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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 戴子安(Chi-An Dai) | |
dc.contributor.author | Xue-Yu Liu | en |
dc.contributor.author | 劉學昱 | zh_TW |
dc.date.accessioned | 2021-07-10T22:18:54Z | - |
dc.date.available | 2021-07-10T22:18:54Z | - |
dc.date.copyright | 2017-08-30 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-08-07 | |
dc.identifier.citation | 1. Hoffman, A.S., Hydrogels for biomedical applications. Advanced Drug Delivery Reviews, 2002. 54(1): p. 3-12.
2. Wichterle, O.L., D, nature, 1960. 185: p. 117-118. 3. takagi t., t.k., aizawa m., miyata s.,, in proceeding of the first international conference on intelligent materials. 1993, technomic: lanaster pa. 4. Hoffman, A.S., Hydrogels for biomedical applications. Advanced drug delivery reviews, 2012. 64: p. 18-23. 5. Shiroya, T., et al., Enzyme immobilization on thermosensitive hydrogel microspheres. Colloids and Surfaces B: Biointerfaces, 1995. 4(5): p. 267-274. 6. Takei, Y.G., et al., Temperature-responsive bioconjugates. 1. Synthesis of temperature-responsive oligomers with reactive end groups and their coupling to biomolecules. Bioconjugate chemistry, 1993. 4(1): p. 42-46. 7. Tanaka, T., et al., Collapse of Gels in an Electric Field. Science, 1982. 218(4571): p. 467. 8. Evans, D.F., et al., Measurement of gastrointestinal pH profiles in normal ambulant human subjects. Gut, 1988. 29(8): p. 1035. 9. Albin, G., T.A. Horbett, and B.D. Ratner, Glucose sensitive membranes for controlled delivery of insulin: Insulin transport studies. Journal of Controlled Release, 1985. 2: p. 153-164. 10. Tanaka, T., et al., Phase Transitions in Ionic Gels. Physical Review Letters, 1980. 45(20): p. 1636-1639. 11. Ataman, M., Properties of aqueous salt solutions of poly(ethylene oxide). Cloud points, θ temperatures. Colloid and Polymer Science, 1987. 265(1): p. 19-25. 12. Kjellander, R. and E. Florin, Water structure and changes in thermal stability of the system poly (ethylene oxide)–water. Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases, 1981. 77(9): p. 2053-2077. 13. Ricka, J. and T. Tanaka, Swelling of ionic gels: quantitative performance of the Donnan theory. Macromolecules, 1984. 17(12): p. 2916-2921. 14. Ishihara, K., N. Muramoto, and I. Shinohara, Controlled release of organic substances using polymer membrane with responsive function for amino compounds. Journal of Applied Polymer Science, 1984. 29(1): p. 211-217. 15. Ishihara, K. and I. Shinohara, Photoinduced permeation control of proteins using amphiphilic azoaromatic polymer membrane. Journal of Polymer Science: Polymer Letters Edition, 1984. 22(10): p. 515-518. 16. Eisenberg, S.R. and A.J. Grodzinsky, Electrically modulated membrane permeability. Journal of Membrane Science, 1984. 19(2): p. 173-194. 17. Bae, Y.H., T. Okano, and S.W. Kim, Temperature dependence of swelling of crosslinked poly(N,N′-alkyl substituted acrylamides) in water. Journal of Polymer Science Part B: Polymer Physics, 1990. 28(6): p. 923-936. 18. Escobedo, F.A. and J.J. de Pablo, Molecular simulation of polymeric networks and gels: phase behavior and swelling. Physics Reports, 1999. 318(3): p. 85-112. 19. Marchetti, M., S. Prager, and E. Cussler, Thermodynamic predictions of volume changes in temperature-sensitive gels. 1. Theory. Macromolecules, 1990. 23(6): p. 1760-1765. 20. Eichenbaum, G.M., et al., Investigation of the Swelling Response and Loading of Ionic Microgels with Drugs and Proteins: The Dependence on Cross-Link Density. Macromolecules, 1999. 32(15): p. 4867-4878. 21. Ratner, B.D., et al., Biomaterials science: an introduction to materials in medicine. 2004: Academic press. 22. Escobar-Chávez, J.J., et al., Applications of thermo-reversible pluronic F-127 gels in pharmaceutical formulations. Journal of Pharmacy & Pharmaceutical Sciences, 2006. 9(3): p. 339-58. 23. Lin, C.-C. and A.T. Metters, Hydrogels in controlled release formulations: Network design and mathematical modeling. Advanced Drug Delivery Reviews, 2006. 58(12–13): p. 1379-1408. 24. Amsden, B., Solute Diffusion within Hydrogels. Mechanisms and Models. Macromolecules, 1998. 31(23): p. 8382-8395. 25. Siepmann, J. and N.A. Peppas, Modeling of drug release from delivery systems based on hydroxypropyl methylcellulose (HPMC). Advanced Drug Delivery Reviews, 2012. 64, Supplement: p. 163-174. 26. Orakdogen, N. and O. Okay, Influence of the initiator system on the spatial inhomogeneity in acrylamide‐based hydrogels. Journal of Applied Polymer Science, 2007. 103(5): p. 3228-3237. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77738 | - |
