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Title: | 用於汞離子與過氧化氫感測之螢光金屬奈米團簇 Fluorescent Metal Nanocluster for Sensing of Mercury(II) and Hydrogen Peroxide |
Authors: | Ying-Chieh Chen 陳英傑 |
Advisor: | 張煥宗 |
Keyword: | 銀奈米團簇,螢光,去氧核糖核酸,三磷酸腺?,二價汞離子,邏輯閘,金奈米團簇,活性氧化物質,過氧化氫, Ag NCs,Fluorescence,DNA,ATP,Hg2+,Logic gate,Au NCs,ROS,H2O2, |
Publication Year : | 2015 |
Degree: | 碩士 |
Abstract: | 本篇論文可區分為兩部份。在第一部分,我們使用去氧核糖核酸 (DNA) 做為模板來合成銀奈米團簇。在pH為3.0的環境下,此銀奈米團簇的螢光強度會因有一定濃度的ATP存在而增強,然而它又會受到二價汞離子的存在影響而消光使螢光強度降低,我們便利用此特性做汞離子的檢測。在10-100 nM與100-1000 nM的汞存在下與銀奈米團簇的螢光強度具有斜率不同的線性關係。此螢光強度下降的現象分別是源自於因形成T-Hg2+-T造成整段模板DNA的構形改變以及銀汞齊的形成。這個方法可以達到1.7奈莫耳濃度的偵測極限,也成功地以湖水做為真實樣品進行檢測。另外,鑑於此銀奈米團簇的螢光強度可受調控,我們也將此銀奈米團簇應用於邏輯閘的發展。而在第二部分,我們以一個簡單的直接加熱法來合成具有雙波長螢光之牛血清白蛋白鈰/金奈米團簇,並利用它進行活性氧化物質如過氧化氫或次氯酸鈉的偵測。偵測的方式源於在410奈米波長之螢光對活性氧化物質的存在無明顯反應,而680奈米波長之螢光則會產生消光的現象。便可以利用410奈米波長之螢光做為內標準,而680奈米波長螢光之消長做為訊號來達到偵測的目的。另一方面,由於雙波長螢光分別對於pH值和過氧化氫有響應,故可以利用質子濃度及過氧化氫濃度為輸入,而螢光訊號為輸出來建立邏輯閘。 The thesis is constructed by two parts. In the first part, several DNA templates have been used to prepare DNA-template silver nanoclusters (DNA-Ag NCs). At pH 3.0, the fluorescence intensity of as-prepared DNA-Ag NCs is enhanced by ATP and the enhanced fluorescence is quenched by Hg2+. Utilizing the special properties, a method to detect Hg2+ was developed. The fluorescence response over the Hg2+ concentration shows two linear ranges over 10−100 and 100−1000 nM, mainly because the fluorescence quenching as a result of DNA conformational changes are through the T-Hg2+-T coordination and the formation of amalgam with Ag NCs, separately. Having a good sensitivity for Hg2+ (down to 1.7 nM), the T15-DNA-Ag NCs probe has been validated for the analysis of Hg2+ in spiked pond water. Based on the switch–on and –off fluorescence properties of T15-DNA-Ag NCs, an IMPLICATION logic gate has been fabricated using the concentrations of ATP and Hg2+ as inputs and the fluorescence intensity at 585 nm as an output. In the second part, ratiometric detection of reactive oxygen species (ROS) such as hydrogen peroxide and sodium hypochlorite has been realized using dual-emitting bovine serum albumin (BSA) template cerium/gold nanoclusters (BSA-Ce/Au NCs), synthesized using a simple direct heating method. The emission at 410 nm is relatively insensitive to the presence of ROS, while the fluorescence intensity of the peak at 680 nm is quenched by the addition of ROS. Moreover, through the control of two inputs – pH values and hydrogen peroxide concentration, and the different response of the two fluorescence peaks, IMP logic gates could be developed. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54404 |
Fulltext Rights: | 有償授權 |
Appears in Collections: | 化學系 |
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