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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 潘敏雄 | |
dc.contributor.author | Ying-Yi Chen | en |
dc.contributor.author | 陳盈伊 | zh_TW |
dc.date.accessioned | 2021-06-15T16:43:44Z | - |
dc.date.available | 2017-08-16 | |
dc.date.copyright | 2015-08-16 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-10 | |
dc.identifier.citation | 行政院農業委員會, 藥用植物主題館-薑黃. 2014.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/53089 | - |
dc.description.abstract | 肥胖是由於體內脂肪組織過度堆積所致,並與代謝疾病密切相關。脂肪細胞新生作用 (Adipogenesis) 是指前脂肪細胞分化變成脂肪細胞的過程,會使脂肪細胞的數目增加和體積變大,與肥胖的形成有關,因此抑制脂肪細胞新生作用可視為抗肥胖之新策略。類薑黃素 (Curcuminoids) 為薑黃根莖中具有生物活性的成份,其主要包括薑黃素 (Curcumin, Cur)、去甲氧基薑黃素 (Demethoxycurcumin, DMC) 和雙去甲氧基薑黃素 (Bis-demethoxycurcumin, BDMC)。本研究先以3T3-L1前脂肪細胞模式評估類薑黃素對脂肪細胞新生作用之影響,據Oil red O染色結果顯示,在25 μM濃度下,類薑黃素皆能顯著抑制細胞內脂質的累積,且抑制效果BDMC > DMC > Cur,再進而探討BDMC對3T3-L1 前脂肪細胞分化過程之影響及分子作用機轉。研究顯示BDMC能抑制前脂肪細胞分化成脂肪細胞,其主要是作用在分化前期之細胞擴增 (Mitotic clonal expansion, MCE) 階段,使細胞週期延遲或停滯。且發現BDMC是藉由下調Mitogen-activated protein kinases (MAPK) pathways達到抑制MCE的作用。在經BDMC處理後,會降低Cyclin A和Cyclin B之蛋白質表現量,增加p21之蛋白質表現量,使細胞週期停滯於G0/G1期,並降低脂肪細胞分化相關之重要轉錄因子PPARγ和C/EBPα的蛋白質表現,進而抑制脂肪細胞分化之進程,最終達到減少脂質累積之功效。此外,以高脂飼料誘導C57BL/6小鼠肥胖之動物實驗模式,評估BDMC降低體脂肪生成之效用。實驗結果顯示,於高脂飲食中添加0.5 % (w/w) BDMC能顯著減緩體重上升,改善脂肪組織堆積情形,且由切片染色結果可發現餵食BDMC能抑制脂肪細胞體積增大之效果。綜合上述結果顯示,BDMC具有抑制脂肪新生作用之功效,並能改善高脂飼料誘導之肥胖,能減緩體脂肪生成,具有開發成不易形
成體脂肪之保健食品的潛力。 | zh_TW |
dc.description.abstract | Obesity is caused by excessive accumulation of body fat, which is closely related to complex metabolic diseased. Adipogenesis is a key process in determining the number and size of mature adipocytes in the development of obesity. Curcumin (Cur), has been reported to inhibit on adipocyte differentiation. But other curcuminoids present in turmeric, demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC) have not been investigated whether they exhibit anti-adipogenic activity. Here, we investigated the effects of curcuminoids on adipogenesis and the molecular mechanisms in adipocyte differentiation. Our Oil Red O staining result revealed that 25 μM curcuminoids effectively suppressed accumulation of lipid in adipocytes. And BDMC may exerts more potent anti-adipogenic function than DMC and Cur. This anti-adipogenic function of BDMC was largely inhibited the early stage of adipogenesis through prevention of mitotic clonal expansion (MCE). We found that BDMC suppressed MCE was accompanied by reducing the mitogen-activated protein kinase (MAPK) signaling pathways activation. In particular, BDMC blocked the cell cycle at G0/G1 phase by regulating the protein expression of cyclin A, cyclin B and p21. Also, BDMC reduced the protein expression level of PPARγ and C/EBPα. Furthermore, we examined the anti-obesity effects of BDMC on obesity animal model. C57BL/6 