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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔣丙煌(Been-Huang Chiang) | |
| dc.contributor.author | Yi-Chien Fu | en |
| dc.contributor.author | 傅一茜 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:14:16Z | - |
| dc.date.available | 2020-08-17 | |
| dc.date.copyright | 2012-08-27 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2012-08-18 | |
| dc.identifier.citation | 台灣地區 2009 年主要死因統計(2010)。取自：行政院衛生署衛生統計資訊網。http://www.doh.gov.tw/
王照元 大腸直腸癌的輔助性化學治療. 中華民國癌症醫學會雜誌 2008, 24, 180-184. Abdulkadir, S. A.; Carvalhal, G. F.; Kaleem, Z.; Kisiel, W.; Humphrey, P. A.; Catalona, W. J.; Milbrandt, J. Tissue factor expression and angiogenesisin human prostate carcinoma. Hum. Pathol. 2000, 31, 443-447. Afanas' ev, I. B.; Dcrozhko, A. I.; Brodskii, A. V.; Kostyuk, V. A.; Potapovitch, A. I. Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation. Biochem. Pharmacol. 1989, 38, 1763-1769. Aggarwal, B. B.; Kumar, A.; Bharti, A. C. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res 2003, 23, 363-398. Aggarwal, B. B.; Shishodia, S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem. Pharmacol. 2006, 71, 1397-1421. Ahmed, F. E. Effect of diet, life style, and other environmental/chemopreventive factors on colorectal cancer development, and assessment of the risks. J. Environ. Sci. Health Pt. C 2004, 22, 91-148. Araujo, J. R.; Gonįalves, P.; Martel, F. Chemopreventive effect of dietary polyphenols in colorectal cancer cell lines. Nutr. Res. 2011, 31, 77-87. Aviram, M.; Rosenblat, M.; Gaitini, D.; Nitecki, S.; Hoffman, A.; Dornfeld, L.; Volkova, N.; Presser, D.; Attias, J.; Liker, H. Pomegranate juice consumption for 3 years by patients with carotid artery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation. Clin. Nutr. 2004, 23, 423-433. Banibrata, S.; Faye, M. Regulation of Src Family Kinases in Human Cancers. J. Recept. Signal Transduct. 2011, 2011, 1-14. Barve, A.; Khor, T. O.; Hao, X.; Keum, Y. S.; Yang, C. S.; Reddy, B.; Kong, A. N. T. Murine prostate cancer inhibition by dietary phytochemicals—curcumin and phenyethylisothiocyanate. Pharm. Res. 2008, 25, 2181-2189. Boyden, S. V. Cellular discrimination between indigenous and foreign matter. J. Theor. Biol. 1962, 3, 123-131. Brun, R.; Naroditsky, I.; Waterman, M.; Ben-Izhak, O.; Groisman, G.; Ilan, N.; Vlodavsky, I. Heparanase expression by Barrett's epithelium and during esophageal carcinoma progression. Mod. Pathol. 2009, 22, 1548-1554. Chen, J. J. W.; Lin, Y. C.; Yao, P. L.; Yuan, A.; Chen, H. Y.; Shun, C. T.; Tsai, M. F.; Chen, C. H.; Yang, P. C. Tumor-associated macrophages: the double-edged sword in cancer progression. J. Clin. Oncol. 2005, 23, 953-964. Chun, K. S.; Keum, Y. S.; Han, S. S.; Song, Y. S.; Kim, S. H.; Surh, Y. J. Curcumin inhibits phorbol ester-induced expression of cyclooxygenase-2 in mouse skin through suppression of extracellular signal-regulated kinase activity and NF-κB activation. Carcinogenesis 2003, 24, 1515-1524. Cohen, I.; Maly, B.; Simon, I.; Meirovitz, A.; Pikarsky, E.; Zcharia, E.; Peretz, T.; Vlodavsky, I.; Elkin, M. Tamoxifen Induces Heparanase Expression in Estrogen Receptor–Positive Breast Cancer. Clin. Cancer Res. 2007, 13, 4069-4077. Collett, G. P.; Campbell, F. C. Curcumin induces c-jun N-terminal kinase-dependent apoptosis in HCT116 human colon cancer cells. Carcinogenesis 