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標題: | 以石墨烯場效電晶體生物感測器研究病毒蛋白R和脂雙層之間的關係 The Study of Viral protein R and Lipid-Bilayer Interaction by Graphene Field-Effect Transistor Biosensors |
作者: | PEIBIN ZHONG 鐘沛彬 |
指導教授: | 陳逸聰(Yit-Tsong Chen) |
關鍵字: | 人類免疫缺乏病毒,石墨烯場效電晶體,支撐性磷脂雙層膜,病毒蛋白R,電壓依賴性陰離子通道蛋白, HIV-1,G-FET,SLB,Vpr,hVDAC-1, |
出版年 : | 2020 |
學位: | 碩士 |
摘要: | 俗稱艾滋病的後天免疫缺乏症候群(acquired immune deficiency syndrome, AIDS)是一種廣泛傳播的傳染病。造成該病的病原為人類免疫缺乏病毒(human immunodeficiency virus 1, HIV-1)。該病毒以人類免疫系統中的輔助性T細胞為攻擊目標,並最後使得人體的免疫系統逐漸喪失功能,而使患者死於感染或癌症。在HIV-1感染宿主的過程中,會產生一個名為病毒蛋白R (Viral protein R, Vpr)的附屬蛋白。該蛋白可誘發粒腺體提前發生細胞凋亡機制(apoptosis),有研究表明Vpr會與粒腺體外膜上的人類電壓依賴性陰離子通道(human voltage-dependent anion channel 1, hVDAC-1)作用,但具體的機制仍然不清楚。 石墨烯的高化學穩定性、高載子遷移率、高導電性等特性使其在生物感測方面備受關注,石墨烯的二維結構也可以給磷脂雙層膜提供穩定支持。因此本研究使用單層石墨烯作為通道,在矽基板上製作場效電晶體,接著在元件表面以囊泡融合法(vesicle fusion method)鋪上含有不同成分的支撐性脂雙層膜(supported-lipid bilayer, SLB),並量測由Vpr與SLB之間的交互作用引起的訊號變化。本研究中使用的SLB成分有中性磷脂DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine)、帶負電磷脂DOPG (1,2-dioleoyl-sn-glycero-3-phospho -(1'-rac-glycerol))和hVDAC-1。我們發現,當SLB中含有DOPG或hVDAC-1時,石墨烯場效電晶體(graphene field-effect transistor, G-FET)會表現出相當明顯的訊號。G-FET在未來進一步研究蛋白質與磷脂膜之間的關係時,將會是很好的量測工具。 Acquired immune deficiency syndrome, also called AIDS, a widely spread infectious disease, is caused by human immunodeficiency virus 1 (HIV-1). HIV-1 aims at T helper cells and finally destroys human immune system. Hence the patients will suffer from other infection diseases or cancers. HIV-1 usually produces an accessory protein, called Viral protein R (Vpr), which has shown to play a crucial role in HIV-1 pathogenicity, including triggering apoptosis. There have been some reports regarding the Vpr-hVDAC-1 interactions, but the detailed mechanisms remain unclear. Graphene has shown a great potential to be used as a biosensing material because of its high chemical stability, high carrier mobility, high conductivity, etc. Due to its two-dimensional (2D) property, graphene can provide a large, stable interface to support lipid bilayers. In this study, we fabricated a graphene field-effect transistor (G-FET) on silicon wafer and then modified the G-FET with a supported-lipid bilayer (SLB). The SLB consisted of different components and was paved on the G-FET (referred to as SLB/G-FET) via a vesicle fusion method. The signals induced by the Vpr-SLB interaction were recorded by the SLB/G-FET biosensor. Different components of the SLB were used in this study, including neutral phospholipid DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), negative charged lipid DOPG (1,2-dioleoyl-sn-glycero-3-phosphoglycerol) and hVDAC-1. Apparent signals were observed only when Vpr interacted with the SLB composed of DOPG or hVDAC-1. In conclusion, G-FET can serve as excellent platform for the investigation into the membrane proteins-related interactions. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68860 |
DOI: | 10.6342/NTU202003665 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學系 |
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