探討添加幾丁聚醣於聚對苯二甲酸丁二酯不織布在血液過濾器上的應用

Abstract

血液中含有許來自不同細胞產生之細胞微小顆粒，相關文獻指出血液及血品中的細胞微小顆粒會引起輸血反應及輸血後的感染疾病。為了降低輸血治療造成的不良反應及疾病感染，去除治療血品中細胞微小顆粒含量是主要的目標。迄今，甚少文獻探討移除血品所產生之細胞微小顆粒，且目前臨床使用的血液過濾器也不具有除去細胞微小顆粒的功能。故本研究的目的在於開發出可去除細胞微小顆粒專用之過濾器，將造成輸血反應的因子濾除，有效減少輸血反應。本研究以PBT不織布，作為固定幾丁聚醣的基材，探討其固定後幾丁聚醣前後之差異，並以掃描式電子顯微鏡觀察PBT不織布其表面形貌，量測接觸角，並比較材料親疏水性的影響。由於不織布之高孔隙度，幾丁聚醣改質後增加親水性，提供吸附血中細胞微小顆粒的機會，我們利用儲存的血品釋放細胞微小顆粒，檢測血液中細胞微小顆粒的釋放及使用流式細胞儀對其分析，再利用雙重染色辨識細胞微小顆粒，並建立細胞微小顆粒計數方式，比較其過濾前及過濾後之差異，此結果可提供臨床上減少輸血相關副作用作為探討。

Blood contains many microparticles (MPs), also sometimes called microvesicles, derived from different cell types. Including platelets (PLTs), red blood cells(RBCs), white blood cells (WBCs), and endothelial cells in the circulation or during blood processing. Many papers have proven that transfusion reactions after transfusions are caused by microparticles in blood. In order to decrease above side effects, removal microparticles of blood products is the most important aspect. Few studies have addressed this problem and current filters used for blood filter are not designed to effectively remove microparticles. This study aims at designing a filter that can effectively remove these cellular microparticles and other cellar debris that are generated during blood storage. Established manufacturing method of modified PBT(polybutylenes terephthalate) non-woven for blood filtration. In order to improve the hydrophilicity and hemocompatibility of PBT fibers. PBT non-woven were treated with chitosan. The surface modified PBT fibers was analyzed by scanning electron microscopy, water contact angle. Chitosan was immobilized on the fabric fibers surfaces very homogeneously. The hydrophilicity of the composite PBT non-woven surface was improved. Overall results of this study demonstrated that the immobilization of chtiosan onto the surface of PBT fiber would be beneficial to improve the hydrophilicity and hemocompatibility.