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標題: | 利用倍頻顯微術對日光引致慢性皮膚傷害之特徵分析 Using Harmonic Generation Biopsy to Characterize Sun-induced Chronic Damage of Skin |
作者: | Yu-Hsiang Su 蘇郁翔 |
指導教授: | 孫啟光(Chi-Kuang Sun) |
關鍵字: | 倍頻顯微術 (HGM),曬斑 (solar lentigo),活體光學虛擬切片,醫療美容,治療效果評估, harmonic generation microscopy,solar lentigo,in vivo optical biospy,aesthetic medicine,therapeutic evaluation, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 日常生活裡暴露在日光內的紫外光輻射下會造成皮膚的慢性傷害與病變,給人帶來很大的顧慮擔憂。為了研究慢性病變異常的進程以及皮膚內的動態變化,本論文使用倍頻顯微虛擬切片作為有利的活體顯影技術來研究皮膚中的病變特徵。此技術能提供即時造影,擁有對細胞病理學診斷非常關鍵的次微米級的解析度,而且在造影過程對生物組織留下的光傷害是極小的。曬斑是一種皮膚內陽光引致的超量色素沉澱,在本論文中以其作為主要的研究課題,利用活體倍頻顯微虛擬切片來定義曬斑中的特徵。包括病灶處本身的特徵,或是經過雷射除斑治療的變化都藉由活體倍頻顯微虛擬切片來觀察。十七位亞裔女性志願者參與了本研究相關的臨床試驗。志願者們的年齡分布從四十一至七十七歲,並滿足在臉上有曬斑且準備接受治療之條件。擷取的活體倍頻顯微虛擬切片影像用來進行分析與特徵之參數量化。六種關於曬斑皮膚內黑色素含量或組織結構的參數被量化分析。
論文中揭示了在曬斑病灶區域中,角質層厚度、表皮真皮交界層附近之基底細胞細胞質內黑色素質量密度 (MMDDEJ)、以及二維軸向縱切細胞尺寸三者皆增加了。在活體影像中定量出病灶處MMDDEJ增加的發現是前所未見的。比起過往研究指出的觀測皮膚中黑色素多寡之定量方法,MMDDEJ應是較為可靠且更方便進行客觀比較的參數。在經過雷射除斑手術之後,二維俯瞰的富含黑色素之細胞分布之面積密度,以及角質層厚度都有減少。且進一步的分析指出富含黑色素之細胞分布之面積密度以及MMDDEJ的減少似乎與外表淡斑的改善效果有正相關。藉由這樣的分析可以進一步推論雷射治療中選擇性色素光破壞帶來的改善效果可能是富含黑色素之細胞的大量去除,以及降低基底細胞中平均含有的黑色素量。 The chronic damage of skin caused by cumulated exposure to ultraviolet radiation has brought great concern. To study the changes in skin dynamics, in vivo harmonic generation biopsy (HGB) was proposed as a potent imaging tool. HGB provides submicron resolution crucial for histopathological examination and leaves little optical damage in biotissues. With the use of HGB, this thesis is aimed at seeking out novel characteristics in solar lentigines, a type of sun-induced hyperpigmentation, both in their developing progress and after laser depigmenting treatments. Seventeen female Asian volunteers aged from 41 to 77 years old with solar lentigines on the face were involved in the study. Six parameters in HGB images concerning melanin contents or skin structure in lentigines were quantified. It is shown that in solar lentigines 2D longitudinal cellular size, the thickness of stratum corneum (SC), and melanin mass density in cytoplasm of basal cells near dermoepidermal junction (MMDDEJ) are increased. The finding of increase of MMDDEJ in vivo is unprecedented. MMDDEJ was believed to be more objectively comparable than the quantification of melanin proposed by previous studies. The significant decreases in 2D en face viewed melanin-rich cell density and thickness of SC after laser treatment were discovered. It was shown that the decreases in MMDDEJ and 2D melanin-rich cell density seem to have a positive correlation with the whitening of appearances on skin. It may be inferred that the main mechanisms which determine the effects from laser treatments are the removal of melanin-rich cells and the decrease of average melanin content in basal cells. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60065 |
DOI: | 10.6342/NTU201603818 |
全文授權: | 有償授權 |
顯示於系所單位: | 光電工程學研究所 |
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