探討皿培式牛樟芝萃取物在抗腫瘤和免疫調節的功效

Abstract

Antrodia cinnamomea (Syn. Taiwanofungus camphoratus and A. camphorata), an endemic medical fungus of Taiwan, grows on the inside of a tree hollow of the endemic broad-leaved tree, Cinnamomum kanehirae Hayata. Antrodia cinnamomea bears a number of bioactive compounds that exhibit beneficial effects to human health, such as immunomodulation, liver protection, and anti-cancer activity. Here, the dish-cultured A. cinnamomea fruiting body was subjected to analyze its morphological characteristics and major compounds. Moreover, the ethanol-extract of the dish-cultured A. cinnamomea fruiting body inhibited the in vitro growth of various human cancer cell lines, including liver cancer cell (Hep G2), breast carcinoma cell (MDA-MB-231, SkBr3 and MCF-7), lung cancer (H-1975, H-1650, H-292, A549), and neuroblastoma SK-N-SH. Nonetheless, the dish-cultured A. cinnamomea fruiting body extract did not show apparent toxicity to normal cell line, H9C2 a myoblast cell line. Furthermore, the in vivo study using nude mice showed that the oral administration of the dish-cultured A. cinnamomea fruiting body extract enables modulating the immune system as a result of increases in cytokine secretion, M1 macrophage (CD86+ CD11b+) activity, B cell (CD19+) population, and dendritic cells (CD11c+), whereas a decrease in M2 macrophage (CD206+ CD11b+) polarity, by which the dish-cultured A. cinnamomea fruiting body extract confers to its anti-tumor activity. This study implicated that the anti-tumor effect of the dish-cultured A. cinnamomea fruiting body is attributed by it immunomodulatory activities.