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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 羅翊禎(Yi-Chen Lo) | |
| dc.contributor.author | Yi-Shan Lai | en |
| dc.contributor.author | 賴宜姍 | zh_TW |
| dc.date.accessioned | 2021-07-10T21:42:44Z | - |
| dc.date.available | 2021-07-10T21:42:44Z | - |
| dc.date.copyright | 2020-08-10 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-07-30 | |
| dc.identifier.citation | 林欣誼. 以酵母菌發酵提高苦瓜甲醇萃取物中功效性成分3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al之探討. 國立臺灣大學生物資源暨農學院食品科技研究所碩士論文. 臺北, 臺灣. 2019. 邱群慧. 羅漢果皂苷分析與利用靈芝及酵母菌對其進行生物轉換. 國立臺灣大學生物資源暨農學院食品科技研究所碩士論文. 臺北, 臺灣. 2013. 崔竣杰; 李波; 程蛟文; 胡开林, 苦瓜苦味物质及其生物合成研究进展. 园艺学报 2015, 42 (9), 1707-1718. 莊莎莎. 羅漢果皂苷的製備與純化. 國立臺灣大學生物資源暨農學院食品科技研究所碩士論文. 臺北, 臺灣. 2016. 陳怡潔. 不同酵母菌珠對羅漢果皂苷轉換之影響. 國立臺灣大學生物資源暨農學院食品科技研究所碩士論文. 臺北, 臺灣. 2017. 劉宜叡. 加熱溫度及酵母菌生物轉換對苦瓜皂苷之影響. 國立臺灣大學生物資源暨農學院食品科技研究所碩士論文. 臺北, 臺灣. 2017. 賴韻如. 探討酵母菌與乳酸菌轉換苦瓜皂苷之影響. 國立臺灣大學生物資源暨農學院食品科技研究所碩士論文. 臺北, 臺灣. 2018. Abdel-Banat, B. M.; Nonklang, S.; Hoshida, H.; Akada, R., Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianus. Yeast 2010, 27 (1), 29-39. Ahn, Y. O.; Shimizu, B.-i.; Sakata, K.; Gantulga, D.; Zhou, Z.; Bevan, D. R.; Esen, A., Scopolin-hydrolyzing β-glucosidases in roots of Arabidopsis. Plant and Cell Physiology 2010, 51 (1), 132-143. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76993 | - |
| dc.description.abstract | 葡萄糖苷水解酶普遍存在於生物體中，可從多醣或醣苷的非還原端水解醣苷鍵，常被食品工業用來水解帶醣基的物質，提升產品的生理活性及改善風味。皂苷為植物的二級代謝產物，通常以醣苷形式存在，過去文獻提及水解醣苷上葡萄糖基能改善風味及提升皂苷的生理活性。先前實驗室利用多株酵母菌與乳酸菌篩選具有苦瓜皂苷能力之菌株，發現酵母菌Kluyveromyces marxianus具有轉換苦瓜中苦味皂苷Momordicoside L及Momordicoside K能力，同時提升具有降血糖功效成分3β, 7β, 25-trihydroxycucurbita-5, 23 (E)-dien-19-al之含量，並觀察到K.marxianus中β-glucosidase (Bgl1) 蛋白可能為轉換苦瓜皂苷之目標蛋白。本研究利用基因突變菌株更進一步驗證Bgl1為K.marxianus中主要轉換苦瓜皂苷之蛋白，並以Escherichia coli BL21 (DE3)大量表現Bgl1，利用親和性管柱純化，將純化蛋白質進行受質水解特異性探討，結果顯示從結構簡單的單環之硝基苯酚 (nitrophenyl)、二環之香豆素 (coumarins)衍生物及三環之黃酮類 (flavonoids)化合物，至結構較複雜的四環三萜類化合物苦瓜皂苷及羅漢果皂苷，Bgl1能水解不同環狀結構上O-linked醣基，也能水解羅漢果皂苷上β-1,6鍵結之醣鏈，但無法水解β-1,2鍵結之醣鏈。了解Bgl1在水解位點上具有特殊性，不同於文獻中指出β-glucosidase對於皂苷上O-linked糖基水解能力差之特性，顯示Bgl1未來可應用於水解不同苷元型態之植物功效性成分。 | zh_TW |
| dc.description.abstract | Saponins are secondary metabolites of plants, and commonly exist as glycosyl forms. Microbial biotransformation is a useful way to remove the glycosyl group for improving the flavor and producing the bioactive compounds in foods. Our previous studies indicated that yeasts and lactic acid bacteria had limited capacity to convert the bitter melon saponins. In contrast, yeast Kluyveromyces marxianus could be able to transform momordicoside L to antidiabetic compound 3β, 7β, 25 -trihydroxycucurbita-5,23(E)-dien-19-al (THC). Besides, it was observed that β-glucosidase (Bgl1) in K.marxianus was the putative