請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69807完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭萬興(Wan-Hsing Cheng) | |
| dc.contributor.author | Kai-Chau Huang | en |
| dc.contributor.author | 黃楷超 | zh_TW |
| dc.date.accessioned | 2021-06-17T03:28:48Z | - |
| dc.date.available | 2018-04-18 | |
| dc.date.copyright | 2018-04-18 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-02-27 | |
| dc.identifier.citation | Abbasi N, Kim HB, Park NI, Kim YK, Park YI, Choi SB. APUM23, a nucleolar PUF domain RNA-binding protein, is required for pre-ribosomal RNA processing and normal growth patterning in Arabidopsis. Plant J. 2010;64:960-76.
Abbasi N, Park YI, Choi SB. Pumilio Puf domain RNA-binding proteins in Arabidopsis. Plant Signal Behav. 2011;6:364-68. Alba MM, Pages M. Plant proteins containing the RNA-recognition motif. Trends Plant Sci. 1998;3:15-21. Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, et al. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003;301:653-57. Barakat A, Szick-Miranda K, Chang IF, Guyot R, Blanc G, Cooke R, et al. The organization of cytoplasmic ribosomal protein genes in the Arabidopsis genome. Plant Physiol. 2001;127:398-415. Bartels D, Sunkar R. Drought and salt tolerance in plants. CRC Crit Rev Plant Sci. 2005;24:23-58. Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil. 1973;39:205-07. Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. JR Statist Soc B. 1995;57:289-300. Boisvert FM, van Koningsbruggen S, Navascués J, Lamond AI. The multifunctional nucleolus. Nat Rev Mol Cell Biol. 2007;8:574-85. Boulon S, Westman BJ, Hutten S, Boisvert FM, Lamond AI. The nucleolus under stress. Mol Cell. 2010;40:216-27. Candat A, Paszkiewicz G, Neveu M, Gautier R, Logan DC, Avelange-Macherel M, et al. The ubiquitous distribution of late embryogenesis abundant proteins across cell compartments in Arabidopsis offers tailored protection against abiotic stress. Plant Cell. 2014;26:3148-66. Carroll AJ. The Arabidopsis cytosolic ribosomal proteome: from form to function. Front Plant Sci. 2013;4:1-14. Chen YS, Lo SF, Sun PK, Lu CA, Ho TH, Yu SM. A late embryogenesis abundant protein HAV1 regulated by an inducible promoter enhances root growth and abiotic stress tolerance in rice without yield penalty. Plant Biotech J. 2015;13:105-16. Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR. Abscisic acid: emergence of a core signaling network. Annu Rev Plant Biol. 2010;61:651-79. Dalal M, Trayal D, Chinnusamy V, and Bansal KC. Abiotic stress and ABA-inducible group 4 LEA from Brassica napus plays a key role in salt and drought tolerance. J Biotech. 2009;139:137-145. Degenhardt RF, Bonham-Smith PC. Arabidopsis ribosomal proteins RPL23aA and RPL23aB are differentially targeted to the nucleolus and are disparately required for normal development. Plant Physiol. 2008;147:128-42. Delvillani F, Papiani G, Dehò G, Briani F. S1 ribosomal protein and the interplay between translation and mRNA decay. Nucleic Acids Res. 2011;39:7702–15. Finkelstein RR, Reeves W, Ariizumi T, Steber C. Molecular aspects of seed dormancy. Annu Rev Plant Biol. 2008;59:387-415. Floris M, Mahgoub H, Lanet E, Robaglia C, Menand B. Post-transcriptional regulation of gene expression in plants during abiotic stress. Int J Mol Sci. 2009;10:3168-85. Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968;50:151-8. Goldstrohm AC, Seay DJ, Hook BA, Wickens M. PUF protein-mediated deadenylation is catalyzed by Ccr4p. J Biol Chem. 2007;282:109–14. Hammani K, Giege P. RNA metabolism in plant mitochondria. Trends Plant Sci. 2014;19:380–9. Heath RL, Packer L. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968;125:189-98. Hoth S, Morgante M, Sanchez JP, Hanafey MK, Tingey SV, Chua NH. Genome-wide gene expression profiling in Arabidopsis thaliana reveals new targets of abscisic acid and largely impaired gene regulation in the abi1-1 mutant. J. Cell Sci. 2002;115:4891-900. Hundertmark M, Hincha DK. LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana. BMC Genomic. 