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標題: | 核仁蛋白 SAHY9/APUM23透過離層酸訊息傳遞影響阿拉伯芥鹽逆境反應之研究 SALT HYPERSENSITIVE MUTANT 9, a nucleolar APUM23 protein, is essential for salt sensitivity in association with the ABA signaling pathway in Arabidopsis |
作者: | Kai-Chau Huang 黃楷超 |
指導教授: | 鄭萬興(Wan-Hsing Cheng) |
關鍵字: | 離層酸,SAHY9,APUM23,核仁,轉錄體,蛋白質體,鹽類逆境, ABA,APUM23,nucleolus,proteome,salt stress,transcriptome, |
出版年 : | 2018 |
學位: | 博士 |
摘要: | 近年來大規模的乾旱與人為的過度施肥,造成耕作地區的土壤嚴重鹽化。植物生長於高度鹽化的土壤會降低其產量與生長速度,造成糧食作物的短缺。為研究植物如何克服鹽逆境的機制,本研究利用遺傳學方法發現阿拉伯芥鹽逆境敏感突變株(sahy9),分析發現該基因為影響核醣體RNA合成與葉片發育的核仁基因APUM23,但此基因在鹽類逆境中扮演的功能尚未知。分析生理性狀,sahy9 / apum23突變株發芽後幼苗發育緩慢,在各種鹽類逆境的長期處理後,與正常植株相比有較高的死亡率。sahy9 / apum23突變株在鹽類處理後的轉錄體和蛋白質體表現,發現其主要影響細胞和代謝過程、非生物和生物逆境反應。然而轉錄體和蛋白質體的基因表現與正常株的重疊率非常低(約12%),這證明了轉錄後修飾的機制參與了SAHY9/APUM23調控植物的對鹽逆境的適應。這些差異性表現的基因和蛋白質,主要包含了離層酸的合成和信息傳遞,非生物性逆境和核醣體生合成相關基因。其中NCED3,ABI2,PP2CA和ABA反應基因(如RD20和RD29B)的基因與蛋白質表現在sahy9 / apum23突變株內顯著的受到抑制,同時離層酸的合成量也較正常植株少;此外,有一群LEA (Late embryo abundant) 基因/蛋白質的表現也受到影響。sahy9 / apum23突變株在鹽逆境處理中的鹽敏感性狀與離層酸合成量可利用外加離層酸恢復。整體來說, SAHY9 / APUM23可藉由離層酸訊息傳遞和其下游反應基因影響阿拉伯芥的鹽類逆境反應。 To date, soil salinity is getting worse in the arable land because of drought stress and over application of fertilizer. High salinity is a major environmental factor, which hinders plant growth and productivity. To better understand the regulatory mechanisms by which plants cope with salt stress, we took genetic approaches to identify salt hypersensitive mutant 9 (sahy9), a new allele of apum23, in Arabidopsis thaliana. The seedlings of sahy9/apum23 mutant displayed postgermination developmental arrest and then became bleached after prolonged culture under various salt stressors. Transcriptomic and proteomic analyses of salt-treated sahy9/apum23 and wild-type seedlings revealed differential expression of genes/proteins that have similar functional categories of biological processes, primarily those involved in cellular and metabolic processes as well as abiotic and biotic stress responses. However, the consistency of differential gene expression at both the transcript and protein levels was low (~12%), which suggests the involvement of posttranscriptional processing during the salt response. Furthermore, the altered expression of genes and proteins mediated by SAHY9/APUM23 regarding salt sensitivity involves abscisic acid (ABA) biosynthesis and signaling, abiotic stress responses, and ribosome biogenesis-related genes. Importantly, NCED3, ABI2, PP2CA, and major ABA-responsive marker genes, such as RD20 and RD29B, were down-regulated at both the transcript and protein levels in conjunction with lower contents of ABA and changes in the expression of a subset of LEA proteins in sahy9/apum23 mutants under salt stress. Moreover, the salt hypersensitivity of sahy9/apum23 mutant was largely rescued by the exogenous application of ABA during salt stress. Collectively, these data suggest that the SAHY9/APUM23-mediated salt response is associated with the ABA signaling pathway and its downstream stress responsive network. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69807 |
DOI: | 10.6342/NTU201800659 |
全文授權: | 有償授權 |
顯示於系所單位: | 植物科學研究所 |
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