冷休克在斑馬魚所引發之微型核醣核酸和轉錄體圖譜變化

Abstract

Cold shock is an acute reduction in ambient temperature that may cause rapid falling in body temperature and a cascade of physiological and behavioral responses. It is a big challenge for the survival of fish during winter. Previous works have provided solid information for fish mortality and physiological responses associated with cold shock. However, little is known for the molecular mechanisms regulating cold-shock responses. MicroRNAs (miRNAs) are noncoding small RNAs which play essential regulatory roles in development, cell differentiation, and metabolism. Moreover, few studies studied the roles of miRNAs for physiological response to environmental stresses in fish. To explore the role of miRNAs during cold shock, we performed genome-wide zebrafish miRNAs pattern analysis. We found 29 up-regulated miRNAs and 26 down-regulated miRNAs in cold-treated zebrafish larvae and obtained their target genes by bioinformatics analysis. We also found 1,424 cold-dependent genes using transcriptomic analysis. These genes are significant involved in signaling pathway, ligand-receptor interaction, lipid metabolism and biosynthesis. These genes are functional relevant to cold shock stress. In conclusion, our results reveal a potential regulatory role of miRNAs in underline cellular responses induced by cold shock stress. Here, we have studied several of those miRNAs and their target gene, such as circadian genes, to explore their roles in cold shock regulation.