2008;9:118-40. Hwang SG, Chen HC, Huang WY, Chu YC, Shii CT, Cheng, WH. Ectopic expression of rice OsNCED3 in Arabidopsis increases ABA level and alters leaf morphology. Plant Sci. 2010;178:12-22. Ingram J, Bartels D. The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol. 1996;47:377-403. Kim TH, Bohmer M, Hu H, Nishimura N, Schroeder JI. Guard cell signal transduction network: advances in understanding abscisic acid, CO2, and Ca2+ signaling. Annu. Rev. Plant Biol. 2010;61:561-91. Lan P, Li W, Wen TN, Shiau JY, Wu YC, Lin W, Schmidt W. iTRAQ protein profile analysis of Arabidopsis roots reveals new aspects critical for iron homeostasis. Plant Physiol. 2011;155:821-34. Lefebvre V, North H, Frey A, Sotta B, Seo M, Okamoto M, Nambara E, and Marion-Poll A. Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy. Plant J. 2006;45:309-19. Liu MJ, Wu SH, Chen HM, Wu SH. Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis. Mol Sys Biol. 2012;8:566. Lorkovic ZJ, Barta A. Genome analysis: RNA recognition motif (RRM) and K homology (KH) domain RNA-binding proteins from the flowering plant Arabidopsis thaliana. Nucleic Acids Res. 2002;30:623-35. Luan S. The CBL-CIPK network in plant calcium signaling. Trends Plant Sci. 2009;14:37-42. Ma Y, Szostkiewicz I, Korte A, Moes D, Yang Y, Christmann A, et al. Regulators of PP2C phosphatase activity function as abscisic acid sensors. Science. 2009;324:1064-8. Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R. Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ. 2010;33:453-67. Munns R. Genes and salt tolerance bringing them together. New Phytol. 2005;167:645-63. Munns R, Tester M. Mechanisms of salinity tolerance. Ann Rev Plant Biol. 2008;59:651-81. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 1962;15:473-97. Pardo JM. Biotechnology of water and salinity stress tolerance. Curr Opin Biotech. 2010;21:339-46. Park SY, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, et al. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science. 2009;324:1068-71. Petrica JJ, Nelson TM. Arabidopsis nucleolin affects plant development and patterning. Plant Physiol. 2007;144:173-86. Pontvianne F, Matía I, Douet J, Tourmente S, Medina FJ, Echeverria M, et al. Characterization of AtNUC-L1 reveals a central role of nucleolin in nucleolus organization and silencing of AtNUC-L2 gene in Arabidopsis. Mol Biol Cell. 2007;18:369-79. Rosado A, Sohn EJ, Drakakaki G, Pan S, Swidergal A, Xiong Y, et al. Auxin-mediated ribosomal biogenesis regulates vacuolar trafficking in Arabidopsis. Plant Cell. 2010;22:143-58. Saha B, Mishra S, Awasthi JP, Sahoo L, Panda S.K. Enhanced drought and salinity tolerance in transgenic mustard [Brassica juncea (L.) Czern & Coss.] overexpressing Arabidopsis group 4 late embryogenesis abundant gene (AtLEA4-1). Environ Exp Bot. 2016;128: 99-111. Seckin B, Turkan I, Sekmen AH, Ozfidan C. The role of antioxidant defense systems at differential salt tolerance of Hordeum marinum Huds. (sea barleygrass) and Hordeum vulgare L. (cultivated barley). Environ Exp Bot. 2010;69:76-85. Shalata A, Tal M. The effect of salt stress on lipid peroxidation and antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Physiol. Plant. 1998;104:169-74. Shaw P, Brown J. Nucleoli: composition, function, and dynamics. Plant Physiol. 2012;158: 44-51. Simpson GG, Filipowicz W. Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organization of the spliceosomal machinery. Plant Mol Biol. 1996;32:1-41. Spassov DS, Jurecic R. The PUF family of RNA binding proteins: does evolutionarily conserved structure equal conserved function? IUBMB Life. 2003;55:359–66. Sripinyowanich S, Chamnanmanoontham N, Udomchalothorn T, Maneeprasopsuk S, Santawee P, Buaboocha T, et al. Overexpression of a partial fragment of the salt-responsive gene OsNUC1 enhances salt adaptation in transgenic Arabidopsis thaliana and rice (Oryza sativa L.) during salt stress. Plant Sci. 2013;213:67-78. Stern DB, Goldschmidt-Clermont M, Hanson MR. Chloroplast RNA metabolism. Annu Rev Plant Biol. 2010;61:125–55. Tian T, Liu Y, Yan H, You Q, Yi X, Du Z, et al. agriGO v2.0: a GO analysis toolkit for the agricultural community, 2017 update. Nucleic acid Res. 2017;45:W122-W129. Tong CC, Reichler S, Blumenthal S, Balk J, Hsieh HL, Roux SJ. Light regulation of the abundance of mRNA encoding a nucleolin-like protein localized in the nucleoli of pea nuclei. Plant Physiol. 1997;114:643–52. Udomchalothorn T, Plaimas K, Sripinyowanich S, Boonchai C, Kojonna T, Chutimanukul P, et al. OsNucleolin1-L expression in Arabidopsis enhances photosynthesis via transcriptome modification under salt stress conditions. Plant Cell Physiol. 2017;58:717-34. Verslues PE, Sharma S. Proline metabolism and its implications for plant-environment interaction. The Arabidopsis Book. 2010; e0140. Weinl S, Kudla J. The CBL-CIPK Ca2+-decoding signaling network: function and perspectives. New Phytol. 2009;184:517-28. Weis BL, Kovacevic J, Missbach S, Schleiff E. Plant-specific features of ribosome biogenesis. Trends Plant Sci. 2015;20:729-40. Welin BV, Olson Å, Nylander M, Palva ET. Characterization and differential expression of dhn/lea/rab-like genes during cold acclimation and drought stress in Arabidopsis thaliana. Plant Mol Biol. 1994;26: 131-44. Xu D, Duan X, Wang B, Hong B, Ho THD, Wu R. Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol. 1996;110:249-57. Yoshida T, Mogami J, Yamaguchi-Shinozaki K. Omics approaches toward defining the comprehensive abscisic acid signaling network in plants. Plant Cell Physiol. 2015;56:1043-52. Zhao H, Lu S, Li R, Chen T, Zhang H, Cui P, et al. The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes. J Exp Bot. 2015;66:6863-75. Zhu J, Fu X, Koo YD, Zhu JK, Jenny FE Jr, Adams MWW, et al. An enhancer mutant of Arabidopsis salt overly sensitive 3 mediates both ion homeostasis and the oxidative stress response. Mol Cell Biol. 2007;27:5214-24. Zhu JK. Salt and drought signal transduction in plants. Annu Rev Plant Biol. 2002;53:247-73. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69807 | - |
| dc.description.abstract | 近年來大規模的乾旱與人為的過度施肥，造成耕作地區的土壤嚴重鹽化。植物生長於高度鹽化的土壤會降低其產量與生長速度，造成糧食作物的短缺。為研究植物如何克服鹽逆境的機制，本研究利用遺傳學方法發現阿拉伯芥鹽逆境敏感突變株(sahy9)，分析發現該基因為影響核醣體RNA合成與葉片發育的核仁基因APUM23，但此基因在鹽類逆境中扮演的功能尚未知。分析生理性狀，sahy9 / apum23突變株發芽後幼苗發育緩慢，在各種鹽類逆境的長期處理後，與正常植株相比有較高的死亡率。sahy9 / apum23突變株在鹽類處理後的轉錄體和蛋白質體表現，發現其主要影響細胞和代謝過程、非生物和生物逆境反應。然而轉錄體和蛋白質體的基因表現與正常株的重疊率非常低（約12％），這證明了轉錄後修飾的機制參與了SAHY9/APUM23調控植物的對鹽逆境的適應。這些差異性表現的基因和蛋白質，主要包含了離層酸的合成和信息傳遞，非生物性逆境和核醣體生合成相關基因。其中NCED3，ABI2，PP2CA和ABA反應基因（如RD20和RD29B）的基因與蛋白質表現在sahy9 / apum23突變株內顯著的受到抑制，同時離層酸的合成量也較正常植株少；此外，有一群LEA (Late embryo abundant) 基因/蛋白質的表現也受到影響。sahy9 / apum23突變株在鹽逆境處理中的鹽敏感性狀與離層酸合成量可利用外加離層酸恢復。整體來說， SAHY9 / APUM23可藉由離層酸訊息傳遞和其下游反應基因影響阿拉伯芥的鹽類逆境反應。 | zh_TW |
| dc.description.abstract | To date, soil salinity is getting worse in the arable land because of drought stress and over application of fertilizer. High salinity is a major environmental factor, which hinders plant growth and productivity. To better understand the regulatory mechanisms by which plants cope with salt stress, we took genetic approaches to identify salt hypersensitive mutant 9 (sahy9), a new allele of apum23, in Arabidopsis thaliana. The seedlings of sahy9/apum23 mutant displayed postgermination developmental arrest and then became bleached after prolonged culture under various salt stressors. Transcriptomic and proteomic analyses of salt-treated sahy9/apum23 and wild-type seedlings revealed