2004, 25, 2183-2189. Condeelis, J.; Pollard, J. W. Macrophages: obligate partners for tumor cell migration, invasion, and metastasis. Cell 2006, 124, 263-266. Crowther, M.; Brown, N.; Bishop, E.; Lewis, C. Microenvironmental influence on macrophage regulation of angiogenesis in wounds and malignant tumors. J. Leukoc. Biol. 2001, 70, 478-490. Cunningham, D.; Zalcberg, J.; Rath, U.; Oliver, I.; Van Cutsem, E.; Svensson, C.; Seitz, J.; Harper, P.; Kerr, D.; Perez-Manga, G. Final results of a randomised trial comparing ‘Tomudex’(raltitrexed) with 5-fluorouracil plus leucovorin in advanced colorectal cancer. Ann. Oncol. 1996, 7, 961-965. Cunningham, D.; Humblet, Y.; Siena, S.; Khayat, D.; Bleiberg, H.; Santoro, A.; Bets, D.; Mueser, M.; Harstrick, A.; Verslype, C. Cetuximab monotherapy and cetuximab plus irinotecan in irinotecan-refractory metastatic colorectal cancer. N. Engl. J. Med. 2004, 351, 337-345. Daigneault, M.; Preston, J. A.; Marriott, H. M.; Whyte, M. K. B.; Dockrell, D. H. The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. PLoS One 2010, 5, e8668. Daniel, S.; Limson, J. L.; Dairam, A.; Watkins, G. M.; Daya, S. Through metal binding, curcumin protects against lead-and cadmium-induced lipid peroxidation in rat brain homogenates and against lead-induced tissue damage in rat brain. J. Inorg. Biochem. 2004, 98, 266-275. Dong, Z.; Yoneda, J.; Kumar, R.; Fidler, I. J. Angiostatin-mediated Suppression of Cancer Metastases by Primary Neoplasms Engineered to Produce Granulocyte/Macrophage Colony–stimulating Factor. J. Exp. Med. 1998, 188, 755-763. Engvall, E.; Perlmann, P. Enzyme-linked immunosorbent assay, ELISA. J. Immunol. 1972, 109, 129. Ferro, V.; Hammond, E.; Fairweather, J. K. The development of inhibitors of heparanase, a key enzyme involved in tumour metastasis, angiogenesis and inflammation. Med. Chem. 2004, 4, 693-702. Folkman, J.; Shing, Y. Control of angiogenesis by heparin and other sulfated polysaccharides. Adv.Exp.Med.Biol. 1992, 313, 355-364. Fulda, S.; Debatin, K. M. Sensitization for tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by the chemopreventive agent resveratrol. Cancer Res. 2004, 64, 337. Granado‐Serrano, A. B.; Angeles Martin, M.; Bravo, L.; Goya, L.; Ramos, S. Time‐course regulation of quercetin on cell survival/proliferation pathways in human hepatoma cells. Mol. Nutr. Food Res. 2008, 52, 457-464. Hagemann, T.; Robinson, S. C.; Schulz, M.; Trumper, L.; Balkwill, F. R.; Binder, C. Enhanced invasiveness of breast cancer cell lines upon co-cultivation with macrophages is due to TNF-α dependent up-regulation of matrix metalloproteases. Carcinogenesis 2004, 25, 1543-1549. Hanada, T.; Nakagawa, M.; Emoto, A.; Nomura, T.; Nasu, N.; Nomura, Y. Prognostic value of tumor‐associated macrophage count in human bladder cancer. Int. J. Urol. 2000, 7, 263-269. Hiratsuka, S.; Nakamura, K.; Iwai, S.; Murakami, M.; Itoh, T.; Kijima, H.; Shipley, J. M.; Senior, R. M.; Shibuya, M. MMP9 induction by vascular endothelial growth factor receptor-1 is involved in lung-specific metastasis. Cancer cell 2002, 2, 289-300. Huang, M. T.; Lou, Y. R.; Xie, J. G.; Ma, W.; Lu, Y. P.; Yen, P.; Zhu, B. T.; Newmark, H.; Ho, C. T. Effect of dietary curcumin and dibenzoylmethane on formation of 7, 12-dimethylbenz anthracene-induced mammary tumors and lymphomas/leukemias in Sencar mice. Carcinogenesis 1998, 19, 