請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72982
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 呂廷璋(Ting-Jang Lu) | |
dc.contributor.author | Ya-Wen Li | en |
dc.contributor.author | 李雅雯 | zh_TW |
dc.date.accessioned | 2021-06-17T07:12:41Z | - |
dc.date.available | 2026-01-18 | |
dc.date.copyright | 2021-03-08 | |
dc.date.issued | 2021 | |
dc.date.submitted | 2021-01-18 | |
dc.identifier.citation | 何玟穎; 陳垣志; 陳凌哲; 廖寶琦 ( 2018 )。高解析質譜技術在分析新興濫用藥物的應用與展望。台灣法醫學誌, 10, 26-37。 馬成俊 ( 2005 )。皂苷及抗腫瘤構效關係的研究。中國科學院海洋研究所博士班畢業論文。 陳文惠; 劉芳淑; 羅吉方; 林哲輝 ( 2010 )。市售人參及西洋參藥材之鑑別。食品藥物研究年報 , 1: 289-298。 Baeg, I.-H.; So, S.-H., The world ginseng market and the ginseng (Korea). J. Ginseng Res. 2013, 37, 1-7. Balsevich, J. J.; Bishop, G. G.; Deibert, L. K., Use of digitoxin and digoxin as internal standards in HPLC analysis of triterpene saponin‐containing extracts. Phytochem. Anal. 2009, 20, 38-49. Brown, P. N.; Lister, P., Current initiatives for the validation of analytical methods for botanicals. Curr. Opin. Biotechnol. 2014, 25, 124-128. Bui Thanh, T.; Hải, N., Phytochemical and pharmacology effect of Panax notoginseng. J. Appl. Pharm. Sci. 2016, 6, 174-178. Chen, C.-f.; Chiou, W.-f.; Zhang, J.-t., Comparison of the pharmacological effects of Panax ginseng and Panax quinquefolium. Acta Pharmacol. Sin. 2008, 29, 1103. Chen, J.; Guo, X.; Song, Y.; Zhao, M.; Tu, P.; Jiang, Y., MRM-based strategy for the homolog-focused detection of minor ginsenosides from notoginseng total saponins by ultra-performance liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry. RSC Adv. 2016, 6, 96376-96388. Chen, J.; Tan, M.; Zou, L.; Liu, X.; Chen, S.; Shi, J.; Chen, C.; Wang, C.; Mei, Y., Qualitative and Quantitative Analysis of the Saponins in Panacis Japonici Rhizoma Using Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole-Time of Flight Tandem Mass Spectrometry and Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole-Linear Ion Trap Tandem Mass Spectrometry. Chem. Pharm. Bull. 2019, 67, 839-848. Chen, Y.; Zhao, Z.; Chen, H.; Yi, T.; Qin, M.; Liang, Z., Chemical Differentiation and Quality Evaluation of Commercial Asian and American Ginsengs based on a UHPLC–QTOF/MS/MS Metabolomics Approach. Phytochem. Anal. 2015, 26, 145-160. Cho, J. G.; Lee, M. K.; Lee, J. W.; Park, H. J.; Lee, D. Y.; Lee, Y. H.; Yang, D. C.; Baek, N. I., Physicochemical characterization and NMR assignments of ginsenosides Rb1, Rb2, Rc, and Rd isolated from Panax ginseng. J. Ginseng Res. 2010, 34, 113-121. Chu, C.; Xu, S.; Li, X.; Yan, J.; Liu, L., Profiling the Ginsenosides of Three Ginseng Products by Lc-Q-Tof/Ms. J. Food Sci. 2013, 78, C653-C659. Cui, J. F.; Garle, M.; Lund, E.; Bjorkhem, I.; Eneroth, P., Analysis of Ginsenosides by Chromatography and Mass Spectrometry: Release of 20 S-Protopanaxadiol and 20 S-Protopanaxatriol for Quantitation. Anal. Biochem. 1993, 210, 411-417. Domon, B.; Costello, C. E., A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates. Glycoconjugate J. 1988, 5, 397-409. Donno, D.; Boggia, R.; Zunin, P.; Cerutti, A. K.; Guido, M.; Mellano, M. G.; Prgomet, Z.; Beccaro, G. L., Phytochemical fingerprint and chemometrics for natural food preparation pattern recognition: an innovative technique in food supplement quality control. J. Food Sci. Technol. 