鎘對魚類細胞株和哺乳類細胞株之作用

Abstract

本實驗主要試驗了具有DNA切除修復能力（excision repair）的魚類細胞株TO-2對氯化鎘的敏感度，及鎘對DNA造成的傷害，和鎘是否會影響細胞對UV的敏感度。最後並檢查metallothionein在此細胞株的表現。在所有實驗並附帶比較哺乳類細胞株CHO-K1的反應。

TO-2 is a fish cell line derived from Tilapia ovary and is the only fish cell lin, we know now, that has excision repair capacity comparable to CHO-K1, a mammalian cell line. The effects of cadmium in mammalian cell lines have been investigated broadly. In this work, the effects of cadmium on DNA damages and UV sensitivities in TO-2 and CHO-K1 were surveyed. We found that the cadmium induced repariable DNA single strand breaks in TO-2 cells incubated with PBS but not in CHO-K1 cells. The UV sensitivities of two cell lines were significantly affected by cadmium pretreatment for 24 hours. However, it did not happened if the cadmium was added after UV irradiation. At last, the induction of metallothionein by cadmium in TO-2 cells were detected by cytoplasmic dot hybridization.