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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 孫啟光(Chi-Kuang Sun) | |
dc.contributor.author | Yu-Hsiang Su | en |
dc.contributor.author | 蘇郁翔 | zh_TW |
dc.date.accessioned | 2021-06-16T09:53:52Z | - |
dc.date.available | 2022-02-08 | |
dc.date.copyright | 2017-02-08 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-01-10 | |
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[25] Richtig E, Hofmann-Wellenhof R, Kopera D, El-Shabrawi-Caelen L, and Ahlgrimm-Siess V, “In vivo analysis of solar lentigines by reflectance confocal microscopy before and after Q-switched Ruby laser treatment,“ Acta Derm Venereol. 2011 Mar;91(2):164-8. [26] Pollefliet C, Corstjens H, Gonzalez S, Hellemans L, Declercq L, and Yarosh D, “Morphological characterisation of Solar Lentigines by in vivo Reflectance Confocal Microscopy: a longitudinal approach,” Int J Cosmet Sci. 2013 Apr;35(2):149-55. [27] Brown Lance H, “Treating Solar Lentigines: Traditional treatments at a glance — plus, a look at a cutting-edge option.” The Dermatologist.com, Volume 10 - Issue 8 - August 2002. 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[32] Vachiramon V, Panmanee W, Techapichetvanich T, and Chanprapaph K, “Comparison of Q-switched Nd: YAG laser and fractional carbon dioxide laser for the treatment of solar lentigines in Asians,” Lasers Surg Med. 2016 Apr;48(4):354-9. [33] Ghaninejhadi H, Ehsani A, Edrisi L, Gholamali F, Akbari Z, and Noormohammadpour P, “Solar Lentigines: Evaluating Pulsed Dye Laser (PDL) as an Effective Treatment Option,” J Lasers Med Sci. 2013 Winter; 4(1): 33-8. [34] Sun CK, Chen CC, Chu SW, Tsai TH, Chen YC, and Lin BL, “Multiharmonic generation biopsy of skin,” Opt Lett. 2003 Dec 15;28(24):2488-90. [35] Chen SY, Wu HY, and Sun CK, “In vivo harmonic generation biopsy of human skin,” J Biomed Opt. 2009 Nov-Dec;14(6):060505. [36] Chen SY, Chen SU, Wu HY, Lee WJ, Liao YH, and Sun CK, “In vivo virtual biopsy of human skin by using noninvasive higher harmonic generation microscopy,” IEEE J. Sel. Topics Quantum Electron. 2010; 16(3): 478-92. [37] Anderson RR and Parish JA, “The optics of human skin,” J Invest Dermatol. 1981 Jul;77(1):13-9. [38] Chen IH, Chu SW, Sun CK, Cheng PC, and Lin BL, “Wavelength dependent damage in biological multi-photon confocal microscopy: A micro-spectroscopic comparison between femtosecond Ti:sapphire and Cr:forsterite laser sources.” Opt. Quantum Electron. 2002; 34:1251. [39] Hsieh CS, Chen SU, Lee YW, Yang YS, and Sun CK, “Higher harmonic generation microscopy of in vitro cultured mammal oocytes and embryos,” Opt Express. 2008 Jul 21;16(15):11574-88. [40] Liu WM, “In Vivo Quantification of Melanin Mass Density by Using Third Harmonic Generation Microscopy,” 2013 [41] Lagarrigue SG, George J, Questel E, Lauze C, Meyer N, Lagarde JM, Simon M, Schmitt AM, Serre G, and Paul C, “In vivo quantification of epidermis pigmentation and dermis papilla density with reflectance confocal microscopy: variations with age and skin phototype” Exp Dermatol. 2012 Apr;21(4):281-6. [42] Otsu N. 'A threshold selection method from gray-level histograms'. IEEE Trans. Sys., Man., Cyber. 1979; 9 (1): 62-6. [43] Lee GG, Lin HH, Tsai MR, Chou SY, Lee WJ, Liao YH, Sun CK, and Chen CF, “Automatic Cell Segmentation and Nuclear-to-Cytoplasmic Ratio Analysis for Third Harmonic Generated Microscopy Medical Images,” IEEE Trans Biomed Circuits Syst. 2013 Apr;7(2):158-68. [44] Matts PJ, Dykes PJ, and Marks R, “The distribution of melanin in skin determined in vivo.” Br J Dermatol. 2007 Apr;156(4):620-8. [45] Crowther JM, Sieg A, Blenkiron P, Marcott C, Matts PJ, Kaczvinsky JR, and Rawlings AV, “Measuring the effects of topical moisturizers on changes in stratum corneum thickness, water gradients and hydration in vivo,” Br J Dermatol. 2008 Sep;159(3):567-77. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60065 | - |
dc.description.abstract | 日常生活裡暴露在日光內的紫外光輻射下會造成皮膚的慢性傷害與病變,給人帶來很大的顧慮擔憂。為了研究慢性病變異常的進程以及皮膚內的動態變化,本論文使用倍頻顯微虛擬切片作為有利的活體顯影技術來研究皮膚中的病變特徵。此技術能提供即時造影,擁有對細胞病理學診斷非常關鍵的次微米級的解析度,而且在造影過程對生物組織留下的光傷害是極小的。曬斑是一種皮膚內陽光引致的超量色素沉澱,在本論文中以其作為主要的研究課題,利用活體倍頻顯微虛擬切片來定義曬斑中的特徵。包括病灶處本身的特徵,或是經過雷射除斑治療的變化都藉由活體倍頻顯微虛擬切片來觀察。十七位亞裔女性志願者參與了本研究相關的臨床試驗。志願者們的年齡分布從四十一至七十七歲,並滿足在臉上有曬斑且準備接受治療之條件。擷取的活體倍頻顯微虛擬切片影像用來進行分析與特徵之參數量化。六種關於曬斑皮膚內黑色素含量或組織結構的參數被量化分析。
