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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 游若? | |
dc.contributor.author | Lang-Wei Lai | en |
dc.contributor.author | 賴俍瑋 | zh_TW |
dc.date.accessioned | 2021-06-16T13:21:37Z | - |
dc.date.available | 2013-11-05 | |
dc.date.copyright | 2013-11-05 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-07-25 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61981 | - |
dc.description.abstract | Saccharomyces cerevisiae 富含維生素B群及多種營養素過去常被用來做為營養補充劑,也曾被用來做為益生菌及益生質等功能性食品。結腸直腸癌在台灣是主要的死亡原因之一,大約有70% 的結腸直腸癌成因與飲食有關。研究指出益生菌可以和致突變物作用,降低致突變物質對腸道細胞造成的傷害。因此本試驗研究5株S. cerevisiae細胞區分物 (熱致死細胞、粗細胞壁及胞內液) 對人類結腸癌細胞株HT-29增生及致突變劑4-nitroquinoline-N-oxide (4NQO) 對正常腸道細胞 Int-407 細胞毒性之效果,進一步利用分段預反應探討其抗致突變的機制,並利用彗星電泳 (Comet assay) 檢測區分物抑制4NQO誘導Int-407基因毒性之效果。結果顯示熱致死細胞及粗細胞壁具有抑制大腸癌細胞HT-29增生的效果,其中以粗細胞壁為佳,抑制率為 24.5% -52.83%。在抗細胞毒性方面,熱致死細胞及粗細胞壁同樣具有減低4NQO對於正常腸道細胞的毒性,以熱致死細胞效果較好,可使細胞存活率由 49.7%-54.6% 提升至 73.3%-82.2%。在抑制基因毒性方面,熱致死細胞及粗細胞壁也能降低4NQO誘導Int-407產生的基因損傷,損傷分數由 134.0-146.7 降至 79.0-90.0 及 76.0- 93.3。利用分段預反應探討其抗致突變的機制,發現熱致死細胞及粗細胞壁皆具有阻斷效應及生物抗細胞毒性,能調整細胞機能來抵抗 4NQO 的致突變作用,並幫助細胞修復損傷。而熱致死細胞比粗細胞壁多了去細胞毒性的功效,能直接和致突變劑 4NQO 作用,降低其毒性。 | zh_TW |
dc.description.abstract | Saccharomyces cerevisiae is not only an excellent source of B-complex vitamins but also a source of functional food as probiotics or prebiotics. Colorectal cancer is major cause of death in Taiwan. Approximately 70% of colorectal cancer is related to diets. Probiotics have positive health effects such as antioxidative, antitumor and antimutagenic activity. The objective of this study was to investigate effects of several S. cerevisiae cell fractions on the proliferation of human colon cancer cell, HT29, and the prevention of 4NQO-induced DNA damage on Int-407 cells. The possible anticytotoxic mechanisms were investigated by section-preincubation test. Comet assay explored the effects of yeast cell fraction against 4NQO-induced genetoxicity. The result revealed that heat-killed cells and crude cell walls exhibited the antiproliferation activity on HT-29 cells.The antiproliferation rate was 24.5%-52.83%. It was noted that heat-killed cells and crude cell walls reduced the cytotoxicity of 4-NQO against Int-407 showing the anticytotoxic activity. The viability of Int-407 increased from 49.7%-54.6% to 73.3%-82.2%. Heat-killed cells and crude cell walls also significantly reduced the 4NQO-induced genetoxicity. The scores of DNA damage decreased from 134.0-146.7 to 79.0-90.0 and 76.0- 93.3. It was found that heat-killed cells and crude cell walls might exert blocking and bioanticytotoxic effects by both adjusting the function of intestine 407 and repairing the 4-NQO-damaged cells. Additionally, heat-killed cells might exert descytotoxic effects by bioremediation, thus reducing cytotoxicity of 4-NQO. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T13:21:37Z (GMT). No. of bitstreams: 1 ntu-102-R00641019-1.pdf: 2132079 bytes, checksum: 4aec9e505d6537f4d8302cc0390b9ee3 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 壹、前言 1
貳、文獻整理 2 一、Saccharomyces cerevisiae 2 (一)、釀酒用酵母菌 2 (二)、S. cerevisiae 3 (三)、S. cerevisiae 細胞壁組成 3 (四)、S. cerevisiae 相關功能性研究 6 二、益生菌(probiotics) 6 三、結腸直腸癌 13 四、致突變物與抗致突變物 16 (一)、飲食與癌症 16 (二)、食品中存在的致突變物 16 (三)、飲食中之抗致突變劑 19 (四)、抗致突變之機制 20 五、致突變劑 4-Nitrowuinoline-N-oxide (4NQO) 23 參、材料與方法 25 一、 實驗架構 25 二、 實驗材料 27 (一)、試驗菌株 27 (二)、試驗細胞株 27 (三)、培養基 27 (四)、藥品 28 (五)、器材設備 29 三、實驗方法 30 (一)、酵母菌的保存與活化 30 (二)、酵母菌生長曲線之測定 30 (三)、酵母菌區分物之製備 30 (四)、人類結腸癌 HT29細胞株與人類正常腸道上皮細胞 Int-407細胞株之培養與保存 32 (五)、MTT 細胞存活率試驗 34 (六)、S. cerevisiae細胞區分物抑制人類結腸癌細胞株HT-29 增生試驗 34 (七)、S. cerevisiae細胞區分物對於人類腸道細胞Int-407 存活率之影響 35 (八)、S. cerevisiae細胞區分物抑制4NQO 對Int-407 之細胞毒性試驗 35 (九)、彗星電泳試驗(Comet assay) 36 (十)、S. cerevisiae細胞區分物中熱致死細胞及粗細胞壁抑制4NQO 對Int-407 之細胞毒性機制之探討 38 (十一)、 統計分析 39 肆、結果與討論 40 一、S. cerevisiae細胞區分物對人類結腸癌細胞株 HT-29 細胞存活率之影響 40 二、S. cerevisiae細胞區分物對人類腸道上皮細胞 Int-407 存活率之影響 42 三、S. cerevisiae細胞區分物抑制 4NQO 誘導人類腸道上皮細胞 Int-407 細胞毒性 44 四、 S. cerevisiae細胞區分物抑制 4NQO 誘導之基因毒性 46 五、 S. cerevisiae細胞區分物中熱致死細胞及粗細胞壁抑制 4NQO 誘導細胞毒性機制之探討 52 (一)、 S. cerevisiae細胞區分物中熱致死細胞及粗細胞壁的去細胞毒性效應 (Descytotoxic effects) 52 (二)、S. cerevisiae細胞區分物中熱致死細胞及粗細胞壁的阻擋效應 (Blocking effects) 54 (三)、S. cerevisiae細胞區分物中熱致死細胞及粗細胞壁的生物抗細胞毒性效應 (Bioanticytotoxic effects) 56 伍、結論 59 陸、參考文獻 61 柒、附錄 71 | |
dc.language.iso | zh-TW | |
dc.title | 數株 Saccharomyces cerevisiae 及其區分物抑制大腸癌細胞增生及大腸細胞株抗細胞毒性研究 | zh_TW |
dc.title | Antiproliferation of colon cancer cell line HT-29 and anticytotoxicity of human intestine cell line Int-407 by the cell fractions of several Saccharomyces cerevisiae strains | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 周正俊,丘志威,蔡國珍,潘崇良 | |
dc.subject.keyword | Saccharomyces cerevisiae,大腸直腸癌,抗細胞毒性,彗星電泳, | zh_TW |
dc.subject.keyword | Saccharomyces cerevisiae,colon cancer,anticytotoxic,comet assay, | en |
dc.relation.page | 90 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-07-25 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
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