differential expression of genes/proteins that have similar functional categories of biological processes, primarily those involved in cellular and metabolic processes as well as abiotic and biotic stress responses. However, the consistency of differential gene expression at both the transcript and protein levels was low (~12%), which suggests the involvement of posttranscriptional processing during the salt response. Furthermore, the altered expression of genes and proteins mediated by SAHY9/APUM23 regarding salt sensitivity involves abscisic acid (ABA) biosynthesis and signaling, abiotic stress responses, and ribosome biogenesis-related genes. Importantly, NCED3, ABI2, PP2CA, and major ABA-responsive marker genes, such as RD20 and RD29B, were down-regulated at both the transcript and protein levels in conjunction with lower contents of ABA and changes in the expression of a subset of LEA proteins in sahy9/apum23 mutants under salt stress. Moreover, the salt hypersensitivity of sahy9/apum23 mutant was largely rescued by the exogenous application of ABA during salt stress. Collectively, these data suggest that the SAHY9/APUM23-mediated salt response is associated with the ABA signaling pathway and its downstream stress responsive network. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T03:28:48Z (GMT). No. of bitstreams: 1 ntu-107-D99b42009-1.pdf: 5613146 bytes, checksum: 40b0620201d0c3bd6ad1ef1a445df8c3 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 目錄
致謝……………………………………………………………………………….……i 摘要……………………………………………………………………………………ii Abstract………………………...………………….………………………………….iii 1. Introduction…………………………………………..……………………………..1 2. Materials and Methods………………………………………………………………5 2.1 Plant materials and growth conditions……………………………….………5 2.2 Genetic isolation of sahy9……………..……………………………………..5 2.3 Various salt treatments……………………………..…………………………6 2.4 MDA assay………………………………...…………………………………6 2.5 Microarray analysis………………………………………….……………….7 2.6 Total protein extraction iTRAQ analysis…………………………………….8 2.7 Quantitative RT-PCR……………………………………...…………………8 2.8 Ribosome profile analyses…………………………………………..……….9 2.9 ABA and proline assays……………...……………………………………..10 2.10 Measurement of germination rate……………………….………………..10 3. Results………………………………………………...………………………….11 3.1 salt hypersensitive mutant 9 (sahy9) is a new allele of apum23………...……11 3.2 Mutation of SAHY9/APUM23 alters sensitivity to various salt treatments………………………………………………………….……………12 3.3 Transcriptomic analysis reveals differential expression of genes involved in ABA and stress responses in sahy9/apum23 mutants under salt stress……….......13 3.4 Mutation of SAHY9/APUM23 alters the expression of genes involved in ribosome biogenesis and ribosome abundance under high salinity conditions.…..16 3.5 Changes in the protein expression profile of the sahy9/apum23 mutant……..17 3.6 Differential expression of proteins involved in ribosome biogenesis in sahy9/apum23 mutants under normal and salt stress conditions……….………...19 3.7 Altered expression of proteins involved in ABA and stress responses in the sahy9/apum23 mutants…………………………………….………………….…20 3.8 Exogenous application of ABA largely rescues the salt hypersensitivity of sahy9/apum23 seedlings under salt stress conditions……………….…………....22 4. Discussion………………………………………………………………………….24 4.1 Changes in the expression of ribosome biogenesis-related genes and ribosome abundance in sahy9/apum23 under normal and salt stress conditions……...…...24 4.2 Genome-wide analyses of gene expression reveal similar functional categories but low consistency of transcript and protein profiles in sahy9/apum23 mutants under salt stress conditions……….………………………………………….…27 4.3 SAHY9/APUM23 regulates salt sensitivity in association with the ABA signaling pathway and ABA-mediated downstream stress-responsive or tolerance genes………………………………………..