Please use this identifier to cite or link to this item:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69464
Title: | 探討皿培式牛樟芝萃取物在抗腫瘤和免疫調節的功效 Investigation of anti-tumor and immunomodulatory effects of dish-cultured fruiting body extract of Antrodia cinnamomea |
Authors: | Hsiang-Yi Hsu 徐向毅 |
Advisor: | 沈湯龍(Tang-Long Shen) |
Keyword: | 皿培式牛樟芝,子實體萃取物,腫瘤抑制,免疫調節, Dish-cultured Antrodia cinnamomea, fruiting body extract,anti-tumor,immunomodulation, |
Publication Year : | 2018 |
Degree: | 碩士 |
Abstract: | Antrodia cinnamomea (Syn. Taiwanofungus camphoratus and A. camphorata), an endemic medical fungus of Taiwan, grows on the inside of a tree hollow of the endemic broad-leaved tree, Cinnamomum kanehirae Hayata. Antrodia cinnamomea bears a number of bioactive compounds that exhibit beneficial effects to human health, such as immunomodulation, liver protection, and anti-cancer activity. Here, the dish-cultured A. cinnamomea fruiting body was subjected to analyze its morphological characteristics and major compounds. Moreover, the ethanol-extract of the dish-cultured A. cinnamomea fruiting body inhibited the in vitro growth of various human cancer cell lines, including liver cancer cell (Hep G2), breast carcinoma cell (MDA-MB-231, SkBr3 and MCF-7), lung cancer (H-1975, H-1650, H-292, A549), and neuroblastoma SK-N-SH. Nonetheless, the dish-cultured A. cinnamomea fruiting body extract did not show apparent toxicity to normal cell line, H9C2 a myoblast cell line. Furthermore, the in vivo study using nude mice showed that the oral administration of the dish-cultured A. cinnamomea fruiting body extract enables modulating the immune system as a result of increases in cytokine secretion, M1 macrophage (CD86+ CD11b+) activity, B cell (CD19+) population, and dendritic cells (CD11c+), whereas a decrease in M2 macrophage (CD206+ CD11b+) polarity, by which the dish-cultured A. cinnamomea fruiting body extract confers to its anti-tumor activity. This study implicated that the anti-tumor effect of the dish-cultured A. cinnamomea fruiting body is attributed by it immunomodulatory activities. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69464 |
DOI: | 10.6342/NTU201801279 |
Fulltext Rights: | 有償授權 |
Appears in Collections: | 植物病理與微生物學系 |
Files in This Item:
File | Size | Format | |
---|---|---|---|
ntu-107-1.pdf Restricted Access | 3.22 MB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.