mice were fed high-fat diet (HFD) and BDMC at same time. The result showed that the HFD supplement with 0.5 % (w/w) BDMC had significantly lower body weight gain and less adipose tissue mass. H & E staining result showed that the mice were fed BDMC could inhibit hypertrophy in adipocyte. In conclusion, BDMC had anti-adipogenic effect in vitro and anti-obesity in vivo. Our results suggested that BDMC has potential to be an anti-obesity functional food. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T16:43:44Z (GMT). No. of bitstreams: 1 ntu-104-R02641029-1.pdf: 5180589 bytes, checksum: 921638dfe34a1145300a6e2fe6483573 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 謝誌 I
中文摘要 III Abstract IV 目錄 V 附圖目錄 IX 圖目錄 X 附表目錄 XI 縮寫表 XIII 第一章、緒論 1 第二章、文獻回顧 2 第一節、肥胖 2 (一) 簡介 2 (二) 肥胖定義標準 2 (三) 肥胖的成因 3 (四) 肥胖與疾病 4 (五) 肥胖症的預防及治療 6 第二節、脂肪組織 7 (一) 簡介 7 (二) 脂肪細胞生命週期 9 (三) 脂肪組織的代謝功能 10 (四) 脂肪組織的內分泌功能 13 第三節、脂肪細胞新生作用 17 (一)簡介 17 (二) 3T3-L1前脂肪細胞分化 17 (三) 脂肪細胞分化之調控因子 20 第四節、細胞週期蛋白在脂肪細胞新生作用的影響 27 (一) 細胞週期 27 (二) 細胞週期的調控 28 (三) 細胞週期蛋白在MCE的調控 29 第五節、脂肪細胞新生作用過程中訊息傳遞路徑間之調控機制 32 (一) 簡介 32 (二) 胰島素在脂肪細胞之訊息傳遞路徑 32 (三) MAPK pathway在脂肪細胞分化之調控機制 33 第六節、薑黃及類薑黃素 36 (一) 薑黃 36 (二) 類薑黃素 37 (三) 去二甲氧基薑黃素 (BDMC) 之研究 38 第三章、實驗目的與架構 39 第一節、研究動機與目的 39 第二節、實驗架構 40 第四章、材料與方法 41 第一節、實驗材料 41 (一) 儀器設備 41 (二) 藥品試劑 42 第二節、細胞實驗方法 45 (一) 樣品製備 45 (二) 細胞培養 45 (三) 細胞分化 47 (四) 油紅染色 48 (五) MTT細胞存活率試驗 50 (六) Trypan blue細胞存活率試驗 51 (七) 脂質分解作用分析 51 (八) 流式細胞儀分析-PI染色 52 (八) 蛋白質萃取 53 (九) 蛋白質定量 54 (十) 西方墨點法 54 第三節、動物實驗方法 57 (一) 實驗動物飼養 57 (二) 飼料配製 58 (三) 實驗動物犧牲 59 (四) 血液生化質測定 60 (五) 組織包埋與切片 61 第四節、統計分析 63 第五章、結果與討論 64 第一節、細胞實驗 64 (一) BDMC抑制3T3-L1脂肪細胞內脂質堆積的情形 64 (二) 比較類薑黃素抑制3T3-L1脂肪細胞內脂質堆積的能力 64 (三) BDMC 對3T3-L1脂肪細胞存活率之影響 65 (四) BDMC對成熟脂肪細胞脂解作用之影響 67 (五) 在不同分化時間點BDMC對3T3-L1脂肪細胞內脂質堆積之影響 67 (六) BDMC對3T3-L1脂肪細胞分化期間細胞週期之影響 68 (七) BDMC對脂肪細胞分化轉錄因子之影響 69 (八) BDMC 對細胞擴增期 (MCE) 之細胞週期蛋白質表現量之影響 70 (九) BDMC 對MAPK pathway和PI3K-AKT pathway之影響 71 第二節、動物實驗 73 (一) 餵食BDMC對C57BL/6小鼠體重之影響 73 (二) BDMC對C57BL/6小鼠攝食量之影響 74 (三) BDMC對體脂肪重量之影響 75 (四) BDMC對體脂肪細胞大小之影響 76 (五) 血清生化值檢測 76 第六章、結論 79 第七章、圖表 81 第八章、參考文獻 99 | |
dc.language.iso | zh-TW | |
dc.title | Bis-demethoxycurcumin (BDMC) 抑制脂肪細胞新生作用
及減緩高脂飼料誘導之肥胖 | zh_TW |
dc.title | Bis-demethoxycurcumin (BDMC) inhibits adipogenesis in 3T3-L1 preadipocytes and suppresses obesity in high-fat diet-feeding | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 游若? | |
dc.contributor.oralexamcommittee | 何其儻,何元順,李銘仁 | |
dc.subject.keyword | 肥胖,脂肪細胞新生作用,雙去甲氧基薑黃素 (BDMC),高脂飼料,細胞擴增期 (MCE), | zh_TW |
dc.subject.keyword | obesity,adipogenesis,bisdemethoxycurcumin (BDMC),high-fat diet,Mitotic clonal expansion (MCE), | en |
dc.relation.page | 111 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-08-10 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
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