水稻 stunt lemma palea 1 突變對偶基因的功能探討

Abstract

Stunted lemma/palea (slp) 為一水稻突變品系，其外表型呈現矮株、內外穎退化與不稔。前人研究的結果顯示slp突變外表型可能是由於OsSPL16蛋白序列中第六個胺基酸發生錯義突變而造成。本研究顯示過量表現正常OsSPL16對偶基因於slp突變植株中並無法使slp突變外表型回復正常，然而將突變OsSPL16對偶基因導入正常水稻植株中會造成轉殖植株呈現slp突變外表型。此結果證實OsSPL16的錯義突變對偶基因確實為slp突變對偶基因，而此突變對偶基因也具有活性，能影響水稻株高及花穗發育。

The rice stunted lemma/palea (slp) mutant displays dwarf, shorten panicle length, seriously degenerated lemma/palea, and sterility. Previous study suggested that a missense mutation at the sixth amino acid of the OsSPL16 protein was likely responsible for the slp mutant phenotypes. The current study showed that overexpression of the wild-type OsSPL16 allele in the slp/slp and Slp/slp mutants was unable to convert the slp mutant phenotype to normal. However, introduction of the mutant OsSPL16 allele into a normal rice cultivar resulted in the slp mutant phenotypes displayed in the transgenic plants. These results concluded the missense mutation of OsSPL16 is the slp mutant allele and a neomorph allele which affects plant height and development of inflorescence and spikelet.