1697-1700. Hurwitz, H.; Fehrenbacher, L.; Novotny, W.; Cartwright, T.; Hainsworth, J.; Heim, W.; Berlin, J.; Baron, A.; Griffing, S.; Holmgren, E. Bevacizumab plus irinotecan, fluorouracil, and leucovorin for metastatic colorectal cancer. N. Engl. J. Med. 2004, 350, 2335-2342. Ilan, N.; Elkin, M.; Vlodavsky, I. Regulation, function and clinical significance of heparanase in cancer metastasis and angiogenesis. Int. J. Biochem. Cell Biol. 2006, 38, 2018-2039. Ilyas, M.; Straub, J.; Tomlinson, I.; Bodmer, W. Genetic pathways in colorectal and other cancers1. Eur. J. Cancer 1999, 35, 1986-2002. J.M., C. Surgery for Rectal Cancer: The State of The Art. J. Chinese Oncol. Soc. 2008, 24-3, 167-171. JACKSON, K. M.; FRAZIER, M. C.; HARRIS, W. B. Suppression of androgen receptor expression by dibenzoylmethane as a therapeutic objective in advanced prostate cancer. Anticancer Res. 2007, 27, 1483-1488. Jakus, V. The role of free radicals, oxidative stress and antioxidant systems in diabetic vascular disease. Bratisl. Med. J. 2000, 101, 541-551. Janakiram, N. B.; Rao, C. V. Molecular markers and targets for colorectal cancer prevention. Acta Pharmacol. Sin. 2008, 29, 1-20. Jedinak, A.; Dudhgaonkar, S.; Sliva, D. Activated macrophages induce metastatic behavior of colon cancer cells. Immunobiology 2010, 215, 242-249. K.J., B. Heparanases: endoglycosidases that degrade heparan sulfate proteoglycans. Glycobiology 2001, 11, 91-98. Khan, N.; Mukhtar, H. Tea polyphenols for health promotion. Life Sci. 2007, 81, 519-533. Kim, W. K.; Bang, M. H.; Kim, E. S.; Kang, N. E.; Jung, K. C.; Cho, H. J.; Park, J. H. Y. Quercetin decreases the expression of ErbB2 and ErbB3 proteins in HT-29 human colon cancer cells. J. Nutr. Biochem. 2005, 16, 155-162. Kohro, T.; Tanaka, T.; Murakami, T.; Wada, Y.; Aburatani, H.; Hamakubo, T.; Kodama, T. A comparison of differences in the gene expression profiles of phorbol 12-myristate 13-acetate differentiated THP-1 cells and human monocyte-derived macrophage. J. Atheroscler. Thromb. 2004, 11, 88-97. Kotha, A.; Sekharam, M.; Cilenti, L.; Siddiquee, K.; Khaled, A.; Zervos, A. S.; Carter, B.; Turkson, J.; Jove, R. Resveratrol inhibits Src and Stat3 signaling and induces the apoptosis of malignant cells containing activated Stat3 protein. Mol. Cancer Ther. 2006, 5, 621. Lamagna, C.; Aurrand-Lions, M.; Imhof, B. A. Dual role of macrophages in tumor growth and angiogenesis. J. Leukoc. Biol. 2006, 80, 705-713. Leek, R. D.; Lewis, C. E.; Whitehouse, R.; Greenall, M.; Clarke, J.; Harris, A. L. Association of macrophage infiltration with angiogenesis and prognosis in invasive breast carcinoma. Cancer Res. 1996, 56, 4625-4629. Levy-Adam, F.; Feld, S.; Suss-Toby, E.; Vlodavsky, I.; Ilan, N. Heparanase facilitates cell adhesion and spreading by clustering of cell surface heparan sulfate proteoglycans. PLoS One 2008, 3, e2319. Levy, D. B.; Smith, K. J.; Beazer-Barclay, Y.; Hamilton, S. R.; Vogelstein, B.; Kinzler, K. W. Inactivation of both APC alleles in human and mouse tumors. Cancer Res. 1994, 54, 5953-5958. Lin, C. W.; Hou, W. C.; Shen, S. C.; Juan, S. H.; Ko, C. H.; Wang, L. M.; Chen, Y. C. Quercetin inhibition of tumor invasion via suppressing PKCδ/ERK/AP-1-dependent matrix metalloproteinase-9 activation in breast carcinoma cells. Carcinogenesis 2008, 29, 1807-1815. Loeb, L. A. Microsatellite instability: marker of a mutator phenotype in