2016, 53, 1071-1083. Du, H.; Lai, L.; Wang, F.; Sun, W.; Zhang, L.; Li, X.; Wang, L.; Jiang, L.; Zheng, Y., Characterisation of flower colouration in 30 Rhododendron species via anthocyanin and flavonol identification and quantitative traits. Plant Biol. 2018a, 20, 121-129. Du, Z.; Li, J.; Zhang, X.; Pei, J.; Huang, L., An Integrated LC-MS-Based Strategy for the Quality Assessment and Discrimination of Three Panax Species. Molecules 2018b, 23, 2988. Fuzzati, N., Analysis methods of ginsenosides. J. Chromatogr. B 2004, 812, 119-133. Guo, J.; Song, L.; Lv, Z.; Mou, X.; Qiao, R.; Zhu, X., Rapid determination of ginsenosides in health food and its application. J. Food Saf. Qual. 2019, 10, 117-122. Huang, X.; Liu, Y.; Zhang, N.; Sun, X.; Yue, H.; Chen, C.; Liu, S., UPLC Orbitrap HRMS Analysis of Panax quinquefolium L. for Authentication of Panax Genus with Chemometric Methods. J. Chromatogr. Sci. 2017, 56, 25-35. Huang, X.; Liu, Y.; Zhang, Y.; Li, S.-P.; Yue, H.; Chen, C.-B.; Liu, S.-Y., Multicomponent assessment and ginsenoside conversions of Panax quinquefolium L. roots before and after steaming by HPLC-MS(n). J. Ginseng Res. 2019, 43, 27-37. Jang, G. Y.; Kim, M. Y.; Lee, Y. J.; Li, M.; Shin, Y. S.; Lee, J.; Jeong, H. S., Influence of organic acids and heat treatment on ginsenoside conversion. J. Ginseng Res. 2018, 42, 532-539. Jia, L.; Zuo, T.; Zhang, C.; Li, W.; Wang, H.; Hu, Y.; Wang, X.; Qian, Y.; Yang, W.; Yu, H., Simultaneous Profiling and Holistic Comparison of the Metabolomes among the Flower Buds of Panax ginseng, Panax quinquefolius, and Panax notoginseng by UHPLC/IM-QTOF-HDMS(E)-Based Metabolomics Analysis. Molecules 2019, 24, 2188. Kang, K. S.; Kim, H. Y.; Yamabe, N.; Yokozawa, T., Stereospecificity in hydroxyl radical scavenging activities of four ginsenosides produced by heat processing. Bioorg. Med. Chem. Lett. 2006, 16, 5028-5031. Kim, D.-H., Chemical Diversity of Panax ginseng, Panax quinquifolium, and Panax notoginseng. J. Ginseng Res. 2012, 36, 1-15. Kim, H.-K., Pharmacokinetics of ginsenoside Rb1 and its metabolite compound K after oral administration of Korean Red Ginseng extract. J. Ginseng Res. 2013, 37, 451. Kim, M.-S.; Jeon, S.-J.; Youn, S. J.; Lee, H.; Park, Y.-J.; Kim, D.-O.; Kim, B.-Y.; Kim, W.; Baik, M.-Y., Enhancement of Minor Ginsenosides Contents and Antioxidant Capacity of American and Canadian Ginsengs (Panax quinquefolius) by Puffing. Antioxidants 2019, 8, 527. Kim, M. H.; Lee, Y. C.; Choi, S. Y.; Cho, C.-W.; Rho, J.; Lee, K.-W., The changes of ginsenoside patterns in red ginseng processed by organic Acid impregnation pretreatment. J. Ginseng Res. 2011, 35, 497-503. Kim, S.-J.; Murthy, H. N.; Hahn, E.-J.; Lee, H. L.; Paek, K.-Y., Parameters affecting the extraction of ginsenosides from the adventitious roots of ginseng (Panax ginseng C.A. Meyer). Sep. Purif. Technol. 2007, 56, 401-406. Kim, Y.-J.; Zhang, D.; Yang, D.-C., Biosynthesis and biotechnological production of ginsenosides. Biotechnol. Adv. 2015, 33, 717-735. Kim, Y.-R.; Yang, C.-S., Protective roles of ginseng against bacterial infection. Microbial Cell 2018, 5, 472. Lee, J. W.; Choi, B.-R.; Kim, Y.-C.; Choi, D. J.; Lee, Y.-S.; Kim, G.-S.; Baek, N.-I.; Kim, S.