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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6085
Title: | 使用RAD定序方法進行臺稉14號與桃園1號雜交組合之耐穗上發芽數量性狀基因座的遺傳定位 QTL Mapping for Pre-harvest Sprouting in the Cross between TK14 and TY1 Varieties Using RAD Sequencing |
Authors: | Ming-Hsiu Hsieh 謝明修 |
Advisor: | 陳凱儀 |
Keyword: | 水稻,穗上發芽,休眠性,數量性狀基因座定位, Oryza sativa,pre-harvest sprouting,seed dormancy,QTL mapping,selective genotyping, |
Publication Year : | 2013 |
Degree: | 碩士 |
Abstract: | 水稻穗上發芽 (pre-harvest sprouting) 是指生理成熟的穎果在收穫前發芽,而造成產量損耗與稻米品質下降。我們選用高穗上發芽率品種臺稉 14 號以及低穗上發芽率品種桃園 1 號,建立 F2 遺傳定位族群並進行穗上發芽與種子休眠之數量性狀基因座分析。我們在 F2 族群中挑選出穗上發芽率最高與最低的個體各 30 株,並萃取這些植株的 DNA 來進行 RAD (Restriction-site Associated DNA) 定序,以便同時開發高密度的 SNP 分子標記並完成選拔個體之所有分子標記的基因型鑑定。我們亦由此 60 個 F2 個體繁殖成 F2:3 家系,進一步觀察穗上發芽與種子休眠維持日數之關聯性。試驗結果顯示穗上發芽與種子休眠性之數量性狀基因座被定位在第 3 對染色上相同的染色體區間。此外,高休眠性之遺傳重組家系皆具有低穗上發芽的特性,但在低休眠性之遺傳重組家系中則呈現高度差異的穗上發芽率。 Preharvest sprouting in rice is germination of physiologically ripe kernel before harvest, which leads yield loss and bad grain quality. We made an F2 genetic mapping population by the cross of TK14, a variety with high preharvest sprouting rate, and TY1, a variety with low preharvest sprouting rate, and then conducted QTL analysis for preharvest sprouting and seed dormancy. We selected 30 individuals with each of highest and lowest preharvest sprouting rate in the F2 population, and extracted their DNA to do RAD (Restriction-site Associated DNA) sequencing, in order to develop high-density SNP markers and to complete all marker genotypes of these selected individuals simultaneously. We also generated F2:3 families from these selected F2 individuals and investigated the relationship between preharvest sprouting and seed dormancy. The results showed that QTLs for both preharvest sprouting and seed dormancy were detected on the same region of chromosome 3. Also, all families with strong seed dormancy showed low preharvest sprouting rate. However, families with weak seed dormancy show highly variable preharvest sprouting rate. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6085 |
Fulltext Rights: | 同意授權(全球公開) |
Appears in Collections: | 農藝學系 |
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File | Size | Format | |
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ntu-102-1.pdf | 2.78 MB | Adobe PDF | View/Open |
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