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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔣丙煌(Been-Huang Chiang) | |
| dc.contributor.author | Jia-Ling Li | en |
| dc.contributor.author | 李佳玲 | zh_TW |
| dc.date.accessioned | 2021-06-17T00:34:58Z | - |
| dc.date.available | 2012-03-19 | |
| dc.date.copyright | 2012-03-19 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-02-06 | |
| dc.identifier.citation | 1. 陳駿季、蕭吉雄，植物組織培養技術在健康種苗生產之應用，農業新世紀：農業生物科技園區專訊，5 民95.07，頁9-13。
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A.; Kroymann, J., Metabolic Engineering in Nicotiana benthamiana Reveals Key Enzyme Functions in Arabidopsis Indole Glucosinolate Modification. Plant Cell 2011, 23, 716-729. 55. Preil, W.; Florek, P.; Wix, U.; Beck, A.,Towards mass propagation by use of bioreactors. Acta Horticulturae. 1988, 226, 99-106 56. Rangkadilok, N.; Nicolas, M. E.; Bennett, R. N.; Eagling, D. R.; Premier, R. R.; Taylor, P. W. J., The effect of sulfur fertilizer on glucoraphanin levels in broccoli (B-oleracea L. var. italica) at different growth stages. J. Agric. Food Chem.2004, 52, 2632-2639. 57. Rouzaud, G.; Young, S. A.; Duncan, A. J., Hydrolysis of glucosinolates to isothiocyanates after ingestion of raw or microwaved cabbage by human volunteers. Cancer Epidemiol. Biomarkers Prev. 2004, 13, 125-131. 58. Rychlik, M.; Adam, S. T., Glucosinolate and folate content in sprouted broccoli seeds. Eur. Food Res. Technol. 2008, 226, 1057-1064. 59. Schlatmann, J. E.; Vinke, J. L.; Tenhoopen, H. J. G.; Heijnen, J. 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Y., Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: Manipulation of medium sucrose. J. Biotechnol. 1996, 51, 49-56. 70. Zimmermann, N. S.; Gerendas, J.; Krumbein, A., Identification of desulphoglucosinolates in Brassicaceae by LC/MS/MS: Comparison of ESI and atmospheric pressure chemical ionisation-MS. Mol. Nutr. Food Res. 2007, 51, 1537-1546. 71. Ziv, M., Bioreactor Technology for Plant Micropropagation. Horticultural Reviews 2002, 24, 1-30 72. Ziv, M.; Hadar, A., Morphogenic pattern of Nephrolepsis exaltata Bostoniensis in agar-gelled or liquid culture. Israel J. Bot. 1991, 40, 7-16 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66421 | - |
| dc.description.abstract | 十字花科植物廣受大家喜愛，其所含有的二次代謝產物－硫代葡萄糖苷，已被許多文獻證實具有許多生理活性，如抑制腫瘤形成、抗氧化與提高人體中解毒酵素的活性等。本研究以十字花科中之青花菜為材料，希望以植物組織培養的方式來生產硫代葡萄糖苷。實驗分三階段進行。第一階段乃尋找適合青花菜形成癒傷組織的培養基。在嘗試不同濃度與種類的植物荷爾蒙後，發現以MS培養基添加1 mg/ L 2,4-D與0.5 mg/ L的BA有最好的誘導效果，成功誘導的癒傷組織高達80%。第二階段進行搖瓶培養，發現在七天後，glucobrassicin會增加。第三階段則以發酵槽放大培養，結果顯示硫代葡萄糖苷在癒傷組織內含量較多，僅有少量硫代葡萄糖苷會釋放到培養液中。其中又以氣舉式發酵槽通入5 %二氧化碳下，其癒傷組織中的硫代葡萄糖苷含量最多。而利用發酵槽培養癒傷組織，其水萃液(1 g/ 30 mL H2O)抗氧化能力又以氣舉式發酵槽不通入二氧化碳最好，其也含有較高的酚類物質。在癒傷組織中硫代葡萄糖苷之HPLC分析圖譜中，在滯留時間為27分鐘有一明顯波峰，經由質譜儀分析後，推測其結構可能為4-Methoxyglucobrassicin。 | zh_TW |
| dc.description.abstract | Brassicaceae family is popular vegetable around the world, and it contains secondary metabolites-glucosinolates, which are potent source of protective chemicals against cancer. Glucosinolates can induce the phase II enzyme system thus inhibiting the growth of tumor.