cancer. Cancer Res. 1994, 54, 5059-5063. Lynch, H. T.; Smyrk, T. C.; Lanspa, S. J.; Lynch, P. M.; Watson, P.; Strayhorn, P. C.; Bronson, E. K.; Lynch, J. F.; Priluck, I. A.; Appelman, H. D. Phenotypic variation in colorectal adenoma/cancer expression in two families. Hereditary flat adenoma syndrome. Cancer 1990, 66, 909-915. Maggiolini, M.; Bonofiglio, D.; Marsico, S.; Panno, M. L.; Cenni, B.; Picard, D.; Ando, S. Estrogen receptor α mediates the proliferative but not the cytotoxic dose-dependent effects of two major phytoestrogens on human breast cancer cells. Mol. Pharmacol. 2001, 60, 595-602. Mantovani, A.; Bottazzi, B.; Colotta, F.; Sozzani, S.; Ruco, L. The origin and function of tumor-associated macrophages. Immunology 1992, 13, 265-270. Mantovani, A.; Sozzani, S.; Locati, M.; Allavena, P.; Sica, A. Macrophage polarization: tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes. Trends Immunol. 2002, 23, 549-555. Mantovani, A.; Schioppa, T.; Porta, C.; Allavena, P.; Sica, A. Role of tumor-associated macrophages in tumor progression and invasion. Cancer Metastasis Rev. 2006, 25, 315-322. Mantovani, A.; Allavena, P.; Sica, A.; Balkwill, F. Cancer-related inflammation. Nature 2008, 454, 436-444. Masola, V.; Maran, C.; Tassone, E.; Zin, A.; Rosolen, A.; Onisto, M. Heparanase activity in alveolar and embryonal rhabdomyosarcoma: implications for tumor invasion. BMC cancer 2009, 9, 304. McKenzie, E. Heparanase: a target for drug discovery in cancer and inflammation. Br. J. Pharmacol. 2007, 151, 1-14. Merlo, L. M. F.; Pepper, J. W.; Reid, B. J.; Maley, C. C. Cancer as an evolutionary and ecological process. Nat. Rev. Cancer 2006, 6, 924-935. Mertens-Talcott, S. U.; Percival, S. S. Ellagic acid and quercetin interact synergistically with resveratrol in the induction of apoptosis and cause transient cell cycle arrest in human leukemia cells. Cancer Lett. 2005, 218, 141-151. Mohan, J.; Gandhi, A. A.; Bhavya, B. C.; Rashmi, R.; Karunagaran, D.; Indu, R.; Santhoshkumar, T. R. Caspase-2 triggers Bax-Bak-dependent and-independent cell death in colon cancer cells treated with resveratrol. J. Biol. Chem. 2006, 281, 17599-17611. Molina, M. F.; Sanchez-Reus, I.; Iglesias, I.; Benedi, J. Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver. Biol. Pharm. Bull. 2003, 26, 1398-1402. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55-63. Mukhtar, H.; Ahmad, N. Tea polyphenols: prevention of cancer and optimizing health. Am. J. Clin. Nutr. 2000, 71, 1698-1702. Nakagawa, H.; Kiyozuka, Y.; Uemura, Y.; Senzaki, H.; Shikata, N.; Hioki, K.; Tsubura, A. Resveratrol inhibits human breast cancer cell growth and may mitigate the effect of linoleic acid, a potent breast cancer cell stimulator. J. Cancer Res. Clin. Oncol. 2001, 127, 258-264. Nakajima, M.; Irimura, T.; Nicolson, G. L. Heparanases and tumor metastasis. Journal of cellular biochemistry 1988, 36, 157-167. Naparstek, Y.; Cohen, I. R.; Fuks, Z.; Vlodavsky, I. Activated T lymphocytes produce a matrix-degrading heparan sulphate endoglycosidase. Nature 310, 1984, 241 - 244. Nishie, A.; Ono, M.; Shono, T.; Fukushi, J.; Otsubo, M.; Onoue, H.; Ito, Y.; Inamura, T.; Ikezaki, K.; Fukui, M. Macrophage infiltration and heme oxygenase-1 expression correlate with angiogenesis in human gliomas. Clin. Cancer Res. 1999, 5, 1107-1113. Okawa, T.; Naomoto, Y.; Nobuhisa, T.; Takaoka, M.; Motoki, T.; Shirakawa, Y.; Yamatsuji, T.; Inoue, H.; Ouchida, M.; Gunduz, M. Heparanase