-Y.; Lee, D. Y., Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS. Molecules 2017a, 22, 2147. Lee, J. W.; Ji, S.-H.; Lee, Y.-S.; Choi, D. J.; Choi, B.-R.; Kim, G.-S.; Baek, N.-I.; Lee, D. Y., Mass Spectrometry Based Profiling and Imaging of Various Ginsenosides from Panax ginseng Roots at Different Ages. Int. J. Mol. Sci. 2017b, 18, 1114. Lee, S. M., Thermal conversion pathways of ginsenoside in red ginseng processing. Nat Prod Sci 2014, 20, 119-125. Leung, K. W.; Wong, A. S.-T., Pharmacology of ginsenosides: a literature review. Chin. Med. 2010, 5, 20. Li, L.; Luo, G.-A.; Liang, Q.-L.; Hu, P.; Wang, Y.-M., Rapid qualitative and quantitative analyses of Asian ginseng in adulterated American ginseng preparations by UPLC/Q-TOF-MS. J. Pharm. Biomed. Anal. 2010a, 52, 66-72. Li, L.; Wang, Y.; Xiu, Y.; Liu, S., Chemical Differentiation and Quantitative Analysis of Different Types of Panax Genus Stem-Leaf Based on a UPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis Approach. J. Anal. Methods Chem. 2018, 2018, 9598672-9598672. Li, S.-L.; Lai, S.-F.; Song, J.-Z.; Qiao, C.-F.; Liu, X.; Zhou, Y.; Cai, H.; Cai, B.-C.; Xu, H.-X., Decocting-induced chemical transformations and global quality of Du–Shen–Tang, the decoction of ginseng evaluated by UPLC–Q-TOF-MS/MS based chemical profiling approach. J. Pharm. Biomed. Anal. 2010b, 53, 946-957. Ligor, T.; Ludwiczuk, A.; Wolski, T.; Buszewski, B., Isolation and determination of ginsenosides in American ginseng leaves and root extracts by LC-MS. Anal. Bioanal. Chem. 2005, 383, 1098-1105. Lin, H.; Zhu, H.; Tan, J.; Wang, C.; Dong, Q.; Wu, F.; Wang, H.; Liu, J.; Li, P.; Liu, J., Comprehensive Investigation on Metabolites of Wild-Simulated American Ginseng Root Based on Ultra-High-Performance Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry. J. Agric. Food Chem. 2019, 67, 5801-5819. Liu, F.; Ma, N.; He, C.; Hu, Y.; Li, P.; Chen, M.; Su, H.; Wan, J.-B., Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detector. J. Ginseng Res. 2018, 42, 149-157. Liu, H.; Yang, J.; Yang, W.; Hu, S.; Wu, Y.; Zhao, B.; Hu, H.; Du, S., Focus on Notoginsenoside R1 in Metabolism and Prevention Against Human Diseases. Drug Des. Dev. Ther. 2020, 14, 551. Liu, J.; Liu, Y.; Zhao, L.; Zhang, Z.-H.; Tang, Z.-H., Profiling of ginsenosides in the two medicinal Panax herbs based on ultra-performance liquid chromatography-electrospray ionization–mass spectrometry. SpringerPlus 2016, 5, 1770. Liu, L.; Zhu, X.-M.; Wang, Q.-J.; Zhang, D.-L.; Fang, Z.-M.; Wang, C.-Y.; Wang, Z.; Sun, B.-S.; Wu, H.; Sung, C.-K., Enzymatic preparation of 20(S, R)-protopanaxadiol by transformation of 20(S, R)-Rg3 from black ginseng. Phytochemistry 2010, 71, 1514-1520. Liu, Z.; Li, Y.; Li, X.; Ruan, C.-C.; Wang, L.-J.; Sun, G.-Z., The effects of dynamic changes of malonyl ginsenosides on evaluation and quality control of Panax ginseng C.A. Meyer. J. Pharm. Biomed. Anal. 2012, 64-65, 56-63. Ma, L.-Y.; Zhang, Y.-B.; Zhou, Q.-L.; Yang, Y.-F.; Yang, X.-W., Simultaneous determination of eight ginsenosides in rat plasma by liquid chromatography–electrospray ionization tandem mass spectrometry: Application to their pharmacokinetics. Molecules 2015, 20, 21597-21608. Noh, K.-H.; Oh, D.