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Title: | 檳榔子萃取多酚於小鼠食物過敏模式中之免疫調節作用 The immunomodulatory effect of polyphenol-enriched areca nut extract in a murine model of food allergy |
Authors: | Yu-Ju Lin 林玉茹 |
Advisor: | 詹東榮 |
Keyword: | 檳榔,食物過敏,免疫球蛋白IgE,骨髓衍生性抑制細胞,多酚,幫手型T細胞, areca nut,food allergy,IgE,mast cell,myeloid-derived suppressor cell,polyphenols,T helper cell, |
Publication Year : | 2012 |
Degree: | 碩士 |
Abstract: | 傳統中醫學以檳榔片治療腸道寄生蟲,緩解下痢及裡急後重等症狀,但目前僅少許科學證據支持其藥理作用。本實驗室先前研究指出檳榔子萃取物(areca-nut extract;ANE)會調節遲發型過敏反應,顯示T細胞免疫反應受到檳榔子成分的影響,且ANE增加表現CD11b及Gr-1的骨髓衍生性抑制細胞(myeloid-derived suppressor cells;MDSC)。本實驗進一步探討檳榔子萃取多酚(polyphenol-enriched ANE;PANE)對食物過敏的免疫調節作用。腹腔注射組於第1-3、15-17及29-31天給予BALB/c小鼠腹腔注射PANE(5-25 mg/kg/day溶於無菌生理食鹽水;0.1 mL/mouse),共9劑;口服組則飲水給予PANE(0.05%和0.1% w/v;大約5 mL/mouse/day)。小鼠在第3及17天分別以卵白蛋白(ovalbumin;OVA)免疫,並在第31-49天每二天管餵一次OVA(50 mg溶於0.3 mL生理食鹽水)以誘發過敏性下痢。結果顯示PANE不影響小鼠的體重、脾臟指數和脾臟的細胞組成比例。腹腔注射和口服給予PANE皆抑制食物過敏反應,包括下痢發生率、腸道發炎和肥胖細胞的浸潤及去顆粒化。PANE降低腸道中IL-4陽性細胞,並增加分泌IL-10的Gr-1陽性細胞數量。此外,腹腔注射PANE減少腸道中IFN-γ陽性細胞,也抑制血清中IgE及OVA專一性IgG1的生成。上述結果顯示PANE的抗過敏性下痢是藉由抑制T細胞媒介的免疫反應,而其作用機轉可能與誘導骨髓衍生性抑制細胞有關。 Arecae semen, the slice of dried areca nut, has been used in traditional Chinese medicine for the treatment of intestinal parasitosis, diarrhea and tenesmus. However, limited scientific evidence is available to substantiate its pharmacological effects. Previous studies from this laboratory showed that areca nut extract (ANE) influenced delayed-type hypersensitivity reactions, suggesting that the T-cell mediated immunity was affected by areca ingredients. In addition, ANE induced the generation of myeloid-derived suppressor cells, characterized as CD11b+Gr-1+ cells. This thesis study investigated the immunomodulatory effect of polyphenol-enriched ANE (PANE) in a murine model of food allergy. For intraperitoneal (IP) administration, BALB/c mice were daily administered with PANE (5-25 mg/kg in sterile saline; 0.1 mL/mouse) from day 1-3, day 15-17 and day 29-31. For oral administration, mice were administrated with 0.05% and 0.1% (w/v; approximate 5 mL/mouse/day) of PANE in drinking water throughout the entire experiment. Except for the naive and non-sensitized group, the mice were systemically sensitized with ovalbumin (OVA) on day 3 and day 17. Except for the NA group, mice were repeatedly challenged with OVA (50 mg/0.3 mL in saline/mouse) by gavage from day 31 to 49 to induce allergic responses. Administration of PANE did not affect the body weight, spleen index, and the cellularity of splenocytes. Both IP injection and oral uptake of PANE attenuated food allergic responses, including diarrhea incidence, infiltration and degranulation of mast cells and the inflammatory pathological change in the intestine. PANE significantly reduced the number of IL-4+ cells in the duodenum, but dose-dependently increased the infiltration of IL-10-producing Gr-1+ cells. Furthermore, the number of IFN-γ+ cells in the duodenum and the serum production of total IgE and OVA-IgG1 were attenuated by IP treatment with PANE. Collectively, these results demonstrated the anti-allergic effect of PANE, which is associated with the suppression of T cell-mediated immunity and the induction of MDSC generation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66255 |
Fulltext Rights: | 有償授權 |
Appears in Collections: | 獸醫學系 |
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