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標題: | 矽水膠人工角膜襯裙之開發與測試 Development and Characterization of Keratoprosthesis Skirt Using Silicone-Hydrogel Material |
作者: | Hsin-Yi Duan 段欣怡 |
指導教授: | 王勝仕 |
關鍵字: | 水膠,矽成分,角膜襯裙,生醫材料, Hydrogel,Silicone-containing,cornea skirt,biomaterial, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | 角膜為一無血管、折射光線之透明組織,並有提供眼睛外層屏障等多種功能。目前由文獻統計顯示因疾病或創傷所造成之角膜致盲已影響了數千萬人口的生活,大多數人皆以角膜移植的手術方式作為視力回復的手段,然而過去有過失敗的移植經驗、角膜血管化等高風險患者若進行角膜移植手術,因排斥導致失敗的機率相當高而不適用,另一方面,由於現今雷射手術的發達以及疾病感染的增加,致使健康的捐贈角膜也日漸缺乏,因此人工角膜的發展成為未來的必然走向,也逐漸成為患者的新希望。
人工角膜一般可以分成兩個部分:中央光學區與幫助眼表代謝、固定光學區之角膜襯裙。於本研究中,吾人使用聚乙二醇雙丙烯酸酯(polyethylene glycol diacrylate, PEGDA)、泊洛沙姆407雙丙烯酸酯(Poloxamer 407, P407DA)以及單-(甲基丙烯酰氧丙基)聚二甲基矽氧烷(Mono-methacryloxypropyl terminated polydimethylsiloxane, mPDMS)之巨分子單體,以不同比例的配方混合後光交聯製備具多孔性的水膠材料。 研究結果可以發現以PEGDA為主要成分加入其他兩者材料時,初步以澎潤測試,當加入mPDMS時會使原先較親水的結構變得稍微疏水一些而使澎潤比下降,但隨著P407DA的使用量增加,會使澎潤性質逐漸回復近只有PEGDA單體製成水膠的程度。由掃描式電子顯微鏡 (SEM)直接觀察水膠樣品皆具開放性的多孔結構,並於加入mPDMS後孔洞尺寸顯著變小,而P407DA加入時則使孔洞變大,此結果在孔隙分析的部分相互呼應。在動態機械性質方面,以流變儀測量剪力儲存模數,只以PEGDA與mPDMS作成的水膠機械性質強度明顯增加,而P407DA加入,孔洞的尺寸逐漸上升,其機械性質的強度隨之變低。在加速氧化測試的結果中,只要mPDMS的水膠其被氧化使結構破碎的時間皆較長,但當P407DA加入至一定程度時,可能因孔洞變大而使結構較脆弱而使水膠破碎的時間變短。另一方面,於大鼠的皮下植入結果顯示,當水膠的孔徑越大時周圍之細胞聚集越多,而材料降解的情形仍然發生。總體來說,以含矽材料加入水膠之中能夠增進機械性質並延長降解時間,雖然以生物實驗結果上不甚理想,但高孔隙率以及大孔洞的特性,仍舊使本研究中的水膠樣品具有作為人工角膜襯裙之潛力存在。 The human cornea is a non-vascularized and optically transparent tissue with appropriate curvature surface that refracts light rays and serves as the outermost protective barrier of eye. Corneal opaci-fication from disease or trauma is estimated to affect more than 10 million people worldwide, and corneal transplant has become the most common surgical treatment to recover patients’ vision. However, corneal transplant is not applicable for patients with high-risks (e.g., ones who suffered from previous graft rejection, vascularization of recipient cornea, etc.), and thus, artificial cornea becomes a viable option. Artificial cornea is divided into two parts: the optical center and a ring of keratoprosthesis skirt. The keratoprosthesis skirt fixes the artificial cornea to the host sclera and serves to transfer nutrients and maintains ocular surface metabolism. In this study, three hydrogel components were tested as potential materials to be used for the construction of keratoprosthesis skirt: 1) polyethylene glycol diacrylate (PEGDA) and 2) poloxamer 407 diacrylate (P407DA) make up the high porosity framework, while 3) silicone-containing mono-methacryloxypropyl terminated polydimethylsiloxane’s (mPDMS) properties improves oxygen permeability and mechanical strength. Based on the swelling test, hydrogels containing mPDMS material were more hydrophobic than the ones containing PEGDA. Direct observation under the scanning electron microscope (SEM) and mercury porosimeter revealed that all hydrogels possess porous structure with open pores. The application of different amounts of P407DA can regulate pore size and porosity. According to the dynamic mechanical analysis, only hydrogels containing PEGDA and mPDMS have larger shear storage modulus, and that increasing the amount of P407DA de-creases the shear storage modulus. The accelerated oxidation test showed that hydrogels containing mPDMS have greater stability (longer degradation time) than the ones containing PEGDA. In conclusion, silicone-based material can promote mechanical strength and prolong degrada-tion time. We believe the high porosity and large open pores in these hydrogels have the potential to serve as the raw material for keratoprosthesis skirt of artificial cornea. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78169 |
DOI: | 10.6342/NTU201601804 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學工程學系 |
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