-K., Production of the rare ginsenosides compound K, compound Y, and compound Mc by a thermostable β-glycosidase from Sulfolobus acidocaldarius. Biol. Pharm. Bull. 2009, 32, 1830-1835. Nozimakhonim, Y. Optimization of analytical method for the quality control of ginseng preparations and comparative analysisof ginsenosides of Wild Panax species using LC-ELSD and LC-Q-TOF-MS. Doctoral dissertation, Seoul National University, 2015. Park, H.-W.; In, G.; Han, S.-T.; Lee, M.-W.; Kim, S.-Y.; Kim, K.-T.; Cho, B.-G.; Han, G.-H.; Chang, I.-M., Simultaneous determination of 30 ginsenosides in Panax ginseng preparations using ultra performance liquid chromatography. J. Ginseng Res. 2013, 37, 457-467. Park, H.-W.; In, G.; Kim, J.-H.; Cho, B.-G.; Han, G.-H.; Chang, I.-M., Metabolomic approach for discrimination of processed ginseng genus (Panax ginseng and Panax quinquefolius) using UPLC-QTOF MS. J. Ginseng Res. 2014, 38, 59-65. Park JH. Multi-platform metabolomics approach to evaluate the authenticity of herbal medicine. Doctoral dissertation, Seoul National University, 2017. Pavia, D. L.; Lampman, G. M.; Kriz, G. S.; Vyvyan, J. A., Introduction to spectroscopy. Cengage Learning: 2008. Peng, M.; Yi, Y. X.; Zhang, T.; Ding, Y.; Le, J., Stereoisomers of Saponins in Panax notoginseng (Sanqi): A Review. Front. Pharmacol. 2018, 9, 188-188. Qi, L.-W.; Wang, C.-Z.; Yuan, C.-S., American ginseng: Potential structure–function relationship in cancer chemoprevention. Biochem. Pharmacol. 2010, 80, 947-954. Qi, L.-W.; Wang, C.-Z.; Yuan, C.-S., Ginsenosides from American ginseng: Chemical and pharmacological diversity. Phytochemistry 2011a, 72, 689-699. Qi, L.-W.; Wang, C.-Z.; Yuan, C.-S., Isolation and analysis of ginseng: advances and challenges. Nat. Prod. Rep. 2011b, 28, 467-495. Qiao, X.; Ye, M.; Liang, Y.-H.; Yang, W.-Z.; Guo, D.-A., Retention behaviors of natural products in reversed-phase liquid chromatography using mobile phase comprising methanol, acetonitrile and water. J. Sep. Sci. 2011, 34, 169-175. Quan, K.; Liu, Q.; Wan, J.-Y.; Zhao, Y.-J.; Guo, R.-Z.; Alolga, R. N.; Li, P.; Qi, L.-W., Rapid preparation of rare ginsenosides by acid transformation and their structure-activity relationships against cancer cells. Sci Rep 2015, 5, 8598. Rauniyar, N., Parallel Reaction Monitoring: A Targeted Experiment Performed Using High Resolution and High Mass Accuracy Mass Spectrometry. Int. J. Mol. Sci. 2015, 16, 28566-28581. Sang Min, J.; Jun-Ho, L.; Jong-Hoon, K.; Byung-Hwan, L.; In-Soo, Y.; Joon-Hee, L.; Dong-Hyun, K.; Hyewhon, R.; Yangmee, K.; Seung-Yeol, N., Stereospecificity of Ginsenoside Rg3 Action on Ion Channels. Mol. Cells 2004, 18, 383-389. Shi, X.-j.; Yang, W.-z.; Qiu, S.; Yao, C.-l.; Shen, Y.; Pan, H.-q.; Bi, Q.-r.; Yang, M.; Wu, W.-y.; Guo, D.-a., An in-source multiple collision-neutral loss filtering based nontargeted metabolomics approach for the comprehensive analysis of malonyl-ginsenosides from Panax ginseng, P. quinquefolius, and P. notoginseng. Anal. Chim. Acta 2017, 952, 59-70. Siddiqi, M. Z.; Jin, M.; Song, B.-K.; Park, H. Y.; Oh, J.-M.; Chun, S.; Jin, F.; Yu, H.; Im, W.