請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75522完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Shiu-Nan Chen | en |
| dc.contributor.author | 陳重仁 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:39Z | - |
| dc.date.available | 2021-07-01T08:13:39Z | - |
| dc.date.issued | 1985 | |
| dc.identifier.citation | 1. chen, S.N., Y. Ueno and G.H. Kou (1982). Proc. Natl. Counc. R.O.C. 6(1),93. 2. Chen, S.N., S.C. Chi, and G.H. Kou (1983 a). Fish Path. 18(1),14. 3 Chen, J.H., S.N. Chen, H.H. Shih and G.H. Kou (1983 b). Proceeding of ROC-Japan Cooperative Science Seminar on Fish Diseases 93. 4. Freshy, R.I. (1972). Lancet Sept. 2,488. 5. Aaroson, S.A. and G.J. Todaro (1968). Science 1, 62.1024. 6. Boyde, A., R.A. Weiss and P. Vesely (1972). Exp. Cell Res. 71,313. 7. Fisher, R.W. and T.W. Cooper (1976).J. Cell Biol. 34,569. 8. Hassel, J. and L. Engelhardt (1977). Exp. Cell Res. 1, 07, 159. 9. Holly, R.W. (1979). Proc. Natl. Acad. Sci. 75(2), 901. 10. Hutching, S.E. and G.H. Sato (1978). Proc. Natl. Acad. Sci. (75) 2, 107,901. 11. Pegrum, S.M. (1982). Exp. Cell Res. 138,147. 12. Poter, K.R., V. Fonte and G. Weiss (1974). Cancer Res. 34,1385 13. Kusyk, C.J. (1982) Gynecol. Oncol.14,324. 14. Atkin, N.B. (1979). Cancer 44,604. 15. Riet-Fox, M.F. (1979). Cancer 44,2108. 16. Toews, H.A. (1968). Obst. & Gyn. 32(4),465. 17. Ishihara, T. (1961). Can. Res. 22,375. 18. Wake, N. (1980). Can Res. 40,4512. 19. Pardee, A.B. (1974). Proc. Natl. Acad. Sci.71,1286. 20. Pardee, A.B. (1975). Proc. Natl. Acad. Sci. 72,4994. 21. Pardee, A.B. (1978). Ann. Rev. Biochem. 47,715. 22. Rossow, P.W. (1979). Proc. Natl. Acad. Sci. 76,4446. 23. Nicolson, G.L. (1976). Biochem. Biophys. Acta. 458,1. 24. Golstein, I.J. (1973). Adv. Carbohydrate Chem. Biochem. 35,127. 25. Sivak, A. and S.R. Wolman (1972). In Vitro 8,1. 26. Robbins, P.W., S.C. Hubbard, S.J. Turco, and D.F. Wirth (1977). Cell 12, 893. 27. Spiro, M.J., R.G. Spiro, and V.N. Bhoyroo (1979). J. Biol. Chem. 254,716. 28. Taylor, J. Herbert. (1984). Mol. Cell Biochem. 61,99. 29. Fainsod, A. (1984). Exp. Cell Res. 152,77. 30. Rao, P.N. (1984). J. Cell Physiol. 119,77. 31. Cocorane, C.C. (1982). Adv. Immunol. 33, 241. 32. Hunt, L.A., J.R. Etchinson, and D.F. Summers (1978). Proc. Natl. Acad. Sci. 75,754. 33. Tabas, I., S. Schlesinger, and Kornfeld (1978). J. Biol. Chem. 253,716. 34. Grimes, W.J. (1973). Biochemistry 12,990. 35. Hellstrom, K.E. (1978). Int. J. Cancer 21,317. 36. Furcht, L.T. (1979). Nature 227,393. 37. Venuta, S. and H. Rubin (1973). Proc. Natl. Acad. Sci. 70,653. 38. Ymada, K.M. and K. Older (1978).Nature 275,179. 39. Brady, R.O. and P.H. Fishman (1974). Biochem. Biophys. Acta. 355,121. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75522 | - |
| dc.description.abstract | The first In Vitro successfully established human ovarian cancer cell line in Taiwan area, designated OC-3/VGH was made from a patient with serious papillary adenocarcinoma of the ovary, using Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10-20% fetal calf serum. The cells were derived from patient’s tumor tissue and grew as a monolayer of epithloid cells in culture. The cells have a doubling time of 48 hours determined by using a haemacytometer. Giemsa banding of mataphase chromosomes had revealed that the distribution of chromosome number of OC-3/VGH varied widely from 38 to 68 with a model number of 62. The cells have been subcultured 97 passages since its initiation in June, 1983, and maintainted in active culture in Laboratory of Tumor Biology at Veterans General Hospital, Taipei. It is believed that the establishment of this cell line will provide an useful in vitro model for Gynecological neoplastims studies. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:39Z (GMT). No. of bitstreams: 0 Previous issue date: 1985 | en |
| dc.description.tableofcontents | Abstract…………2 Introduction……3 Materials & Methods………6-12 I. Materials……………………6-8 1. Tumor Tissue…………………6 2. Culture Media………………6 3. Hank’s Balanced Salt Solution (HBSS)……………6 4. Phosphate Buffer Solution…………………7 5. Trypsin /(EDTA) Solution…………………7 6. Giemsa Banding Solution…………………8 II. Methods…………………………8-12 1. Primary Culture……………………8 2. Subculture…………………………8 3. Growth Kinetics……………………9 4. Cell Storage in Frozen State……9 5. Reconstitution of Frozen Cells………10 6. Phase Contrast Microscopy…………10 7. Scanning Electron Microscopy………10 8. Tumorigenecity……………………11 9. Chromosome Study………………11 10. Bacteria Test………………12 11. Mycoplasma Test……………12 Results…………………13 Discussion…………………16 Figures………………20 Reference……………30 | |
| dc.language.iso | zh-TW | |
| dc.title | 人類卵巢癌瘤細胞株之試管培養及其特性之研究 | zh_TW |
| dc.date.schoolyear | 73-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 31 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。