is involved in angiogenesis in esophageal cancer through induction of cyclooxygenase-2. Clin. Cancer Res. 2005, 11, 7995-8005. Pamukcu, A. M.; Yalciner, Ş.; Hatcher, J. F.; Bryan, G. T. Quercetin, a rat intestinal and bladder carcinogen present in bracken fern (Pteridium aquilinum). Cancer Res. 1980, 40, 3468-3472. Parish, C. R.; Freeman, C.; Hulett, M. D. Heparanase: a key enzyme involved in cell invasion. Biochimica et biophysica acta 2001, 1471, 99-108. Park, S. Y.; Park, O. J. Modulation of cancer cell proliferation by cell survival signal Akt and tumor suppressive energy sensor AMP-activated protein kinase in colon cancer cells treated with resveratrol. Food Sci. Biotechnol. 2010, 19, 1537-1541. Patel, B.; Gupta, D.; Sengupta, V.; Majumdar, A. In Mechanisms of growth inhibition of chemo-surviving colon cancer cells by curcumin and resveratrol. Clin. Oncol. 2008, 26, 15088. Pollard, J. W. Tumour-educated macrophages promote tumour progression and metastasis. Nat. Rev. Cancer 2004, 4, 71-78. Powell, S. M.; Zilz, N.; Beazer-Barclay, Y.; Bryan, T. M.; Hamilton, S. R.; Thibodeau, S. N.; Vogelstein, B.; Kinzler, K. W. APC mutations occur early during colorectal tumorigenesis. Nature 1992, 359, 235-237. Pozo‐Guisado, E.; Merino, J. M.; Mulero‐Navarro, S.; Lorenzo‐Benayas, M. J.; Centeno, F.; Alvarez‐Barrientos, A.; Salguero, P. M. F. Resveratrol‐induced apoptosis in MCF‐7 human breast cancer cell sinvolves a caspase‐independent mechanism with downregulation of Bcl‐2 and NF‐κB. Int. J. Cancer 2005, 115, 74-84. R., D. Therapeutic effects of guggul and its constituent guggulsterone: cardiovascular benefits. Cardiovasc. Res. 2007, 25, 375-390. Ranelletti, F. O.; Ricci, R.; Larocca, L. M.; Maggiano, N.; Capelli, A.; Scambia, G.; Benedetti‐Panici, P.; Mancuso, S.; Rumi, C.; Piantelli, M. Growth‐inhibitory effect of quercetin and presence of type‐II estrogen‐binding sites in human colon‐cancer cell lines and primary colorectal tumors. Int. J. Cancer 1992, 50, 486-492. Redente, E. F.; Dwyer-Nield, L. D.; Merrick, D. T.; Raina, K.; Agarwal, R.; Pao, W.; Rice, P. L.; Shroyer, K. R.; Malkinson, A. M. Tumor progression stage and anatomical site regulate tumor-associated macrophage and bone marrow-derived monocyte polarization. J. Pathol. 2010, 176, 2972-2985. Reuter, S.; Eifes, S.; Dicato, M.; Aggarwal, B. B.; Diederich, M. Modulation of anti-apoptotic and survival pathways by curcumin as a strategy to induce apoptosis in cancer cells. Biochem. Pharmacol. 2008, 76, 1340-1351. Samuel, T.; Fadlalla, K.; Turner, T.; Yehualaeshet, T. E. The flavonoid quercetin transiently inhibits the activity of taxol and nocodazole through interference with the cell cycle. Nutrition and cancer 2010, 62, 1025-1035. Santel, T.; Pflug, G.; Hemdan, N. Y. A.; Schafer, A.; Hollenbach, M.; Buchold, M.; Hintersdorf, A.; Lindner, I.; Otto, A.; Bigl, M. Curcumin Inhibits Glyoxalase 1—A Possible Link to Its Anti-Inflammatory and Anti-Tumor Activity. PLoS One 2008, 3, e3508. Schagger, H.; Von Jagow, G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 1987, 166, 368-379. Schoppmann, S. F.; Birner, P.; Stockl, J.; Kalt, R.; Ullrich, R.; Caucig, C.; Kriehuber, E.; Nagy, K.; Alitalo, K.; Kerjaschki, D. Tumor-associated macrophages express lymphatic endothelial growth factors and are related to peritumoral lymphangiogenesis. J. Pathol. 