-T., Enhanced Production of Protopanaxatriol from Ginsenoside Re and Rg1 Using a Recombinant Bacterial β-glucosidase. Biotechnol. Bioprocess Eng. 2019, 24, 632-637. Song, F.; Liu, Z.; Liu, S.; Cai, Z., Differentiation and identification of ginsenoside isomers by electrospray ionization tandem mass spectrometry. Anal. Chim. Acta 2005, 531, 69-77. Sun, B.-S.; Xu, M.-Y.; Li, Z.; Wang, Y.-B.; Sung, C.-K., UPLC-Q-TOF-MS/MS Analysis for Steaming Times-dependent Profiling of Steamed Panax quinquefolius and Its Ginsenosides Transformations Induced by Repetitious Steaming. J. Ginseng Res. 2012, 36, 277-290. Sun, T.-T.; Liang, X.-L.; Zhu, H.-Y.; Peng, X.-L.; Guo, X.-J.; Zhao, L.-S., Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MS. J. Ginseng Res. 2016, 40, 220-228. Tanaka, K.; Kubota, M.; Zhu, S.; Sankawa, U.; Komatsu, K., Analysis of Ginsenosides in Ginseng Drugs Using Liquid Chromatography-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Nat. Prod. Commun. 2007, 2, 1934578X0700200602. Wan, J.-Y.; Wang, C.-Z.; Liu, Z.; Zhang, Q.-H.; Musch, M. W.; Bissonnette, M.; Chang, E. B.; Li, P.; Qi, L.-W.; Yuan, C.-S., Determination of American ginseng saponins and their metabolites in human plasma, urine and feces samples by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. J. Chromatogr. B 2016, 1015-1016, 62-73. Wang, H.-P.; Zhang, Y.-B.; Yang, X.-W.; Zhao, D.-Q.; Wang, Y.-P., Rapid characterization of ginsenosides in the roots and rhizomes of Panax ginseng by UPLC-DAD-QTOF-MS/MS and simultaneous determination of 19 ginsenosides by HPLC-ESI-MS. J. Ginseng Res. 2016, 40, 382-394. Wang, L.; Yang, X.; Yu, X.; Yao, Y.; Ren, G., Evaluation of Antibacterial and Anti-inflammatory Activities of Less Polar Ginsenosides Produced from Polar Ginsenosides by Heat-transformation. J. Agric. Food Chem. 2013, 61, 12274-12282. Wen-Juan, M.; Ying, H.; Li, J.; ZHANG, C.-X.; Wen-Zhi, Y.; ZHANG, P.; De-An, G., Profiling and identification of chemical components of Shenshao Tablet and its absorbed components in rats by comprehensive HPLC/DAD/ESI-MSn analysis. Chin. J. Nat. Med. 2018, 16, 791-800. Wu, H.; Liu, H.; Bai, J.; Lu, Y.; Du, S., Simultaneous determination of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and 20(S) protopanaxatriol in beagle dog plasma by ultra high performance liquid mass spectrometry after oral administration of a Panax notoginseng saponin preparation. J. Chromatogr. B 2015, 974, 42-47. Xia, Y.-G.; Song, Y.; Liang, J.; Guo, X.-D.; Yang, B.-Y.; Kuang, H.-X., Quality Analysis of American Ginseng Cultivated in Heilongjiang Using UPLC-ESI(-)-MRM-MS with Chemometric Methods. Molecules (Basel, Switzerland) 2018, 23, 2396. Xie, F.; Li, S.; Cheng, Z.; Liu, X.; Zhang, H.; Li, P.; Liu, Z.; Guo, X.; Yu, P., Determination of 20(S)-protopanaxadiol in human plasma by HPLC–MS/MS: application to a pharmacokinetic study. Acta Pharm. Sin. B 2013, 3, 385-391. Xie, G. X.; Ni, Y.; Su, M. M.; Zhang, Y. Y.; Zhao, A. H.; Gao, X. F.; Liu, Z.; Xiao, P. G.; Jia, W., Application of ultra-performance LC-TOF MS metabolite profiling techniques to the analysis of medicinal Panax herbs. Metabolomics 2008, 4, 248-260. Xiu, Y.; Li, X.; Sun, X.; Xiao, D.; Miao, R.; Zhao, H.; Liu, S., Simultaneous determination and difference evaluation of 14 ginsenosides in Panax ginseng roots cultivated in different areas and ages by high-performance liquid chromatography coupled with triple quadrupole mass spectrometer in the multiple reaction–monitoring mode combined with multivariate statistical analysis. J. Ginseng Res. 2019, 43, 508-516. Xu, J.