The purpose of our efforts was to produce glucosinolates by plant tissue culture, and we chose broccoli, one of the important vegetables in Brassicaceae family, as material. The experiment was divided into three stages. Firstly, we tried to find out an optimal medium to induce broccoli to form callus. After investigating different kinds and concentrations of plant hormones, we found that addition of 1 g/ mL 2,4-D and 0.5 g/mL BA in the MS medium could obtain the best result. The percentage of health callus was above 80. In the second stage, shaker flask was used to cultivate broccoli callus in suspension culture. It was found that the glucobrassicin increased after 7 days of cultivation. Finally, fermentor was used to cultivate broccoli callus. We found that glucosinolates were mostly in the callus, and only a little released to the medium. The content of glucosinolates in callus cultivated by air lift fermentor with 5 % CO2 was the highest. However, the callus with the highest antioxidant activity was obtained in air lift fermentor without CO2, and it also had the highest phenolics content. The HPLC profile revealed that the major glucosinolates appeared at retention time 27 min, and MS analysis indicated that the compound is 4-Methoxyglucobrassicin. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T00:34:58Z (GMT). No. of bitstreams: 1 ntu-101-R98641015-1.pdf: 2062138 bytes, checksum: 3da873936d758e8304bbf8f4dfe92d1e (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 謝誌 Ⅰ
摘要 Ⅱ Abstract Ⅲ 目錄 Ⅳ 圖目錄 Ⅷ 表目錄 Ⅹ 縮寫表 XI 第壹章 文獻回顧 1 第一節 植物二次代謝產物 1 (一). 二次代謝物產生機制 1 (二). 二次代謝產物與癌症 4 第二節 十字花科植物 5 (一). 青花菜 5 (二). 硫代葡萄糖苷(Glucosinolates) 5 A. 分佈 7 B. 生理活性 10 第三節 植物組織培養 11 (一). 原理與應用 11 (二). 培養基組成 11 A. 基礎培養基 11 B. 生長調節劑 13 C. 凝膠物質 14 D. 其他 14 (三). 培植體的選擇 14 (四). 培養環境 15 A. pH值 15 B. 培養基選擇 15 C. 光照 15 D. 溫度 15 E. 震盪速率 16 第四節 生物反應器培養二次代謝產物 17 (一). 剪應力 18 (二). 通氣速率 18 (三). 氣相組成 19 A. 氧氣(O2) 19 B. 二氧化碳(CO2) 20 C. 乙烯(C2H4) 20 第五節 生化反應器介紹 20 (一). 攪拌槽式反應器(Stirred Tank Reactor) 21 (二). 氣泡塔式反應器(Bubble Column Reactor) 21 (三). 氣舉式反應器(Airlift Reactor) 22 (四). 改良式氣舉式反應器(Modified Airlift Reactor) 22 第貳章 研究目的與實驗架構 25 (一). 研究目的 25 (二). 實驗架構 26 A. 以不同的植物荷爾蒙來誘導癒傷組織形成 26 B. 將癒傷組織進入液態培養階段－搖瓶培養 27 C. 發酵槽放大培養 28 第參章 材料與方法 29 (一). 培養基配置 29 A. 播種用培養基 29 B. 誘導癒傷組織培養基 29 (二). 無菌播種 30 (三). 誘導癒傷組織形成 31 (四). 硫代葡萄糖苷分析 31 (五). 以發酵槽培養癒傷組織 33 (六). 營養源分析 34 A. 碳源分析 34 B. 氮源分析 36 C. 磷源分析 38 (七). 抗氧化能力測定 42 A. 清除DPPH自由基能力 42 B. 總抗氧化能力 43 (八). 總酚含量測定 44 (九). 總類黃酮含量測定 44 (十). 質譜分析 45 第肆章 結果與討論 47 A. 以不同的植物荷爾蒙來誘導癒傷組織形成 47 B. 將癒傷組織進入液態培養階段－搖瓶培養 53 C. 發酵槽放大培養 55 一、 pH值變化情形 55 二、 醣類變化 56 三、 Ammonium(NH4+)消耗情形 56 四、 Nitrate(NO3-)消耗情形 57 五、 Phosphorus(PO43-)消耗情形 58 六、 硫代葡萄糖苷分析 65 七、 質譜分析 74 八、 抗氧化能力分析 79 九、 總酚含量分析 79 十、 總類黃酮含量分析 79 第伍章 結論 81 第陸章 參考文獻 83 | |
| dc.language.iso | zh-TW | |
| dc.subject | 硫代葡萄糖苷 | zh_TW |
| dc.subject | 癒傷組織 | zh_TW |
| dc.subject | 二次代謝物 | zh_TW |
| dc.subject | 青花菜 | zh_TW |
| dc.subject | 發酵槽 | zh_TW |
| dc.subject | broccoli | en |
| dc.subject | callus | en |
| dc.subject | secondary metabolites | en |
| dc.subject | fermentor | en |
| dc.subject | glucosinolates | en |
| dc.title | 利用青花菜組織培養生產硫代葡萄糖苷之研究 | zh_TW |
| dc.title | Production of glucosinolates by tissue culture of
Brassica oleracea var. italica | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李敏雄,黃鵬林 | |
| dc.subject.keyword | 青花菜,癒傷組織,二次代謝物,發酵槽,硫代葡萄糖苷, | zh_TW |
| dc.subject.keyword | broccoli,callus,secondary metabolites,fermentor,glucosinolates, | en |
| dc.relation.page | 90 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-02-07 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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| ntu-101-1.pdf 未授權公開取用 | 2.01 MB | Adobe PDF |
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