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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Hsin-Ying Lin | en |
dc.contributor.author | 林欣穎 | zh_TW |
dc.date.accessioned | 2021-07-01T08:12:48Z | - |
dc.date.available | 2021-07-01T08:12:48Z | - |
dc.date.issued | 2002 | |
dc.identifier.citation | 丁懷謙 食藥用菇多醣體之免疫生理活性。食品工業第32卷第5期:28-42,2000。
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75352 | - |
dc.description.abstract | 本實驗分兩大部分:多醣體培養實驗及小白鼠體內抗氧化活性測試。 多醣體培養實驗探討裂褶菌深層醱酵培養多醣之濃度曲線並和傳統的靈芝多醣及最近備受注目的巴西蘑菇多醣做比較,希望能找出裂褶菌多醣的最適收成期間、最好的培養基及最佳培養方式。 小白鼠體內抗氧化活性測試是以培養好的裂褶菌多醣體以口服方式給予小白鼠,探討不同多醣濃度及不同使用期間對小白鼠抗氧化活性的影響。 在多醣的液態深層培養方面,裂褶菌比其他菌種容易生長,產生的多醣量也較多,通氣培養及適當攪拌培養液對多醣的產量都有幫助,而較適合裂褶菌多醣生產的為含3?4%葡萄糖、0.5%酵母抽出物,0.1% KH2PO4及0.05 % MgSO4的培養液,另外裂褶菌多醣經高溫高壓滅菌後,多醣仍存在,且多醣總醣量和滅菌前沒有差異。 在多醣對小白鼠的抗氧化活性測試方面,由肝臟及脾臟的SOD濃度變化趨勢都可看出多醣體的確可以使小白鼠體內的SOD濃度升高而達到抗氧化的效果。 | zh_TW |
dc.description.abstract | This study was divided into two parts: The first part was to study the in vitro culture of fungus to produce glucan. In this part we tried to use the different ways to culture three kinds of fungi included Schizophyllum commune, Ganoderma lucidum and Agaricus blazei. In fermentative culture, Schizophyllum commune was better than the other two fungi in growth rate and the rate of glucan production. The optimum cultural medium of the Schizophyllum commune included 3~4% of glucose, 0.5% of yeast extract , 0.1% of KH2PO4 and 0.05% of MgSO4, and optimum stirring and aeration will increase glucan production in liquid culture. There was no change when the schizophyllan were treated with high temperature and high pressure. In the second part of this study we wanted to know the influence of SOD concentration when the mice were fed with glucan in different concentration and different feeding days. We finded when schizophyllan were added into drinking water, the SOD concentration in liver and spleen of mice will increase . | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2002 | en |
dc.description.tableofcontents | 一. 前言………………………………………………………………………………1 1.1 研究背景………………………………………………………………………1 1.2 菇蕈多醣體的結構……………………………………………………………2 1.3 菇蕈多醣體所引起的主要生理活性…………………………………………3 1.3.1 抗腫瘤作用………………………………………………………………3 1.3.2 免疫保護作用……………………………………………………………4 1.3.3 降血糖作用………………………………………………………………5 1.3.4 抗自由基…………………………………………………………………5 1.4 菇蕈多醣體之深層醱酵培養…………………………………………………8 1.4.1 深層醱酵培養及傳統固態培養的比較…………………………………8 1.4.2 深層醱酵培養的定義及培養基組成……………………………………8 1.4.3 影響深層醱酵培養的其他因數…………………………………………10 1.5 實驗中所使用到之菌種介紹…………………………………………………12 1.5.1 靈芝………………………………………………………………………12 1.5.2 巴西蘑菇…………………………………………………………………13 1.5.3 裂褶菌……………………………………………………………………15 1.6 菇蕈多醣體未來的發展………………………………………………………17 二.研究目的…………………………………………………………………………18 三.材料與方法………………………………………………………………………19 A.多醣體培養實驗…………………………………………………………………19 (一)多醣體的醱酵培養……………………………………………………………19 (二)多醣含量測定…………………………………………………………………20 (三)比較不同來源多醣體的濃度曲線……………………………………………22 (四)攪拌程度對裂褶菌多醣產量的影響…………………………………………22 (五)通氣對裂褶菌多醣濃度曲線的影響…………………………………………23 (六)碳源濃度對裂褶菌多醣濃度曲線的影響……………………………………23 (七)其他添加物對裂褶菌多醣產量的影響………………………………………23 (八)高溫高壓對多醣量的影響……………………………………………………25 B.小白鼠體內抗氧化活性測試……………………………………………………25 (一)實驗分組及處理方法…………………………………………………………25 (二)多醣飲料的處理………………………………………………………………26 (三)血液及內臟樣本處理方法……………………………………………………26 (四)測量內臟中蛋白質含量………………………………………………………27 (五)測量內臟中 SOD含量…………………………………………………………28 (六)實驗數據統計方法……………………………………………………………30 四.實驗結果…………………………………………………………………………31 A.多醣體培養實驗…………………………………………………………………31 (一)多醣體的醱酵培養……………………………………………………………31 (二)多醣含量測定…………………………………………………………………32 (三)比較不同來源多醣體的濃度曲線……………………………………………32 (四)攪拌程度對裂褶菌多醣產量的影響…………………………………………33 (五)通氣有無對裂褶菌多醣濃度曲線的影響……………………………………33 (六)碳源濃度對裂褶菌多醣濃度曲線的影響……………………………………34 (七)其他添加物對裂褶菌多醣產量的影響………………………………………35 (八)高溫高壓對多醣量的影響……………………………………………………35 B.小白鼠體內抗氧化活性測試……………………………………………………36 (一)實驗期間小白鼠的體重變化…………………………………………………36 (二)實驗期間小白鼠的食物消耗量………………………………………………36 (三)實驗期間小白鼠的飲水量……………………………………………………37 (四)斯實驗期問小白鼠的多醣攝取量……………………………………………37 (五)小白鼠的肝臟SOD變化 ………………………………………………………38 (六)小白鼠的脾臟SOD變化 ………………………………………………………38 五.討論………………………………………………………………………………39 A.多醣體培養實驗…………………………………………………………………39 (一)多醣體的醱酵培養……………………………………………………………39 (二)多醣含量測定…………………………………………………………………40 (三)比較不同來源多醣體的濃度曲線……………………………………………41 (四)攪拌程度對裂褶菌多醣產量的影響…………………………………………42 (五)通氣有無對裂褶菌多醣濃度曲線的影響……………………………………43 (六)碳源濃度對裂褶菌多醣濃度曲線的影響……………………………………44 (七)其他添加物對裂褶菌多醣產量的影響………………………………………45 (八)高溫高壓對多醣量的影響……………………………………………………46 B.小白鼠體內抗氧化活性測試……………………………………………………47 (一)實驗期間小白鼠的體重變化…………………………………………………47 (二)實驗期間小白鼠的食物消耗量………………………………………………48 (三)實驗期間小白鼠的飲水量……………………………………………………48 (四)小白鼠的肝臟 SOD變化………………………………………………………49 (五)小白鼠的脾臟 SOD變化………………………………………………………51 C.結論………………………………………………………………………………52 D.建議及應該改進之處……………………………………………………………53 六.參考文獻…………………………………………………………………………54 | |
dc.language.iso | zh-TW | |
dc.title | 裂褶菌多醣體的濃度分析及其對小白鼠之抗氧化活性測試 | zh_TW |
dc.title | The Concentration Analysis of Schizophyllan and The Measurement of Antioxidative Effect on Mice | en |
dc.date.schoolyear | 90-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 107 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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