酵母RNA對雞血清蛋白及肌蛋白的影響

Abstract

本文試驗體外性及植物性RNA對於動物體內蛋白質合成的影響。所用的材料?酵母RNA及秀拔(Shaver)288只小公雞共130隻分分成五組：第一組?對照組，第二組注射0.9% Nacl溶液0.5c.c./100gm.體重，其餘三實驗組分別注射RNA 2，5，10mg/100gm體重。每週每組各檢驗雞三隻，以供測定其血清蛋白及肌蛋白。測血清蛋白用光電比色計，測定肌蛋白則用Kjeldahl裝置及其方法。 根據測定結果，各試驗組之血清蛋白及肌蛋白均有增加之趨勢，經與對照組相比較，注射2mg RNA/100g體重者，其肌蛋白增加6.36%其血清蛋白增加9.97%；注射5mg RNA/100g體重者，其肌蛋白增加8.37%，其血清蛋白增加4.32%；注射10mg RNA/100g體重者，其肌蛋白增加9.09%而其血清蛋白增加9.41%。 經用T檢定法測定，獲知各實驗組肌蛋白含量的增加?顯著，而血清蛋白的增加?不顯著。但酵母RNA對雞之血清蛋白至少有促進的趨勢。故植物性，體外性的RNA，在雞體內能參與蛋白質的合成作用。

The present study was conducted to determine the effects of yeast R N A on the serum protein and the muscular protein in chicken. 130 of 7-day-old Shaver 288# male chicken were allotted into 5 groups, 26 birds in each, by random. Group A was used as the initial control. Group B, the experimental control, was injected intramuscularly with 0.5ml of 0.9% Nacl Solution per 100 gm. body weight. The other three groups (C, D and E) were injected intramuscularly with 2, 5, and 10 mg. of yeast R N A per 100 gm. body weight. All of them were injected at 3-day interval. Each week, 3 birds from each group were sacrificed for determining the serum protein by Biuret method and the muscular protein by Kjeldahl method. The results of these experiments are as follows: 1. The experiment groups were compared with the initial control, birds injected with the doses of 2 mg.(C), 5 mg.(D) and 10 mg.(E) R N A were found that their muscular protein concentration were 6.36%, 8.3% and 9.09% higher than the control respectively, and that their serum protein concentration were 9.97%, 4.32% and 9.41% higher than the control too. 2. The difference between the experimental groups and the control determining by the t - test method, is significance in their muscular protein concentration, but the difference is not significance in their serum protein concentration. Yet, the yeast R N A actually has a tendency to increase the serum protein in chicken. The present results provide the direct evidence that the yeast R N A, one of the plant R N Aξ, can participate in the synthesis of protein, when it is injected intra-muscularly in chicken.