dc.description.abstract | 本研究目的為開發一個可注射型之具有包覆藥物的水膠載體,並將其應用於藥物釋放系統上,利用注射式方式,注入含藥傷口修復膠,可避免開刀所產生之問題,經由數月之藥物釋放,肌腱之修復,與水膠之後續逐步降解,可成為完全不需手術之新式治療方法。
本實驗使用P407水膠作為可注射水膠之原型,P407為一種分別為親水-親油-親水三種鏈段的高分子,因此高分子水溶液會在低溫時與水均勻混合,而在高溫時達到LCST形成高分子水膠,但此水膠沒有緊密的機械強度,容易散掉,因此需要改變末端形成雙鍵以此化學交聯,形成高機械強度之高分子水膠。首先先將末端官能基清基(hydroxyl groups)經丙烯醯氯反應而形成具有丙烯酸官能基高分子,且經由核磁共振(NMR)確認丙烯酸有無接上。調整不同組成之P407DA與交聯劑EGDMA來改變水膠的孔洞結構,或是加入不同比例的acrylic acid來改變水膠與藥物之間的作用力,藉由改變這兩種因素進而影響藥物釋放的速率,由掃描釋電子顯微鏡(SEM)觀察孔洞大小的改變,並以動態熱機械分析儀(DMA)測量水膠之楊氏係數,輔以佐證孔洞大小的正確性,本實驗利用AA改變水膠對藥物的作用力,進而影響超音波對藥物釋放的作用速率,並且加入EGDMA改變水膠的porosity降低緩釋時的藥物釋放量以此達到類似週期性打針的目的。 | zh_TW |
dc.description.abstract | In this study, we designed an injectable and biodegradable hydrogel in drug delivery system. The drug can be injected with the hydrogel to make the drug keep at that place and avoid the problem of surgery. Through a few months of drug delivery, the hydrogel would degradable slowly becoming a new treatment without surgery
Hydrogels based on Poloxamer 407 marcromers are prepared by thermal-curing. Poloxamer 407 is triblock copolymers with PEO-PPO-PEO. So the Poloxamer will have the sol-gel transition. But the mechanical property of P407 is too low to maintain drug, we should make the P407 have double bound to form a chemical curing hydrogel. The terminal hydroxyl groups of marcromers are modified to acrylated functional groups, and further characterized by NMR to confirm acrylation. Different concentration of P407DA and crosslinker (EGDMA) change the porosity of hydrogel, and adding the acrylic acid will change the interaction of drug with hydrogel. By SEM, we can measurement the porosity of the hydrogel. Using DMA measurement the young’s modulus can double check the morphology of SEM. | en |
dc.description.provenance | Made available in DSpace on 2021-07-10T22:18:54Z (GMT). No. of bitstreams: 1 ntu-106-R04524073-1.pdf: 2106803 bytes, checksum: c0900650ef192499495f7f5c6bb87a49 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 致謝 I
中文摘要 II Abstract III List of Figure VII List of Table XI Chapter 1. Introduction 1 Chapter 2. Paper review 3 2-1 Hydrogel 3 2-1-1 Hydrogel for Engineering Application 3 2-1-2 Temperature Sensitive Polymer 5 2-1-3 Lower Critical Solution Temperature 6 2-1-4 Swelling Theory 7 2-1-5 Poly(ethylene glycol) (PEG) 9 2-1-6 Poloxamer 407 (P407) 10 2-2 Nucleophilic Acyl Substitution 13 2-3 Drug Delivery 16 2-3-1 Diffusion-Controlled of Drug Release 17 2-3-2 Swelling-Controlled of Drug Release 17 2-3-3 Chemically-Controlled of Drug Release 18 Chapter 3. Experimental 19 3-1 Materials and Equipments 19 N,N,N′,N′-Tetramethylethylenediamine 19 3-2 Synthesis of Diacrylate-Terminated Macromers by Nucleophilic Acyl Substitution 22 3-2-1 Synthesis of Poloxamer 407 diacrylate(P407DA) 22 3-3 Hydrogel Preparation 24 3-4 Characterization of Macromers and Hydrogel 28 3-4-1 1H Nuclear Magnetic Resonance (1H NMR) Characterization of Macromers 28 3-4-2 Equilibrium Swelling of hydrogel 28 3-4-3 Scanning Electron Microscopy (SEM) 29 3-4-4 Dynamic Mechanical Analyzer(DMA) 30 3-4-5 Rheometer 30 3-5 In Vitro Protein Release 31 3-5-1 Calibration Curve of Boveine Serum Albumin(BSA) 31 3-5-2 Hydrogel Drug Release 32 Chapter 4. Result and Discussion 33 4-1 1H Nuclear Magnetic Resonance (1H NMR) Spectrum of Macromers 33 4-2 Equilibrium Swelling Ratio of hydrogel 35 4-3 Scaning Electron Microscopy (SEM) 40 4-4 Dynamic Mechanical Analyzer (DMA) 44 4-5 Rheometer 49 4-6 In Vitro BSA Release 53 Chapter 5. Conclusion 59 Reference 61 | |
dc.language.iso | en | |
dc.title | 水膠開發及其藥物釋放應用 | zh_TW |
dc.title | Synthesis and Characterization of Hydrogels for Uses in Controlled-Release Application | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳文翔,謝宗霖,陳俊杉 | |
dc.subject.keyword | poloxamer 407,可注射水膠,藥物釋放,超音波, | zh_TW |
dc.subject.keyword | poloxamer 407,injectable hydrogel,drug delivery,ultrasonic, | en |
dc.relation.page | 63 | |
dc.identifier.doi | 10.6342/NTU201702690 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2017-08-07 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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