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Title: | 以3D列印方式利用投影機製造胎盤蛻膜間質幹細胞之細胞片 Projector-based 3D printing for pcMSC cell sheets |
Authors: | Hung-Hao Chang 張紘豪 |
Advisor: | 郭柏齡(Po-Ling Kuo) |
Keyword: | 3D列印,胎盤蛻膜間質幹細胞,甲基丙烯酸酐化明膠,苯基(2,4,6-三甲基苯甲?基)磷酸鋰鹽,細胞片, 3D printing,human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs),Gelatin Methacryloyl(GelMa),Lithium Phenyl(2,4,6-trimethylbenzoyl)phosphinate (LAP),pcMSC cell sheets, |
Publication Year : | 2019 |
Degree: | 碩士 |
Abstract: | 此篇論文描述以3D列印原理,使用投影機完成幹細胞之細胞片,這個方式包含了3D列印機、印列墨水的製造,以及膠上幹細胞的培養。3D列印機選擇用市售的投影機,經過改裝,利用其投影系統投射,使印列墨水曝光而凝固成膠,並且可依投影圖形,製造出不同的形狀膠體。印列墨水主要成分為:甲基丙烯酸酐化明膠(GelMa)和苯基(2,4,6-三甲基苯甲酰基)磷酸鋰鹽。細胞培養選擇用胎盤蛻膜間質幹細胞(pcMSCs),其可以附著並培養於膠上。在往後的研究,因為3D列印的特性,可以完成孔洞狀結構,讓培養液可以更快流通於膠內。而以此方式所完成之胎盤蛻膜間質幹細胞之細胞片,可用於臨床研究,治療皮膚嚴重燒燙傷之傷口復原。 In this article described a method which basing on 3D printing to producing stem cell sheets by using projector. This method included the 3D printing machine, the manufacture for printing gel and the stem cell culture. The printing gel is exposed and solidified by light projector system. The light projector system was remodeled from the commercial projector. Moreover, the outlook of the gel can be designed by the exposed pattern from projector. The printing gel is comprised by Gelatin Methacryloyl(GelMa), Lithium Phenyl(2,4,6-trimethylbenzoyl)phosphinate (LAP). In the cell culture, I choose the human placenta chorionic decidual-derived MSCs(pcMSCs). It can adhere and culture on the gel. In future work, because of the features of 3D printing, the gel can be designed with porous structure. This structure can allow the medium flowing faster in the gel. The final product, pcMSC cell sheets, completed by this method, can be used in clinical research and severe burn wound treatment. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7210 |
DOI: | 10.6342/NTU201902957 |
Fulltext Rights: | 同意授權(全球公開) |
Appears in Collections: | 生醫電子與資訊學研究所 |
Files in This Item:
File | Size | Format | |
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ntu-108-1.pdf | 1.94 MB | Adobe PDF | View/Open |
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