-D.; Wu, J.; Zhou, S.-S.; Shen, H.; Mao, Q.; Zhu, H.; Kong, M.; Li, S.-L., High performance liquid chromatography–electrospray ionization-mass spectrometry with programmed ionization mode switching and time segment scanning approach for quantifying multi-components in traditional complex herbal medicines, Qiong-Yu-Gao as an example. J. Pharm. Biomed. Anal. 2015, 112, 139-146. Xu, X.-f.; Cheng, X.-l.; Lin, Q.-h.; Li, S.-s.; Jia, Z.; Han, T.; Lin, R.-c.; Wang, D.; Wei, F.; Li, X.-r., Identification of mountain-cultivated ginseng and cultivated ginseng using UPLC/oa-TOF MSE with a multivariate statistical sample-profiling strategy. J. Ginseng Res. 2016, 40, 344-350. Xu, X.-F.; Xu, S.-Y.; Zhang, Y.; Zhang, H.; Liu, M.-N.; Liu, H.; Gao, Y.; Xue, X.; Xiong, H.; Lin, R.-C.; Li, X.-R., Chemical Comparison of Two Drying Methods of Mountain Cultivated Ginseng by UPLC-QTOF-MS/MS and Multivariate Statistical Analysis. Molecules 2017, 22, 717. Xu, X.-F.; Gao, Y.; Xu, S.-Y.; Liu, H.; Xue, X.; Zhang, Y.; Zhang, H.; Liu, M.-N.; Xiong, H.; Lin, R.-C.; Li, X.-R., Remarkable impact of steam temperature on ginsenosides transformation from fresh ginseng to red ginseng. J. Ginseng Res. 2018, 42, 277-287. Yang, W.-z.; Ye, M.; Qiao, X.; Liu, C.-f.; Miao, W.-j.; Bo, T.; Tao, H.-y.; Guo, D.-a., A strategy for efficient discovery of new natural compounds by integrating orthogonal column chromatography and liquid chromatography/mass spectrometry analysis: Its application in Panax ginseng, Panax quinquefolium and Panax notoginseng to characterize 437 potential new ginsenosides. Anal. Chim. Acta 2012, 739, 56-66. Yang, W.-Z.; Bo, T.; Ji, S.; Qiao, X.; Guo, D.-A.; Ye, M., Rapid chemical profiling of saponins in the flower buds of Panax notoginseng by integrating MCI gel column chromatography and liquid chromatography/mass spectrometry analysis. Food Chem. 2013, 139, 762-769. Yang, W.-z.; Hu, Y.; Wu, W.-y.; Ye, M.; Guo, D.-a., Saponins in the genus Panax L. (Araliaceae): A systematic review of their chemical diversity. Phytochemistry 2014, 106, 7-24. Yang, W.; Qiao, X.; Li, K.; Fan, J.; Bo, T.; Guo, D.-a.; Ye, M., Identification and differentiation of Panax ginseng, Panax quinquefolium, and Panax notoginseng by monitoring multiple diagnostic chemical markers. Acta Pharm. Sin. B 2016, 6, 568-575. Yang, Y.; Liang, X.; Jin, P.; Li, N.; Zhang, Q.; Yan, W.; Zhang, H.; Sun, J., Screening and determination for potential acetylcholinesterase inhibitory constituents from ginseng stem–leaf saponins using ultrafiltration (UF)-LC-ESI-MS2. Phytochem. Anal. 2019, 30, 26-33. Yang, Y.; Ju, Z.; Yang, Y.; Zhang, Y.; Yang, L.; Wang, Z., Phytochemical analysis of Panax species: a review. J. Ginseng Res. 2020. Yu, S. E.; Mwesige, B.; Yi, Y.-S.; Yoo, B. C., Ginsenosides: the need to move forward from bench to clinical trials. J. Ginseng Res. 2018. Zhang, C.; Guan, F.; Zhang, L., A preliminary study on the improvement of protopanaxadiol yield by alkaline hydrolysis. Journal of Baotou Medical College 2011, 27, 1-5. Zhang, C.; Zuo, T.; Wang, X.; Wang, H.; Hu, Y.; Li, Z.; Li, W.; Jia, L.; Qian, Y.; Yang, W., Integration of Data-Dependent Acquisition (DDA) and Data-Independent High-Definition MSE (HDMSE) for the Comprehensive Profiling and Characterization of Multicomponents from Panax japonicus by UHPLC/IM-QTOF-MS. Molecules 2019, 24, 2708. Zhang, H.