 5.3.5利用生物反應器探討不同流速下羅漢果皂苷轉換結果 62 陸、結論與未來展望 65 柒、參考文獻 66 附錄 76 附錄一、人工神經網路 76 1.1 人工神經網路介紹及發展 76 1.2 人工神經網路之應用 77 1.3 以不同參數調整生物反應器 78 附錄二、個人簡歷表 80 附錄三、論文抄襲檢測結果 81 | |
| dc.language.iso | zh-TW | |
| dc.subject | 羅漢果苷Ⅳ | zh_TW |
| dc.subject | 賽門苷Ⅰ | zh_TW |
| dc.subject | 固定化酵素 | zh_TW |
| dc.subject | β-葡萄糖苷酶 | zh_TW |
| dc.subject | 羅漢果皂苷 | zh_TW |
| dc.subject | 羅漢果 | zh_TW |
| dc.subject | mogroside Ⅳ | en |
| dc.subject | siamenoside Ⅰ | en |
| dc.subject | immobilized enzyme | en |
| dc.subject | β-glucosidase | en |
| dc.subject | mogrosides | en |
| dc.subject | luo han kuo | en |
| dc.title | 建立固定化β-葡萄糖苷酶系統生產賽門苷Ⅰ和羅漢果苷Ⅳ | zh_TW |
| dc.title | Production of Siamenoside Ⅰ and Mogroside Ⅳ from Siraitia grosvenorii using immobilized β-glucosidase system | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳錦樹(Chin-Shuh Chen),謝寶全(Pao-Chuan Hsieh),蔣欣翰,蘇正元 | |
| dc.subject.keyword | 羅漢果,羅漢果皂苷,β-葡萄糖苷酶,固定化酵素,賽門苷Ⅰ,羅漢果苷Ⅳ, | zh_TW |
| dc.subject.keyword | luo han kuo,mogrosides,β-glucosidase,immobilized enzyme,siamenoside Ⅰ,mogroside Ⅳ, | en |
| dc.relation.page | 81 | |
| dc.identifier.doi | 10.6342/NTU202003068 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2020-08-13 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| dc.date.embargo-lift | 2025-08-19 | - |
| 顯示於系所單位： | 食品科技研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-1208202011565600.pdf 未授權公開取用 | 3.14 MB | Adobe PDF |
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