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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 劉俊昇 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:16:14Z | - |
| dc.date.available | 2021-07-01T08:16:14Z | - |
| dc.date.issued | 1992 | |
| dc.identifier.citation | Adhya, S. and S. Garges, 1990, Positive Control. J. Biol. Chem. 265: 10797-10800. Belfort, M. 1989, Bacteriophage Introns: Parasites within Parasites. Trends in Genet. 5:209-213. Chamberlin, J. M. 1976. RNA Polymerase. an overview in RNA Polymerase (Losick, R. and Chamberlin, M. eds), pp 17-67. Cold Spring Harbor Laboratory. Chamberlin, J. M., C. W. Nierman, J. Wiggs and N. Neff, 1979, Aquantitative assay for bacterial RNA polymerase. J. Biol. Chem. 254:10061-10069. Chen, C.K., J. To, and T. T. Kuo, 1989, A new subunit k for RNA polymerase from Xanthomonas campestris pv. oryzae. J. Biol. Chem. 264:4362-4366. Cozzone, A. J. 1988, Protein phosphorylation in prokaryotes. Ann. Rev. Microbiol. 42:97-125. Doi, R. H. and L. F. Wang, 1986, Multiple prokaryotic ribonucleic acid polymerase sigma factors. Microbiol. Reviews. 50:227-243. Guiduschek, E. P., T. Elliott, and G. A. Kassavetis, 1983. In Bacteriophage T4 (Berget, P., E. kutter, C. K. mathews, and G. Mosig, Eds) pp. 189-192. American Society of Microbiology, Washington D.C. Geiduschek, E. P., and G. A. kassavetis. 1988. Change in RNA polymerase. in The Bacteriolphages R. Calendar ed. Vol.1, pp. 93-115, Plenum Press, New York. Goff, C. G. 1974, Chemical structure of a modification of Escherichia coli ribonucleic acid polymerase a polypeptides induced by bacteriophage T4 infection. J. Biol. Chem. 249:6181-6190. Harlow, E. and D. lane, 1988. Antibodies: A laboratory manual. Cold Spring Harbor Laboratory. Helmann, J. D. and M. J. Chamberlin, 1988. Structure and function of bacterial sigma factors. Ann. Rev. Biochem. 57:355-387. Hesselbach, B. A. and D. Nakada, 1975. Inactive complex conformation between E. coli RNA polymerase and an inhibitor protein purified from T7 phage infected cells. Nature (London) 258:354-357. Hesselbach, B. A. and D. Nakada, 1976a. Host shutoff function of bacteriophage T7 involvement of T7 gene 2 and gene 0.7 in the inactivation of Eschericheria coli RNA polymerase. J. Virol. 24: 736-745. Hesselbach, B. A. and D. Nakada, 1976b, I protein: bacteriophage T7 coded inhibitor of Escherichia coli RNA polymerase. J. Virol. 24: 746-760. Liao, Y. D. and T. T. Kuo, 1986a. Los of σ factor of RNA polymerase of Xanthomonas campestris pv. oryzae during phage Xp10 infection. J. Biol. Chem. 261: 13714-13719 Liao, Y. D., J. To, T. Y. Feng, and T. T. Kuo. 1986b. Characterization of phage-Xp10-coded RNA polymerase. Eur. J. Biochem. 157: 571-577. Liao, Y. D., J. To, and T. T. Kuo. 1987, Regulation of transcription of Xp10 genome in bacteriophage-infected Xanthomonas. campestris pv. oryzae. J. Virol. 61: 1695-1699 McCarty, J. E. G., and C. Gualerzi. 1990, Translation control of prokaryote gene expression. Trends in Genet. 6: 78-85 McClure, W.R., 1985, Mechanism andcontrol of transcription initiation in prokaryotes. Ann. Rev. Biochem. 54: 171-204 Millipore, 1989, Protein blotting protocols for the immobilon-P transfer membrane. Millipore Corporation, Bedford, MA, USA. Neidhardt, F. C., R. A. VanBogelen, and V. Vaughn, 1894, The genetics and regulation of heat-shock proteins. Ann. Rev. Genet. 18: 295-329. O'Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007-4021. Rabussay, D. 1983, In Bacteriophage T4 (Berget, P., E. Kutter, C.K. Mathews, and G. Mosig, Eds) pp. 167-188. American Socity of Microbiology, Washington D.C. Reznikoff, W. S., D. A. Siegele, D. W. Cowing, and C. A. Gross, 1985, Structure and function of bacterial sigma factors. Ann. Rev. Biochem. 