-M.; Li, S.-L.; Zhang, H.; Wang, Y.; Zhao, Z.-L.; Chen, S.-L.; Xu, H.-X., Holistic quality evaluation of commercial white and red ginseng using a UPLC-QTOF-MS/MS-based metabolomics approach. J. Pharm. Biomed. Anal. 2012, 62, 258-273. Zhang, X.; Song, F.; Cui, M.; Liu, Z.; Liu, S., Investigation of the hydrolysis of ginsenosides by high performance liquid chromatography-electrospray ionization mass spectrometry. Planta Med. 2007, 73, 1225-1229. Zheng, M.-m.; Xu, F.-x.; Li, Y.-j.; Xi, X.-z.; Cui, X.-w.; Han, C.-c.; Zhang, X.-l., Study on Transformation of Ginsenosides in Different Methods. Biomed Res. Int. 2017, 2017, 10. Zhou, S.-S.; Xu, J.-D.; Zhu, H.; Shen, H.; Xu, J.; Mao, Q.; Li, S.-L.; Yan, R., Simultaneous determination of original, degraded ginsenosides and aglycones by ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry for quantitative evaluation of Du-Shen-Tang, the decoction of ginseng. Molecules 2014, 19, 4083-4104. Zhu, H.; Lin, H.; Tan, J.; Wang, C.; Wang, H.; Wu, F.; Dong, Q.; Liu, Y.; Li, P.; Liu, J., UPLC-QTOF/MS-based nontargeted metabolomic analysis of mountain-and garden-cultivated Ginseng of different ages in Northeast China. Molecules 2019, 24, 33. Zuo, T.; Zhang, C.; Li, W.; Wang, H.; Hu, Y.; Yang, W.; Jia, L.; Wang, X.; Gao, X.; Guo, D., Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling four-dimensional separation and characterization of the multicomponents from white ginseng and red ginseng. J. Pharm. Anal. 2019. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72982 | - |
dc.description.abstract | 人參皂苷 (ginsenosides) 為人參 (ginseng) 中主要生理活性成分。為了因應人參原料及其萃取物品質管制之需求,本研究使用液相層析串聯高解析度軌道阱質譜儀 (ultra-high performance liquid chromatography coupled to high resolution (Orbitrap) mass spectrometry, LC-HRMS/MS) 建立人參皂苷及其相關化合物化學指紋圖 (chemical fingerprint profile) 之分析平台。此分析平台可辨識出62種人參皂苷及4種皂苷元 (sapogenins) ,其中包含17種原人參三醇 (protopanaxatriol, PPT)、34種原人參二醇 (protopanaxadiol, PPD) 、4種奧克梯隆型 (ocotillol type, OT)、4種齊墩果烷酸型 (oleanolic acid type, OA) 及7種C-17分支多變型 (C17 side-chain varied type) 人參皂苷。分析平台亦可辨識6種乙醯化 (acetyl-) 、8種丙二醯化 (malonyl-)、1種丁醯化 (butenoyl-) 及1種辛醯化 (octenoyl-) 人參皂苷。研究結果顯示亞洲參根、西洋參根及三七根皆未含有皂苷元(苷元);各品種人參之人參皂苷以PPT型及PPD型人參皂苷為大宗,而OT型、OA型人參皂苷和C-17分支多變型人參皂苷則是次要成分。針對可取得的樣品發現西洋參之次要人參皂苷成分相較於亞洲參和三七來得豐富。本研究亦發現紅參中低極性人參皂苷含量落在0.0875 至0.2708 mg/g範圍顯著高於新鮮人參,顯示出加工製備所產之紅參在熱處理下的變化。根據人參皂苷之組成,藉由主成分分析 (principal components analysis) 區分亞洲參、西洋參及三七。本研究利用此分析平台可有效辨識人參皂苷及皂苷元指紋圖譜及應用於人參原料和相關產品進行品質管制及加工監控指標之用途。 | zh_TW |
dc.description.abstract | Ginsenosides are the major bioactive ingredients in ginseng. By responding to the demanding for quality control of the raw materials and extracts of ginseng, this study established a platform for chemical fingerprint profile of ginsenosides and their related compounds on an ultra-high performance liquid chromatography coupled to high resolution (Orbitrap) mass spectrometry (LC-HRMS/MS). The platform can detect 62 ginsenosides and 4 sapogenins, including 17 protopanaxatriol-type (PPT), 