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標題: | 各種壓力下水稻白葉枯病原菌RNA聚合?的修飾 Modification of RNA Polymerase from Xanthomonas campestris pv. oryzae under Various Types of Stresses |
作者: | 劉俊昇 |
出版年 : | 1992 |
學位: | 碩士 |
摘要: | 水稻白葉枯病原菌之RNA聚合?在其溶解性噬菌體Xp10感染後會遺失其σ次單位,為探討在其他四種壓力:靜止期,饑餓狀態,熱休克,冷休克下,RNA聚合?組成的變化及可能的修飾作用,乃純化出RNA聚合?σ及α次單位製備抗血清,在純化過程中發現,以本論文描述之條件純化所得RNA聚合?的組成?[α2ββ'σ],並未含有k次單位. 以抗核?血清免疫沉澱各種壓力下的RNA聚合?,並以抗次單位血清免疫染色發現,只有Xp10感染可使σ次單位自核醒上遺失,其他四種壓力下σ次單位仍與核?穩定結合,並無類似大腸桿菌的σ次單位置換作用.在這四種壓力下會分別誘發數種分子量介於13-22 kD的RNA聚合?連結蛋白質,惟其詳細功能仍未知.以雙向電泳分析Xp10感染後RNA聚合?可能的修飾作用,發現各次單位並未因Xp10的感染而產生共價性的修飾作用. 由本文結果發現,水稻白葉枯病原菌在壓力下的轉錄調節系統與研究較多的大腸桿菌系統有很大的不同,至於確實的調節系統為何?仍有待進一步研究. The σ subunit of RNA polymerase of Xanthomonas campestris pv. oryzae was lost during phage Xp10 infection. To study the compositional changes and modifications of RNA polymerase during Xp10 infection and other four types of stresses: stationary phase, starvation, heat shock, chilling shock, σ and a subunits were purified and antisera of these two subunits were prepared. The purified holoenzyme of RNA polymerase by method here emploied did not show k subunit. Results of immunopricipitation of RNA polymerase by anti-core antiserium and immunostaining with anti-subunits revealed that loss of the σ subunit occurred exclusively by Xp10 infection where as not in these four stresses. In addition, there was no detectable exchange of the σ subunit in these four stresses. Some RNA polymerase binding proteins with molecular weight between 13-22 kD were induced by these four stresses, but their functions deserve further study. No covalent modification in RNA polymerase during Xp10 infection could be found by 2-D electrophoresis. Results of this study suggest that the control of transcription in X. campestris pv. oryzae is different as used in E. coli during stress. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75894 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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