2002, 161, 947-956. Schwende, H.; Fitzke, E.; Ambs, P.; Dieter, P. Differences in the state of differentiation of THP-1 cells induced by phorbol ester and 1, 25-dihydroxyvitamin D3. J. Leukoc. Biol. 1996, 59, 555-561. Seeram, N. P.; Adams, L. S.; Henning, S. M.; Niu, Y.; Zhang, Y.; Nair, M. G.; Heber, D. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J. Nutr. Biochem. 2005, 16, 360-367. Seeram, N. P.; Adams, L. S.; Zhang, Y.; Lee, R.; Sand, D.; Scheuller, H. S.; Heber, D. Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro. J. Agric. Food Chem. 2006, 54, 9329-9339. Sexton, E.; Van Themsche, C.; Leblanc, K.; Parent, S.; Lemoine, P.; Asselin, E. Resveratrol interferes with AKT activity and triggers apoptosis in human uterine cancer cells. Molecular cancer 2006, 5, 45. She, Q. B.; Bode, A. M.; Ma, W. Y.; Chen, N. Y.; Dong, Z. Resveratrol-induced activation of p53 and apoptosis is mediated by extracellular-signal-regulated protein kinases and p38 kinase. Cancer Res. 2001, 61, 1604-1610. Shimada, Y.; Yoshino, M.; Wakui, A.; Nakao, I.; Futatsuki, K.; Sakata, Y.; Kambe, M.; Taguchi, T.; Ogawa, N. Phase II study of CPT-11, a new camptothecin derivative, in metastatic colorectal cancer. CPT-11 Gastrointestinal Cancer Study Group. J. Clin. Oncol. 1993, 11, 909-913. Shishodia, S.; Aggarwal, B. B. Guggulsterone inhibits NF-κB and IκBα kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis. J. Biol. Chem. 2004, 279, 47148-47158. Shteper, P. J.; Zcharia, E.; Ashhab, Y.; Peretz, T.; Vlodavsky, I.; Ben-Yehuda, D. Role of promoter methylation in regulation of the mammalian heparanase gene. Oncogene 2003, 22, 7737-7749. Sica, A.; Schioppa, T.; Mantovani, A.; Allavena, P. Tumour-associated macrophages are a distinct M2 polarised population promoting tumour progression: potential targets of anti-cancer therapy. Eur. J. Cancer 2006, 42, 717-727. Society, A. C., Cancer facts & figures. The Society: 2008. Spencer, J. P. E.; Rice-Evans, C.; Williams, R. J. Modulation of pro-survival Akt/protein kinase B and ERK1/2 signaling cascades by quercetin and its in vivo metabolites underlie their action on neuronal viability. J. Biol. Chem. 2003, 278, 34783-34793. Strimpakos, A. S.; Sharma, R. A. Curcumin: preventive and therapeutic properties in laboratory studies and clinical trials. Antioxid. Redox Signal. 2008, 10, 511-546. Su, L. K.; Kinzler, K. W.; Vogelstein, B.; Preisinger, A. C.; Moser, A. R.; Luongo, C.; Gould, K. A.; Dove, W. F. Multiple intestinal neoplasia caused by a mutation in the murine homolog of the APC gene. Science 1992, 256, 668-670. SUN, C.; Hu, Y.; Guo, T.; WANG, H.; ZHANG, X.; HE, W.; Tan, H. Resveratrol as a novel agent for treatment of multiple myeloma with matrix metalloproteinase inhibitory activity. Acta Pharmacologica Sinica 2006, 27, 1447-1452. Takahashi, H.; Ebihara, S.; Okazaki, T.; Suzuki, S.; Asada, M.; Kubo, H.; Sasaki, H. Clinical significance of heparanase activity in primary resected non-small cell lung cancer. Lung cancer 2004, 45, 207-214. Tan, X.; Hu, D.; Li, S.; Han, Y.; Zhang, Y.; Zhou, D. Differences of four catechins in cell cycle arrest and induction of apoptosis in LoVo cells. Cancer Lett. 2000, 158, 1-6. Thimmulappa, R.; Rangasamy, T.; Alam, J.; Biswal, S. Dibenzoylmethane activates Nrf2-dependent detoxification pathway and inhibits benzo (a) pyrene induced DNA adducts in lungs. Med. Chem. 2008, 4, 473-481. Vattem, D.; Shetty, K. Biological functionality of ellagic acid: a review. J. Food Biochem. 