34 protopanaxadiol-type (PPD), 4 ocotillol-type (OT), 4 oleanolic acid-type (OA) and 7 C17 side chain varied-type ginsenosides. There were also 6 acetyl-ginsenosides, 8 malonyl-ginsenosides, 1 butenoyl- ginsenosides, 1 octenoyl-ginsenosides that can be identified. We did not find any sapogenin, aglycone, in selected raw materials, including radix of Panax ginseng, Panax quinquefolius, and Panax notoginseng. The PPT- and PPD-type components were major ginsenosides in Panax species with less content of OT-type, OA-type, and C17 side chain varied-type ginsenosides. For comparison of those minor constituents in the selected samples, P. quinquefolius were higher than those in P. ginseng and P. notoginseng. The content of low polarity ginsenosides in red ginsengs was in the range of 0.0875 to 0.2708 mg/g, which were significantly higher than fresh ginsengs. It could be the result of the thermal treatment of the red ginseng preparing process. According to their ginsenoside composition, the statistical principal component analysis can differentiate P. quinquefolius, P. ginseng, and P. notoginseng. The analytical platform established can be an effective tool to obtain the fingerprint of ginsenosides and sapogenins for quality control of ginseng raw material and products and indices of process monitoring. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T07:12:41Z (GMT). No. of bitstreams: 1 U0001-2912202010503100.pdf: 24414958 bytes, checksum: 0e9a642ca81df355202d2e2fd44e9c21 (MD5) Previous issue date: 2021 | en |
dc.description.tableofcontents | 誌謝 I 摘要 II Abstract III 目錄 IV 圖目錄 VI 表目錄 XVI 壹、前言 1 貳、文獻回顧 2 第一章、人參 2 1.1人參簡介 2 1.2人參生理活性成分 3 第二章、人參皂苷 3 2.1人參皂苷結構 3 2.2人參皂苷生合成 6 2.3人參皂苷分布情形及人參品種和生理活性相關性 9 2.4人參皂苷結構與抑制癌細胞生長之關係 10 2.5人參皂苷在食品加工下結構轉變 11 第三章、人參皂苷結構分析 14 3.1萃取人參皂苷方法 14 3.2高效能液相層析 15 3.3質譜結構分析 16 3.4核磁共振 19 第四章、藥用植物之指紋圖譜 20 4.1化學標記及分析方法 20 4.2人參皂苷應用於人參品種鑑定 21 4.3以人參皂苷作為人參產品品質管制指標 22 參、研究目的及架構 23 肆、材料與方法 24 第一章、實驗材料 24 1.1人參樣品列表 24 1.2空白基質 24 第二章、實驗藥品 25 2.1標準品 25 2.2化學藥品 25 第三章、實驗儀器及數據處理軟體 26 3.1前處理儀器設備 26 3.2超高效液相層析串聯質譜儀 26 3.3數據處理軟體 27 第四章、實驗方法 27 4.1樣品前處理 27 4.2酸水解法 27 4.3層析儀移動相配置 28 4.4層析儀相關參數設定 28 4.5質譜儀參數設定 29 4.6人參皂苷及皂苷元定量分析 30 伍、結果與討論 32 第一章、人參樣品前處理 32 第二章、人參皂苷以超高效能液相層析串聯高解析質譜法分析 32 2.1人參皂苷以超高效能液相層析儀分離 32 2.2人參皂苷以高解析質譜儀偵測 42 2.3人參皂苷分析平台之檢量線、定量極限及回收率 62 第三章、人參中皂苷元及人參皂苷分布情形 64 3.1人參中皂苷元分布情形 64 3.2人參中人參皂苷分布情形 64 3.3人參中人參皂苷主成分分析 90 陸、結論 93 柒、參考文獻 94 捌、附錄 103 第一章、品管樣品中人參皂苷及皂苷元滯留時間和濃度之品質管制 103 第二章、高解析質譜鑑定人參皂苷及皂苷元 103 | |
dc.language.iso | zh-TW | |
dc.title | 利用液相層析串聯高解析質譜法建立人參皂苷及皂苷元分析平台 | zh_TW |
dc.title | Establishment of analytical platform for ginsenosides and sapogenins by UHPLC-MS/MS | en |
dc.type | Thesis | |
dc.date.schoolyear | 109-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張永和(Yung-Ho Chang),陳頌方(Sung-Fang Chen),羅翊禎(Yi-Chen Lo),邱群惠(Chun-Hui Chiu) | |
dc.subject.keyword | 人參,人參皂苷,液相層析串聯高解析度質譜法,化學指紋, | zh_TW |
dc.subject.keyword | Ginseng,Ginsenoside,LC-HRMS/MS,Chemical fingerprint, | en |
dc.relation.page | 233 | |
dc.identifier.doi | 10.6342/NTU202004474 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2021-01-19 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
U0001-2912202010503100.pdf 目前未授權公開取用 | 23.84 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。