請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52968
標題: | 水體碳氮比對益生菌及副溶血弧菌生長、水質與相互抑制之影響 Effects of C/N ratios on Growth, Water Quality and Mutual Inhibition of Probiotic and Vibrio parahaemolyticus |
作者: | Tai-Yun Kao 高岱筠 |
指導教授: | 陳秀男 |
關鍵字: | 碳氮比,枯草桿菌,光合菌,副溶血弧菌,生長,水質,抑制, Carbon/ Nitrogen Ratios,Bacillus subtilis,Rhodobacter sphaeroides,Vibrio parahaemolyticus,Growth,Water quality,Inhibition, |
出版年 : | 2015 |
學位: | 碩士 |
摘要: | 本實驗將糖蜜作為碳源添加至養殖水中,使水體碳氮比分別為0, 5, 10, 15, 20五種比例,0為無添加碳源組別,作為控制組。探討不同碳氮比條件對養殖環境中枯草桿菌 (Bacillus subtilis)、光合菌 (Rhodobacter sphaeroides) 及副溶血弧菌 (Vibrio parahaemolyticus) 生長、水質處理及相互抑制之影響。
實驗結果顯示枯草桿菌於碳氮比20組,生長顯著高於控制組 (p < 0.05);添加碳源的處理組,皆有效提升光合菌生長,與控制組有顯著差異 (p < 0.05),其中以碳氮比15組有最高菌量;副溶血弧菌於碳氮比5組生長較快,與控制組有顯著差異 (p < 0.05)。水質處理的結果顯示枯草桿菌在各處理組皆能降低氨-氮濃度,各組間無顯著差異 (p > 0.05),亞硝酸-氮濃度於碳氮比20組顯著降低,與控制組有顯著差異 (p < 0.05);光合菌於添加碳源的組別,氨-氮與亞硝酸-氮濃度皆下降,其中以碳氮比10、15、20組,水中氨-氮與亞硝酸-氮濃度顯著低於控制組 (p < 0.05);副溶血弧菌於碳氮比15、20組,水中氨-氮與亞硝酸-氮濃度顯著低於控制組 (p < 0.05)。抑制實驗中枯草桿菌103、105 cfu/ ml添加量對於副溶血弧菌103 cfu/ ml有抑制生長效果,光合菌105 cfu/ ml添加量對於副溶血弧菌103 cfu/ ml,第72小時後有抑制生長效果。 添加碳源提高水體中碳氮比,能有效促進水中細菌對水質的改善,並且營造較適合枯草桿菌和光合菌的環境,達到抑制副溶血弧菌生長。 This study added molasses as carbon source into the water to control carbon/ nitrogen ratios at five levels, 0, 5, 10, 15 and 20. 0 added nothing as a control group. To investigate different carbon/ nitrogen ratios effects of Bacillus subtilis, Rhodobacter sphaeroides and Vibrio parahaemolyticus on growth, water quality and mutual inhibition. In the experiment of the bacteria growth in different carbon/ nitrogen ratios water, the results show that Bacillus subtilis at 20 group were significantly higher than the control (p < 0.05). Each treatment group increased growth of Rhodobacter sphaeroides significantly compared to the control group (p < 0.05), while 15 treatment group had better effect than all treatment group. In addition, Vibrio parahaemolyticus at 5 treatment group were significantly increased rate of growth to control (p < 0.05). In the experiment of the water quality of bacteria in different carbon/ nitrogen ratios water, the results indicated that Bacillus subtilis reduced ammonia-N concentrations in water, but it was not effects significantly on each treatment group. In addition, the nitrite-N concentrations significantly lower at 20 treatment group than control (p < 0.05). Each treatment group of Rhodobacter sphaeroides, which reduced ammonia-N and nitrite-N concentration significantly compared to the control group (p < 0.05), while 10、15 and 20 treatment had better results. Vibrio parahaemolyticus at 15、20 treatment reduce the ammonia-N and nitrite-N concentrations significantly than control group (p < 0.05). Through the inhibition of bacteria growth, 103、105 cfu/ ml of Bacillus subtilis can produce inhibition effects on 103 cfu/ ml Vibrio parahaemolyticus. The 105 cfu/ ml of Rhodobacter sphaeroides had inhibition effects on 103 cfu/ ml Vibrio parahaemolyticus after 72 hr. In conclusion of this study, carbon source addition increase carbon/ nitrogen ratios in water can effectively improve the water quality and growth activities of Bacillus subtilis and Rhodobacter sphaeroides. To build a suitable environment of probiotic for more efficiently inhibit the growth of Vibrio parahaemolyticus. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52968 |
全文授權: | 有償授權 |
顯示於系所單位: | 漁業科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-104-1.pdf 目前未授權公開取用 | 1.88 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。