評估以石蓴水解物生產生質乙醇

Abstract

As the effects of climate change continues to plague the world as a result of our continual use of one of the chief contributor’s ‘fossil fuels’, an alternative to this source of fuel should be developed. Bioethanol is the ideal substitute as it is made up from the fermentation of organic substrates. In this study, Ulva lactuca was used as a feedstock for bioethanol production using a combination of acidic pretreatment and enzymatic hydrolysis; followed by fermentation strategies to determine the effects of agitation, addition of nitrogen sources, substrate loading, temperature, pH on monocultures using Kluyveromyces marxianus K-21 (K-21) and Pichia stipitis BCRC 21777 (BCRC 21777) and a co-culture. The results of the agitation experiments revealed that static culture produced slightly higher ethanol (7.8±0.4 g/L) than agitations at 50 rpm and 150 rpm (6.10±0.7 g/L and 5.90±0.23 g/L) for K-21 and static culture, 50, 150 rpm for Pichia stipitis was 5.21±0.37 g/L, 4.90±0.19 g/L, and 4.80±0.35 g/L respectively. 10% substrate loading was found to be the optimum for both yeast K-21 and BCRC 21777 with ethanol concentrations of 10.60 ± 0.31 g/L and 6.8±0.2 g/L was obtained at optimized fermentation conditions of 35oC, static culture and a pH of 6. For the result of the co-culture a total amount of 11.5±0.7 g/L bioethanol was produced. The results of this research proposes that Ulva lactuca could be used as a feedstock for bioethanol production.