protein for conversion. Here, we provide further evidence to demonstrate that Bgl1 is the target protein for bitter melon saponins transformation. We also characterize substrate specificity by purified Bgl1 protein. The results demonstrate that Bgl1 hydrolyzes broad spectrum of glycones, including coumarins derivative, flavonoids and tetracyclic triterpenes. In addition, this enzyme performs the hydrolysis on both the β-linked O-glycosyl bond of above stated glycosides and β-1,6 glycosidic bond in mogrosides. In conclusion, we characterized the function and substrate specificity of Bgl1 enzyme which may be valuable for bioactive compound production in the near future. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-10T21:42:44Z (GMT). No. of bitstreams: 1 U0001-2807202023203400.pdf: 4951635 bytes, checksum: 832934bd3917cd6e25b38d9ddb08f797 (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 目錄 第一章、 前言 1 第二章、 文獻回顧 2 第一節、酵母菌特性介紹 2 第二節、β-葡萄糖苷水解酶 3 一、Glycoside hydrolase family 3 (GH3) 4 二、酵母菌K.marxianus中的β-葡萄糖苷水解酶 6 第三節、生物轉換 7 一、香豆素衍生物 7 二、黃酮類化合物 8 三、羅漢果皂苷 8 四、苦瓜皂苷 9 第四節、酵素受質特異性(substrate specificity) 12 第三章、研究目的與實驗架構 15 第四章、材料與方法 17 第一節、實驗材料 17 一、山苦瓜 17 二、羅漢果皂苷萃取物 (Lo Han Kuo extracts, LHK extracts) 17 三、酵母菌菌株 17 四、質體 17 五、引子 17 六、藥品與試劑 21 第二節、儀器設備 23 一、實驗耗材 23 二、一般儀器設備 23 三、套裝軟體 24 第三節、實驗方法 25 一、菌株特性測試 25 二、酵母菌對皂苷之生物轉換 27 三、Bgl1蛋白表現及活性測試 35 第五章、結果與討論 42 第一節、K.marxianus細胞對苦瓜皂苷的轉換 42 一、K.marxianus細胞於苦瓜萃取物培養液中生長及存活狀況 42 二、不同培養液對於酵母菌外泌蛋白組成的影響 47 三、K.marxianus於苦瓜萃取物培養液中皂苷轉換能力 50 四、以S. cerevisiae系統表現Bgl1蛋白 52 五、以基因突變菌株轉換苦瓜皂苷 58 第二節、探討β-葡萄糖苷水解酶(Bgl1)水解特性 64 一、β-葡萄糖苷水解酶(Bgl1)之蛋白表現及活性 64 二、Bgl1蛋白之受質特異性 72 第六章、結論與展望 96 第七章、參考文獻 97 第八章、附錄 103 | |
| dc.language.iso | zh-TW | |
| dc.subject | O-linked醣基 | zh_TW |
| dc.subject | 苦瓜皂苷 | zh_TW |
| dc.subject | β-glucosidase | zh_TW |
| dc.subject | 受質水解特異性 | zh_TW |
| dc.subject | substrate specificity | en |
| dc.subject | β-linked O-glycosyl bond | en |
| dc.subject | bitter melon saponins | en |
| dc.subject | β-glucosidase | en |
| dc.title | 探討酵母菌中β-葡萄糖苷酶之特性及受質水解特異性 | zh_TW |
| dc.title | Characterization and substrate specificity of a yeast β-glucosidase | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 呂廷璋(Ting-Jang Lu),陳宏彰(Hong-Jhang Chen),陳勁初(Chin-Chu Chen),邱群惠(Chun-Hui Chiu),徐駿森(Chun-Hua Hsu) | |
| dc.subject.keyword | 苦瓜皂苷,β-glucosidase,受質水解特異性,O-linked醣基, | zh_TW |
| dc.subject.keyword | bitter melon saponins,β-glucosidase,substrate specificity,β-linked O-glycosyl bond, | en |
| dc.relation.page | 123 | |
| dc.identifier.doi | 10.6342/NTU202002004 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2020-07-30 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-2807202023203400.pdf 未授權公開取用 | 4.84 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。