………………………………….28 5. Conclusion………………………………………………………………….…….33 6. Acknowledgements……………………………………………………………..…34 7. References…………...……………………………………………………..…….35 圖目錄 Figure. 1 The sahy9/apum23 mutants show salt hypersensitivity………………….…44 Figure. 2 The sahy9/apum23 mutants are sensitive to various salt stresses…………..45 Figure. 3 Functional categorization of differentially expressed genes by annotation of GO biological processes…………………………………..……………….46 Figure. 4 Validation of genes involved in ABA and salt stress responses…………….47 Figure. 5 Changes in the ribosome subunit profile in the sahy9/apum23 mutant……4/ Figure. 6 Overlap of identified transcripts and proteins in sahy9/apum23 under normal or salt stress conditions……………………………..…..……………….....49 Figure. 7 Functional categorization of differentially expressed proteins in sahy9/apum23 compared with the wild type………………....…………….50 Figure. 8 ABA contents and exogenous application of ABA in the sahy9/apum23 mutant and wild type…………………………...………………………….51 Figure S1. SAHY9/APUM23 gene structure showing the T-DNA insertion sites.....…52 Figure S2. Effect of osmotic stress on sahy9/apum23 mutant plants………………....53 Figure S3. MDA contents………………….…………………………………………54 Figure S4. qRT-PCR results of stress-responsive genes and proline contents………...55 Figure S5. Validation of the expression of genes involved in ribosome biogenesis in sahy9/apum23 under salt stress conditions………………..……..………...56 Figure S6. Expression of genes regulated by ABA and salt stress……………………57 Figure S7. ABA contents……………………………………………………………..58 Figure S8. Germination rate analysis………………………………………………...59 表目錄 Table 1. Differential expression of genes involved in ABA and abiotic stress responses in sahy9/apum23 under salt stress…………………………………..……60 Table 2. Differential expression of genes involved in ribosome biogenesis in sahy9/apum23 under salt stress…………………………………………...…62 Table 3. Differential expression of proteins involved in ribosome biogenesis in sahy9/apum23 under normal and salt stress conditions………….……….….63 Table 4. Differential expression of proteins involved in ABA and abiotic stress responses in sahy9/apum23 under normal and salt stress conditions.……….66 Table 5. Differential expression of LEA proteins in the sahy9/apum23 mutant compared with the wild type………………………………...………………69 Table S1. Overlap of differential gene expression at both the transcript and protein levels in the sahy9/apum23 mutants under salt stress conditions……...…….70 Table S2. Primer sequences used in this study………………………………………75 | |
| dc.language.iso | en | |
| dc.subject | 核仁 | zh_TW |
| dc.subject | 蛋白質體 | zh_TW |
| dc.subject | 鹽類逆境 | zh_TW |
| dc.subject | 離層酸 | zh_TW |
| dc.subject | SAHY9 | zh_TW |
| dc.subject | APUM23 | zh_TW |
| dc.subject | 轉錄體 | zh_TW |
| dc.subject | salt stress | en |
| dc.subject | ABA | en |
| dc.subject | APUM23 | en |
| dc.subject | nucleolus | en |
| dc.subject | proteome | en |
| dc.subject | transcriptome | en |
| dc.title | 核仁蛋白 SAHY9/APUM23透過離層酸訊息傳遞影響阿拉伯芥鹽逆境反應之研究 | zh_TW |
| dc.title | SALT HYPERSENSITIVE MUTANT 9, a nucleolar APUM23 protein, is essential for salt sensitivity in association with the ABA signaling pathway in Arabidopsis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.coadvisor | 葉開溫(Kai-Wun Yeh),張英?(Ing-Feng Chang) | |
| dc.contributor.oralexamcommittee | 張孟基(Men-Chi Chang),洪傳揚(Chwan-Yang Hong) | |
| dc.subject.keyword | 離層酸,SAHY9,APUM23,核仁,轉錄體,蛋白質體,鹽類逆境, | zh_TW |
| dc.subject.keyword | ABA,APUM23,nucleolus,proteome,salt stress,transcriptome, | en |
| dc.relation.page | 75 | |
| dc.identifier.doi | 10.6342/NTU201800659 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-02-27 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-107-1.pdf 未授權公開取用 | 5.48 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。