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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳凱儀(Kai-Yi Chen) | |
| dc.contributor.author | Ya-Ling Hou | en |
| dc.contributor.author | 侯雅玲 | zh_TW |
| dc.date.accessioned | 2021-06-16T16:12:06Z | - |
| dc.date.available | 2018-04-26 | |
| dc.date.copyright | 2013-04-26 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-02-18 | |
| dc.identifier.citation | 康家豪 (2007) 以DNA微陣列分析slpslp突變水稻之幼花序基因表現。台灣大學農藝系碩士論文.
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(2009) RETARDED PALEA1 controls palea development and floral zygomorphy in rice. Plant Physiol 149:235-244. Zhang Q, Xu J, Li Y, Xu P, Zhang H, Wu X. (2007) Morphological, anatomical and genetic analysis for a rice mutant with abnormal hull. J Genet Genomics. 34:519-26. Zhang Y, Schwarz S, Saedler H, Huijser P (2007) SPL8, a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis. Plant Mol. Biol. 63: 429-439. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62841 | - |
| dc.description.abstract | Stunted lemma/palea (slp) 為一水稻突變品系，其外表型呈現矮株、內外穎退化與不稔。前人研究的結果顯示slp突變外表型可能是由於OsSPL16蛋白序列中第六個胺基酸發生錯義突變而造成。本研究顯示過量表現正常OsSPL16對偶基因於slp突變植株中並無法使slp突變外表型回復正常，然而將突變OsSPL16對偶基因導入正常水稻植株中會造成轉殖植株呈現slp突變外表型。此結果證實OsSPL16的錯義突變對偶基因確實為slp突變對偶基因，而此突變對偶基因也具有活性，能影響水稻株高及花穗發育。 | zh_TW |
| dc.description.abstract | The rice stunted lemma/palea (slp) mutant displays dwarf, shorten panicle length, seriously degenerated lemma/palea, and sterility. Previous study suggested that a missense mutation at the sixth amino acid of the OsSPL16 protein was likely responsible for the slp mutant phenotypes. The current study showed that overexpression of the wild-type OsSPL16 allele in the slp/slp and Slp/slp mutants was unable to convert the slp mutant phenotype to normal. However, introduction of the mutant OsSPL16 allele into a normal rice cultivar resulted in the slp mutant phenotypes displayed in the transgenic plants. These results concluded the missense mutation of OsSPL16 is the slp mutant allele and a neomorph allele which affects plant height and development of inflorescence and spikelet. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T16:12:06Z (GMT). No. of bitstreams: 1 ntu-102-R98621111-1.pdf: 3510283 bytes, checksum: c7fa21aadb66a7d667b2841f19b1b823 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
致謝 ii 中文摘要 iii Abstract iv 目錄 v 圖目錄 vi 表目錄 vi Introduction 1 Materials and methods 4 Genomic DNA extraction 4 Genotying 4 RNA extraction 4 cDNA synthesis 5 RT-PCR analysis 6 Plasmid construction and plant transformation 6 Pyro-sequencing for quantitative analysis of copy number of transgenic mutant alleles 7 GUS staining 8 Result 9 Phenotype characterization of the NG5065 mutant 9 OsSPL16 expression pattern 9 Phenotype characterization of PcDD, OxH and OxM transgenic plants 10 Phenotype characterization of PmD transgenic plants 11 Discussion 13 The slp mutation is a neomorph 13 The SNP mutation of slp involves in glume development 13 Candidate genes regulated by OsSPL16 14 Reference 16 圖目錄 Figure 1. Schematic diagram of NG5065- Tos17 insertion mutant. 21 Figure 2. The grain morphology of the NG5065 line. 22 Figure 3. Expression activity of pSPL16::GUS in different tissues and organs. 23 Figure 4. The plant and grain morphology of OxH transgenic plants. 24 Figure 5. The morphology of T0 OxM transgenic plants. 25 Figure 6. Panicles of the OxM transgenic plants. 26 Figure 7. RT-PCR analysis on introduced OsSPL16 gene in OxH and OxM lines. 27 Figure 8. The panicle structure and fertile grain in PmD transgenic rice plants. 29 Figure 9. Grain morphology in PmD transgenic plants. 30 表目錄 Table 1. The phenotypes and genotypes of the T0 PmD transgenic lines. 31 附錄目錄 Appendix 1. Sequence comparison of the OsSPL16 gene between the Slp/Slp and slp/slp genotypes. 33 Appendix 2. Primer pairs used for the RT-PCR analysis 34 Appendix 3. The pSPL16com plasmid and construct flow chart. 35 Appendix 4. The pSPL16OxC plasmid and construct flow chart. 36 Appendix 5. pSPL16m plasmid and construct flow chart. 37 Appendix 6. pSPL16GUS plasmid and construct flow chart. 38 Appendix 7. Primer pairs list 39 Appendix 8. Gene expression difference up to 5x between Slp/Slp and slp/slp young inflorescence were listed. 40 | |
| dc.language.iso | en | |
| dc.subject | 內外穎；水稻；突變 | zh_TW |
| dc.subject | glumes；rice；mutant | en |
| dc.title | 水稻 stunt lemma palea 1 突變對偶基因的功能探討 | zh_TW |
| dc.title | Functional characterization of the stunt lemma palea 1 mutant allele in rice | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳良築(Liang-Jwu Chen),常玉強(Yuh-Chyang Charng),洪傳揚(Hong Chwan-Yang) | |
| dc.subject.keyword | 內外穎；水稻；突變, | zh_TW |
| dc.subject.keyword | glumes；rice；mutant, | en |
| dc.relation.page | 41 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-02-18 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
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