論文中揭示了在曬斑病灶區域中,角質層厚度、表皮真皮交界層附近之基底細胞細胞質內黑色素質量密度 (MMDDEJ)、以及二維軸向縱切細胞尺寸三者皆增加了。在活體影像中定量出病灶處MMDDEJ增加的發現是前所未見的。比起過往研究指出的觀測皮膚中黑色素多寡之定量方法,MMDDEJ應是較為可靠且更方便進行客觀比較的參數。在經過雷射除斑手術之後,二維俯瞰的富含黑色素之細胞分布之面積密度,以及角質層厚度都有減少。且進一步的分析指出富含黑色素之細胞分布之面積密度以及MMDDEJ的減少似乎與外表淡斑的改善效果有正相關。藉由這樣的分析可以進一步推論雷射治療中選擇性色素光破壞帶來的改善效果可能是富含黑色素之細胞的大量去除,以及降低基底細胞中平均含有的黑色素量。 | zh_TW |
dc.description.abstract | The chronic damage of skin caused by cumulated exposure to ultraviolet radiation has brought great concern. To study the changes in skin dynamics, in vivo harmonic generation biopsy (HGB) was proposed as a potent imaging tool. HGB provides submicron resolution crucial for histopathological examination and leaves little optical damage in biotissues. With the use of HGB, this thesis is aimed at seeking out novel characteristics in solar lentigines, a type of sun-induced hyperpigmentation, both in their developing progress and after laser depigmenting treatments. Seventeen female Asian volunteers aged from 41 to 77 years old with solar lentigines on the face were involved in the study. Six parameters in HGB images concerning melanin contents or skin structure in lentigines were quantified. It is shown that in solar lentigines 2D longitudinal cellular size, the thickness of stratum corneum (SC), and melanin mass density in cytoplasm of basal cells near dermoepidermal junction (MMDDEJ) are increased. The finding of increase of MMDDEJ in vivo is unprecedented. MMDDEJ was believed to be more objectively comparable than the quantification of melanin proposed by previous studies. The significant decreases in 2D en face viewed melanin-rich cell density and thickness of SC after laser treatment were discovered. It was shown that the decreases in MMDDEJ and 2D melanin-rich cell density seem to have a positive correlation with the whitening of appearances on skin. It may be inferred that the main mechanisms which determine the effects from laser treatments are the removal of melanin-rich cells and the decrease of average melanin content in basal cells. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:53:52Z (GMT). No. of bitstreams: 1 ntu-106-R03941007-1.pdf: 6164912 bytes, checksum: 4a93dd29f39a72757ac79bba8d8e555d (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iv ABSTRACT v CONTENTS vi LIST OF FIGURES viii LIST OF TABLES xviii Chapter 1 Introduction 1 1.1 Motivation 1 1.2 Thesis Scope 3 Chapter 2 Background Knowledge 5 2.1 Histopathological Characteristics of Solar Lentigines 5 2.2 Evaluation Methods on Laser Treatments Concerning Solar Lentigines 9 2.3 Principles and Applications of Harmonic Generation Biopsy 11 2.4 Optical System Setup 13 Chapter 3 Materials and Methods 18 3.1 Subjects of the Clinical Study 18 3.2 Procedure of Image Acquisition 19 3.3 Quantification 22 3.3.1 Melanin Mass Density in Cytoplasm of Basal Cells at the Dermoepidermal Junction 24 3.3.2 2D Melanin-rich Cell Density 37 3.3.3 En Face Viewed 2D Basal Cell Size 49 3.3.4 Longitudinal 2D Basal Cell Size 50 3.3.5 Thickness of Stratum Corneum 53 3.3.6 Depth of Dermal Papillae Zone 60 Chapter 4 Results and Explanations 63 4.1 Characteristics of Solar Lentigines in HGB Images 63 4.2 Laser Treatment Effects on Solar Lentigines in HGB Images 73 4.3 Laser Treatment Improvement Evaluation 85 Chapter 5 Discussion 97 5.1 Explanation of Data and Comparison with Previous Studies 97 5.2 Attempted Analyses Methods 101 5.3 Future Prospects 103 Chapter 6 Conclusion 106 APPENDIX—Determining Intrinsic THG Brightness Ratio 108 1.1 Introduction 108 1.2 Image Acquisition Conditions 108 1.3 Protocol on Obtaining Intrinsic Ratio 109 1.3.1 Previewing Phase 109 1.3.2 ROI Selecting Phase 110 1.3.3 Screening Phase 112 1.3.4 Processing Phase 112 1.3.5 Inspecting Phase 116 1.4 Results 116 1.5 References of the Appendix 116 REFERENCES 118 | |
dc.language.iso | en | |
dc.title | 利用倍頻顯微術對日光引致慢性皮膚傷害之特徵分析 | zh_TW |
dc.title | Using Harmonic Generation Biopsy to Characterize Sun-induced Chronic Damage of Skin | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 廖怡華(Yi-Hua Liao),李國君(Gwo-Giun Lee),張中興(Chung-Hsing Chang) | |
dc.subject.keyword | 倍頻顯微術 (HGM),曬斑 (solar lentigo),活體光學虛擬切片,醫療美容,治療效果評估, | zh_TW |
dc.subject.keyword | harmonic generation microscopy,solar lentigo,in vivo optical biospy,aesthetic medicine,therapeutic evaluation, | en |
dc.relation.page | 125 | |
dc.identifier.doi | 10.6342/NTU201603818 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-01-10 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 光電工程學研究所 | zh_TW |
顯示於系所單位: | 光電工程學研究所 |
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