2005, 29, 234-266. Vijayababu, M.; Arunkumar, A.; Kanagaraj, P.; Venkataraman, P.; Krishnamoorthy, G.; Arunakaran, J. Quercetin downregulates matrix metalloproteinases 2 and 9 proteins expression in prostate cancer cells (PC-3). Mol. Cell. Biochem. 2006, 287, 109-116. Vlodavsky, I.; Friedmann, Y.; Elkin, M.; Aingorn, H.; Atzmon, R.; Ishai-Michaeli, R.; Bitan, M.; Pappo, O.; Peretz, T.; Michal, I. Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis. Nat. Med. 1999, 5, 793. Vlodavsky, I.; Friedmann, Y. Molecular properties and involvement of heparanase in cancer metastasis and angiogenesis. J. Clin. Invest. 2001, 108, 341-348. Vlodavsky, I.; Goldshmidt, O.; Zcharia, E.; Atzmon, R.; Rangini-Guatta, Z.; Elkin, M.; Peretz, T.; Friedmann, Y. In Mammalian heparanase: involvement in cancer metastasis, angiogenesis and normal development. Semin. Cancer Biol. 2002, 12, 121-129. Walker, E. H.; Pacold, M. E.; Perisic, O.; Stephens, L.; Hawkins, P. T.; Wymann, M. P.; Williams, R. L. Structural determinants of phosphoinositide 3-kinase inhibition by wortmannin, LY294002, quercetin, myricetin, and staurosporine. Molecular cell 2000, 6, 909-919. Wallace, B. D.; Wang, H.; Lane, K. T.; Scott, J. E.; Orans, J.; Koo, J. S.; Venkatesh, M.; Jobin, C.; Yeh, L. A.; Mani, S. Alleviating cancer drug toxicity by inhibiting a bacterial enzyme. Science 2010, 330, 831-835. Zanotto-Filho, A.; Braganhol, E.; Edelweiss, M. I.; Behr, G. A.; Zanin, R.; Schroder, R.; Simoes-Pires, A.; Battastini, A. M. O.; Moreira, J. C. F. The curry spice curcumin selectively inhibits cancer cells growth in vitro and in preclinical model of glioblastoma. J. Nutr. Biochem. 2011, 23, 591-601 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63578 | - |
| dc.description.abstract | 肝素酶(Heparanase)為一種葡萄醣醛酸內切酶(endo-beta-D-glucuronidase)，具有切割基底細胞膜與組織硫酸肝素鍵結的能力，可以在原位瘤發生惡化時促進腫瘤細胞轉移，且造成腫瘤細胞周遭微環境變化，對於癌症發生的進程有極大的影響。本研究即利用體外細胞模式，以共培養技術，模擬癌症細胞遭受腫瘤微環境因子影響而發生癌症轉移的現象，並以此平台探討植化素(phytochemicals)之介入對目標蛋白(Heparanase)及下游蛋白Akt, Src, MMPs之作用。本研究第一部分，探討以不同條件PMA誘導THP1細胞分化為polarized M2巨噬細胞的效果，發現以320 nM PMA處理 24小時可表現6.6%的polarized M2巨噬細胞表面抗原，並將此細胞在不同時間點(15-240分鐘)誘導HCT116大腸癌細胞株，發現在誘導時間內皆可促使HCT116細胞發生轉移性，其中又以30分鐘及120分鐘共培養效果最佳。第二部分介入文獻中提出具有抑制癌細胞轉移功效的植化素，在測試其對HCT116細胞毒性及酵素活性之影響後，篩選出五種具有潛力之植化素，進入第三部分的西方點墨法分析。結果顯示，以Quercetin及Resveratrol兩者具有最好的抑制轉移效果。在肝素酶蛋白表現上，以後介入方式施予Quercetin，具有抑制HPA酵素蛋白表現的功效，且有濃度效應，而在p-Src與p-Akt方面，以前介入或後介入方式皆有抑制的效果，而Resveratrol在Heparanase蛋白表現上並無抑制作用，但其可影響Heparanase下游蛋白Akt與Src，使其磷酸化降低，而達到抑制轉移的現象。總括而言，Quercetin為七種植化素中擁有最佳抑制轉移的能力，可以在細胞模式中經由抑制誘發的HCT116肝素酶表現，達到抑制癌症轉移的效果。 | zh_TW |
| dc.description.abstract | Heparanase is a type of endo-beta-D-glucuronidase which can cleave heparan sulfate (HS) chains both at the cell surface and in the extracellular matrix. The enzyme is secreted by metastatic tumor cells, destroys surrounding tissue, and alters the microenviroment of the cells. In this study, we used an in vitro cell model which is established by co-cultivation of cancer cells and the tumor microenvironment factors. Then, we investigated the effect of some phytochemicals on the metastasis of the cancer cells. The first part of this study used different concentration of PMA to induce differentiation of THP1 cells to polarized M2 macrophage, and found that 