窄頻紅外光發射器之設計與其在癌症治療之研究

Abstract

近年研究發現，紅外光可調控生物體的生理機能，活絡粒線體內的電子傳遞鏈，影響蛋白質含量和代謝，進而影響生物體成長形態。本論文主要是設計結合紅外光發射器之細胞培養箱，並探討癌症治療的過程中，癌細胞受不同波段紅外光照射和調控，其抑癌的效果與機制。 本文的第一個主題，是探討 3~5 um窄頻中紅外光影響肺癌細胞 A549的療效。利用三層結構 (金/二氧化矽/金)的波導熱輻射發射器(Waveguide thermal emitter)，其上下兩層金之間的二氧化矽激發共振腔膜態產生窄頻紅外光源，可抑制肺癌細胞 A549生長，造成癌細胞粒線體膜電位的異常。 本文的第二個主題，是探討 3~5 um窄頻中紅外光影響 Paclitaxel對子宮頸癌細胞HeLa的療效。利用波導熱輻射發射器產生窄頻紅外光源照射子宮頸癌細胞 HeLa，可提升 Paclitaxel的治療效果，破壞粒線體膜電位，刺激下游的凋亡訊息路徑，造成更多子宮頸癌細胞 HeLa死亡。 本文的第三個主題，是探討 810 nm近紅外光雷射結合光動力療法對骨癌細胞 MG-63的療效。研究發現 810 nm近紅外光雷射可影響細胞的電子傳遞鏈和產生大量ATP，造成癌細胞吸收更多標靶於溶酶體的 Mono-L-aspartyl chlorin(e6) (NPe6)，接著由紅光激發光敏劑引起光動力效應，產生大量活性氧毒殺癌細胞。 本文的研究成果可讓我們了解，利用紅外光適當調控生物體的生理機能，結合癌症治療，可以加強抑制惡性腫瘤細胞。未來也可應用於臨床和家庭醫療，透過非侵入性、低副作用的特性，可減少傷害正常人體組織，達成更好的功效。

Recent studies found that infrared radiation (IR) can regulate physiology of organisms, stimulate mitochondrial electron transport chain, and affect expression of the genes, thereby regulating the morphology of cell growth. The goal of this thesis is to design the cell incubator equipped with IR emitter, and investigate the effectiveness and mechanisms of different wavelengths of IR on the cancer therapy. In the first topic of this thesis, it was clarified that the effect of mid-infrared radiation (MIR) with the peak wavelengths of 3~5 um range on the growth of lung cancer cell A549. The tri-layers Au/SiO2/Au waveguide thermal emitter (WTE) was used as the light source of narrow band IR. IR was originated from the resonance of cavity mode generated in the SiO2 layer between two Au films. The results showed that IR can inhibit the growth of cancer cell A549, leading to the collapse of mitochondrial membrane potential. In the second topic of this thesis, it was investigated that the effects of HeLa cells treated with Paclitaxel combined with 3~5 um MIR. Narrow band IR from WTE can promote the effectiveness of Paclitaxel through the collapse of mitochondrial membrane potential. In the third topic of this thesis, it was investigated that the effects of combination treatment of 810 nm near infrared radiation (NIR) laser and photodynamic therapy (PDT) in osteosarcoma cancer cell MG-63. The data showed that 810 nm NIR laser can regulate the electron transport chain and generate a large amount of ATP to enhance the uptake of lysosome-localized photosensitizer mono-L-aspartyl chlorin (e6) (NPe6). Therefore, overproduction of reactive oxygen species was activated through red light-induced PDT effects. The results of this thesis imply that the combination of cancer therapy and IR-regulated physiological activity can enhance the therapeutic effects on cancer cells. In future, IR can be applied to clinical and home health care to achieve therapeutic purposes without damaging normal tissue based on the characteristics of non-invasive and low side effects.