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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳逸聰(Yit-Tsong Chen) | |
dc.contributor.author | PEIBIN ZHONG | en |
dc.contributor.author | 鐘沛彬 | zh_TW |
dc.date.accessioned | 2021-06-17T02:39:02Z | - |
dc.date.available | 2022-08-17 | |
dc.date.copyright | 2020-08-24 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-08-17 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68860 | - |
dc.description.abstract | 俗稱艾滋病的後天免疫缺乏症候群(acquired immune deficiency syndrome, AIDS)是一種廣泛傳播的傳染病。造成該病的病原為人類免疫缺乏病毒(human immunodeficiency virus 1, HIV-1)。該病毒以人類免疫系統中的輔助性T細胞為攻擊目標,並最後使得人體的免疫系統逐漸喪失功能,而使患者死於感染或癌症。在HIV-1感染宿主的過程中,會產生一個名為病毒蛋白R (Viral protein R, Vpr)的附屬蛋白。該蛋白可誘發粒腺體提前發生細胞凋亡機制(apoptosis),有研究表明Vpr會與粒腺體外膜上的人類電壓依賴性陰離子通道(human voltage-dependent anion channel 1, hVDAC-1)作用,但具體的機制仍然不清楚。 石墨烯的高化學穩定性、高載子遷移率、高導電性等特性使其在生物感測方面備受關注,石墨烯的二維結構也可以給磷脂雙層膜提供穩定支持。因此本研究使用單層石墨烯作為通道,在矽基板上製作場效電晶體,接著在元件表面以囊泡融合法(vesicle fusion method)鋪上含有不同成分的支撐性脂雙層膜(supported-lipid bilayer, SLB),並量測由Vpr與SLB之間的交互作用引起的訊號變化。本研究中使用的SLB成分有中性磷脂DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine)、帶負電磷脂DOPG (1,2-dioleoyl-sn-glycero-3-phospho -(1'-rac-glycerol))和hVDAC-1。我們發現,當SLB中含有DOPG或hVDAC-1時,石墨烯場效電晶體(graphene field-effect transistor, G-FET)會表現出相當明顯的訊號。G-FET在未來進一步研究蛋白質與磷脂膜之間的關係時,將會是很好的量測工具。 | zh_TW |
dc.description.abstract | Acquired immune deficiency syndrome, also called AIDS, a widely spread infectious disease, is caused by human immunodeficiency virus 1 (HIV-1). HIV-1 aims at T helper cells and finally destroys human immune system. Hence the patients will suffer from other infection diseases or cancers. HIV-1 usually produces an accessory protein, called Viral protein R (Vpr), which has shown to play a crucial role in HIV-1 pathogenicity, including triggering apoptosis. There have been some reports regarding the Vpr-hVDAC-1 interactions, but the detailed mechanisms remain unclear. Graphene has shown a great potential to be used as a biosensing material because of its high chemical stability, high carrier mobility, high conductivity, etc. Due to its two-dimensional (2D) property, graphene can provide a large, stable interface to support lipid bilayers. In this study, we fabricated a graphene field-effect transistor (G-FET) on silicon wafer and then modified the G-FET with a supported-lipid bilayer (SLB). The SLB consisted of different components and was paved on the G-FET (referred to as SLB/G-FET) via a vesicle fusion method. The signals induced by the Vpr-SLB interaction were recorded by the SLB/G-FET biosensor. Different components of the SLB were used in this study, including neutral phospholipid DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), negative charged lipid DOPG (1,2-dioleoyl-sn-glycero-3-phosphoglycerol) and hVDAC-1. Apparent signals were observed only when Vpr interacted with the SLB composed of DOPG or hVDAC-1. In conclusion, G-FET can serve as excellent platform for the investigation into the membrane proteins-related interactions. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T02:39:02Z (GMT). No. of bitstreams: 1 U0001-1708202003210200.pdf: 3344911 bytes, checksum: 0d5cb5a04696d6eeb705a9ff0ba14531 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 中文摘要 i ABSTRACT ii 目錄 iv 圖目錄 viii 表目錄 xiii 簡稱用語對照表 xiv 第1章 導論 1 1.1 石墨烯簡介 1 1.2 場效電晶體生物感測器 2 1.3 磷脂雙層膜介紹 3 1.4 研究動機 5 第2章 文獻回顧 9 2.1 石墨烯之結構與特性 9 2.2 石墨烯之製備方法 11 2.2.1 機械剝離法 11 2.2.2 氧化石墨烯還原法 12 2.2.3 碳化矽磊晶成長法 14 2.2.4 化學氣相沉積法合成 14 2.2.5 常見的石墨烯製備方法之比較 16 2.3 G-FET感測器 16 2.4 支撐性脂雙層膜 21 2.5 病毒蛋白R 24 2.6 電壓依賴性陰離子通道蛋白 27 第3章 實驗方法與材料 29 3.1 G-FET製作 29 3.1.1 裁切清洗矽基板 29 3.1.2 轉置石墨烯 29 3.1.3 縮小石墨烯尺寸 31 3.1.4 以黃光顯影及真空蒸鍍製作金屬電極 32 3.1.5 以原子層沉積技術製作氧化層 33 3.1.6 清洗晶片上的光阻及其上的金屬和氧化層 34 3.1.7 使用打線機延長電極 35 3.2 生物樣品量測平台 36 3.2.1 聚二甲基矽氧烷溶液槽製備 36 3.2.2 緩衝溶液的製備 36 3.2.3 脂質體製備 37 3.3 Vpr與SLB交互作用之實驗流程 39 3.3.1 SLB之鋪放 39 3.3.2 Vpr與SLB發生作用 40 3.4 檢測之實驗儀器 41 3.4.1 光學金相顯微鏡 41 3.4.2 原子力顯微鏡 41 3.4.3 鎖相放大器 42 第4章 結果與討論 44 4.1 Liposome粒徑檢測 44 4.2 漏電檢測 45 4.3 Vpr與SLB之測量 46 4.3.1 控制組實驗 46 4.3.1.1 TEV蛋白酶與石墨烯之交互作用 46 4.3.1.2 TEV蛋白酶與DOPC/DOPG之交互作用 47 4.3.2 Vpr與DOPC之交互作用 48 4.3.3 Vpr與DOPC/DOPG之交互作用 49 4.3.3.1 Vpr與DOPC/DOPG(1/1)之交互作用 49 4.3.3.2 Vpr與100% DOPG以及DOPC/DOPG (95/5)之交互作用 53 4.3.4 Vpr與DOPC/hVDAC-1之交互作用 54 第5章 結論 57 參考文獻 59 | |
dc.language.iso | zh-TW | |
dc.title | 以石墨烯場效電晶體生物感測器研究病毒蛋白R和脂雙層之間的關係 | zh_TW |
dc.title | The Study of Viral protein R and Lipid-Bilayer Interaction by Graphene Field-Effect Transistor Biosensors | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 余慈顏(Tsyr-Yan Yu),潘建源(Chien-Yuan Pan) | |
dc.subject.keyword | 人類免疫缺乏病毒,石墨烯場效電晶體,支撐性磷脂雙層膜,病毒蛋白R,電壓依賴性陰離子通道蛋白, | zh_TW |
dc.subject.keyword | HIV-1,G-FET,SLB,Vpr,hVDAC-1, | en |
dc.relation.page | 64 | |
dc.identifier.doi | 10.6342/NTU202003665 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-08-18 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 化學研究所 | zh_TW |
顯示於系所單位: | 化學系 |
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