57: 839-872. Rohere, H., W. Zillig, and R. Mailhammer, 1975, ADP-ribosylation of DNA-dependent RNA polymerase of Escherichia coli by an NAD+: protein ADP-ribosyl-transferase from bacteriophage T4. Eur. J. Biochem. 60: 227-238. Sawadogo, M. 1990, RNA polymerase B (II) and general transcription factors. Ann. Rev. Biochem. 59: 711-754. Walker, J. M. 1984, Methods in molecular biology, Vol.1 Proteins. E. Shang. Yang, B. C., Y. F. Ho, J. S. Liu, and T. T. Kuo, 1992, Phosphorylation of proteins in Xanthomonas campestris pv. oryzae. Arch. Microbiol. in press. Yura, T. and A. Isihama, 1979, Genetics of bacterial RNA polymerase. Ann. Rev. Genet., 13: 59-97 Zillig, W., F. Fujiki, W. Blum, D. Janekovic, and M. Schweiger, 1975, In vivo and in vitro phosphorylation of DNA dependent RNA polymerase of Escherichia coli by bacteriophage-T7-inducedprotein kinase. Proc. Natl. Acad. Sci. USA. 72: 2506-2510. Zillig, W., P. Palm, and A. Heil, 1976, Function and reassembly of subunits of DNA-dependent RNA polymerase. in RNA Polymerase, R. Losick and M. Chamberlin (eds) pp. 101-125, Cold Spring Harbor Laboratory, Cold Spring Harbor. 莊榮輝，蘇仲卿，1987，蛋白質膠體電泳檢定法，電泳分離技術研討會論文集，曾義雄等主編，69頁至85頁，行政院國家科學委員會發行． 廖有地，1985，噬菌體Xp10基因轉錄的控制，國立臺灣大學植物研究所博士論文． 施麗珍，1981，噬菌體Xp10所誘導負責關閉寄主細胞轉錄的抑制劑，國立臺灣學植物研究所碩士論文． 陳景康，1987，水稻白葉枯病原菌RNA聚合?之組成，國立臺灣大學植物研究所碩士論文． 馬慧瑛，1991，噬菌體Xp10感染後寄主RNA聚合?及蛋白質合成之改變，國立臺灣大學植物研究所碩士論文． | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75894 | - |
| dc.description.abstract | 水稻白葉枯病原菌之RNA聚合?在其溶解性噬菌體Xp10感染後會遺失其σ次單位，為探討在其他四種壓力：靜止期，饑餓狀態，熱休克，冷休克下，RNA聚合?組成的變化及可能的修飾作用，乃純化出RNA聚合?σ及α次單位製備抗血清，在純化過程中發現，以本論文描述之條件純化所得RNA聚合?的組成?[α2ββ'σ]，並未含有k次單位． 以抗核?血清免疫沉澱各種壓力下的RNA聚合?，並以抗次單位血清免疫染色發現，只有Xp10感染可使σ次單位自核醒上遺失，其他四種壓力下σ次單位仍與核?穩定結合，並無類似大腸桿菌的σ次單位置換作用．在這四種壓力下會分別誘發數種分子量介於13-22 kD的RNA聚合?連結蛋白質，惟其詳細功能仍未知．以雙向電泳分析Xp10感染後RNA聚合?可能的修飾作用，發現各次單位並未因Xp10的感染而產生共價性的修飾作用． 由本文結果發現，水稻白葉枯病原菌在壓力下的轉錄調節系統與研究較多的大腸桿菌系統有很大的不同，至於確實的調節系統為何？仍有待進一步研究． | zh_TW |
| dc.description.abstract | The σ subunit of RNA polymerase of Xanthomonas campestris pv. oryzae was lost during phage Xp10 infection. To study the compositional changes and modifications of RNA polymerase during Xp10 infection and other four types of stresses: stationary phase, starvation, heat shock, chilling shock, σ and a subunits were purified and antisera of these two subunits were prepared. The purified holoenzyme of RNA polymerase by method here emploied did not show k subunit. Results of immunopricipitation of RNA polymerase by anti-core antiserium and immunostaining with anti-subunits revealed that loss of the σ subunit occurred exclusively by Xp10 infection where as not in these four stresses. In addition, there was no detectable exchange of the σ subunit in these four stresses. Some RNA polymerase binding proteins with molecular weight between 13-22 kD were induced by these four stresses, but their functions deserve further study. No covalent modification in RNA polymerase during Xp10 infection could be found by 2-D electrophoresis. Results of this study suggest that the control of transcription in X. campestris pv. oryzae is different as used in E. coli during stress. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:14Z (GMT). No. of bitstreams: 0 Previous issue date: 1992 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………………i 英文摘要……………………………………………………ii 簡寫對照表……………………………………………………iii 緒 言……………………………………………………1 材料與方法……………………………………………………5 結 果……………………………………………………23 討 論……………………………………………………28 圖 表……………………………………………………33 參考資料……………………………………………………46 | |
| dc.language.iso | zh-TW | |
| dc.title | 各種壓力下水稻白葉枯病原菌RNA聚合?的修飾 | zh_TW |
| dc.title | Modification of RNA Polymerase from Xanthomonas campestris pv. oryzae under Various Types of Stresses | en |
| dc.date.schoolyear | 80-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 49 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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