320 nM PMA increase 6.6% polarized M2 cell’s surface antibody. After that, we used the polarized M2 macrophage to induce HCT116 cell at different time points to establish the metastasis mode. We found that the induction occurred at 30 minute and 120 minute resulted in the highest degree of metastasis. In the second part of this study, we first investigated the effects of the some potential phytochemicals on the cell survival rate and | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:14:16Z (GMT). No. of bitstreams: 1 ntu-100-R99641023-1.pdf: 5082399 bytes, checksum: b24de1e36f017e7e7178c0001ea4aad4 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 謝誌 I
中文摘要 IV 圖次 IX 表次 XI 壹、緒論 1 一、大腸直腸癌 1 (一)人類大腸直腸癌(colorectal cancer)之特性 1 (二)大腸直腸癌治療方式 4 (三)大腸直腸癌致癌機轉研究 7 (四)腫瘤微環境與癌症發展之關係 10 二、肝素酶 10 (一)肝素酶(Heparanase)簡介 10 (二)肝素酶表現於臨床病例增加癌症轉移 11 (三)肝素酶於細胞試驗增進血管新生與侵襲、轉移 12 (四)肝素酶與大腸直腸癌的關係 12 三、腫瘤相關巨噬細胞 13 (一)腫瘤相關巨噬細胞(tumor-associated macrophage, TAM)簡介 13 (二)腫瘤相關巨噬細胞促進腫瘤細胞侵襲與轉移 15 (三)腫瘤相關巨噬細胞增進腫瘤細胞血管新生 16 (四)腫瘤相關巨噬細胞與大腸直腸癌轉移關係 17 (五)以PMA誘導單核球細胞THP1分化成腫瘤相關巨噬細胞 17 四、植化素(phytochemicals) 19 (一)植化素簡介 19 (二)植化素的生理機能活性 19 貳、研究目的與實驗架構 23 一、研究目的 23 二、實驗架構 24 第一部分、建立評估轉移試驗的細胞模式 24 第二部分、篩選植物化學物質 25 第三部分、肝素酶於大腸癌細胞誘導轉移的影響與作用原理 26 参、材料與方法 27 一、實驗材料 27 (一)細胞株來源 27 (二)藥品試劑 27 (三)儀器設備 29 二、實驗方法 31 (一) 細胞培養(cell culture) 31 (二) 樣品配製 32 (三) 細胞存活率(MTT assay) 32 (四) 酵素活性試驗(b-D-glucurosidase active assay) 34 (五) PMA (Phorbol-12-myristate-13-acetate)誘導單核球細胞分化為巨噬細胞 ……………………………………………………………………………………………………………..36 (六) 流式細胞儀檢測巨噬細胞表面標記 37 (七) 巨噬細胞與大腸癌細胞共培養 38 (八) 轉移試驗(Migration assay) 40 (九) 蛋白質萃取與定量 42 (十) SDS-PAGE電泳分析 43 (十一)西方點墨法(Western blotting) 45 (十二) 酵素免疫分析(Enzyme-linked immunosorbent assay, ELISA) 46 (十三) 統計分析(Statistics analysis) 47 肆、結果與討論 48 一、單核球細胞株THP1經不同PMA濃度誘導產生的型態變化 48 二、單核球細胞株THP1經不同PMA濃度誘導分化為M2 macrophage (Tumor associated macrophage, TAM) 50 三、大腸癌細胞HCT116與320 nM PMA誘導M2細胞共培養 52 四、植化素對人類大腸直腸癌細胞株HCT116存活率之影響 55 五、以Migration assay 初步檢測各植化素在此模式中的抑制效果 58 六、利用西方點墨法探討經M2 Macrophage誘導之人類大腸癌細胞株HCT116肝素酶之表現 64 七、利用西方墨點法探討經M2 Macrophage誘導之 HCT116細胞之 Heparanase相關蛋白質之表現 66 八、Quercetin對M2巨噬細胞誘導轉移之HCT116細胞之HPA相關蛋白質表現之影響 71 (一) Heparanase(HPA)蛋白表現量 73 (二) Src與p-Src蛋白表現量 75 (三) Akt與p-Akt蛋白表現量 77 (四) MMP-2與MMP-9蛋白表現量 78 九、Resveratrol對M2巨噬細胞誘導轉移之HCT116細胞之 HPA相關蛋白質表現之影響 81 (一) Heparanase (HPA)蛋白表現量 81 (二) Src與p-Src蛋白表現量 83 (三) Akt與p-Akt蛋白表現量 85 (四) MMP-2與MMP-9蛋白表現量 85 伍、結論與展望 90 陸、參考文獻 91 附錄期刊格式 105 | |
| dc.language.iso | zh-TW | |
| dc.subject | HCT116 | zh_TW |
| dc.subject | 腫瘤相關的巨噬細胞 | zh_TW |
| dc.subject | 肝素酶 | zh_TW |
| dc.subject | 癌症轉移 | zh_TW |
| dc.subject | 腫瘤微環境 | zh_TW |
| dc.subject | heparanase | en |
| dc.subject | Tumor associated macrophage | en |
| dc.subject | metastasis cancer | en |
| dc.title | 植化素經由抑制肝素酶活性對預防大腸直腸癌轉移之功效 | zh_TW |
| dc.title | Anti-metastasis effect of phytochemicals on colorectal cancer cells via heparanase inhibitory activity | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鐘景光(Jing-Gung Chung),何其儻(Chi-Tang Ho),吳明賢(Ming-Shiang Wu) | |
| dc.subject.keyword | 肝素酶,腫瘤相關的巨噬細胞,癌症轉移,HCT116,腫瘤微環境, | zh_TW |
| dc.subject.keyword | heparanase,Tumor associated macrophage,metastasis cancer, | en |
| dc.relation.page | 113 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-20 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 食品科技研究所 | zh_TW |
| 顯示於系所單位： | 食品科技研究所 | |
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