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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳秀男 | |
| dc.contributor.author | Tai-Yun Kao | en |
| dc.contributor.author | 高岱筠 | zh_TW |
| dc.date.accessioned | 2021-06-15T16:36:40Z | - |
| dc.date.available | 2019-08-20 | |
| dc.date.copyright | 2015-08-20 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-12 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/52968 | - |
| dc.description.abstract | 本實驗將糖蜜作為碳源添加至養殖水中，使水體碳氮比分別為0, 5, 10, 15, 20五種比例，0為無添加碳源組別，作為控制組。探討不同碳氮比條件對養殖環境中枯草桿菌 (Bacillus subtilis)、光合菌 (Rhodobacter sphaeroides) 及副溶血弧菌 (Vibrio parahaemolyticus) 生長、水質處理及相互抑制之影響。
實驗結果顯示枯草桿菌於碳氮比20組，生長顯著高於控制組 (p < 0.05)；添加碳源的處理組，皆有效提升光合菌生長，與控制組有顯著差異 (p < 0.05)，其中以碳氮比15組有最高菌量；副溶血弧菌於碳氮比5組生長較快，與控制組有顯著差異 (p < 0.05)。水質處理的結果顯示枯草桿菌在各處理組皆能降低氨-氮濃度，各組間無顯著差異 (p > 0.05)，亞硝酸-氮濃度於碳氮比20組顯著降低，與控制組有顯著差異 (p < 0.05)；光合菌於添加碳源的組別，氨-氮與亞硝酸-氮濃度皆下降，其中以碳氮比10、15、20組，水中氨-氮與亞硝酸-氮濃度顯著低於控制組 (p < 0.05)；副溶血弧菌於碳氮比15、20組，水中氨-氮與亞硝酸-氮濃度顯著低於控制組 (p < 0.05)。抑制實驗中枯草桿菌103、105 cfu/ ml添加量對於副溶血弧菌103 cfu/ ml有抑制生長效果，光合菌105 cfu/ ml添加量對於副溶血弧菌103 cfu/ ml，第72小時後有抑制生長效果。 添加碳源提高水體中碳氮比，能有效促進水中細菌對水質的改善，並且營造較適合枯草桿菌和光合菌的環境，達到抑制副溶血弧菌生長。 | zh_TW |
| dc.description.abstract | This study added molasses as carbon source into the water to control carbon/ nitrogen ratios at five levels, 0, 5, 10, 15 and 20. 0 added nothing as a control group. To investigate different carbon/ nitrogen ratios effects of Bacillus subtilis, Rhodobacter sphaeroides and Vibrio parahaemolyticus on growth, water quality and mutual inhibition.
In the experiment of the bacteria growth in different carbon/ nitrogen ratios water, the results show that Bacillus subtilis at 20 group were significantly higher than the control (p < 0.05). Each treatment group increased growth of Rhodobacter sphaeroides significantly compared to the control group (p < 0.05), while 15 treatment group had better effect than all treatment group. In addition, Vibrio parahaemolyticus at 5 treatment group were significantly increased rate of growth to control (p < 0.05). In the experiment of the water quality of bacteria in different carbon/ nitrogen ratios water, the results indicated that Bacillus subtilis reduced ammonia-N concentrations in water, but it was not effects significantly on each treatment group. In addition, the nitrite-N concentrations significantly lower at 20 treatment group than control (p < 0.05). Each treatment group of Rhodobacter sphaeroides, which reduced ammonia-N and nitrite-N concentration significantly compared to the control group (p < 0.05), while 10、15 and 20 treatment had better results. Vibrio parahaemolyticus at 15、20 treatment reduce the ammonia-N and nitrite-N concentrations significantly than control group (p < 0.05). Through the inhibition of bacteria growth, 103、105 cfu/ ml of Bacillus subtilis can produce inhibition effects on 103 cfu/ ml Vibrio parahaemolyticus. The 105 cfu/ ml of Rhodobacter sphaeroides had inhibition effects on 103 cfu/ ml Vibrio parahaemolyticus after 72 hr. In conclusion of this study, carbon source addition increase carbon/ nitrogen ratios in water can effectively improve the water quality and growth activities of Bacillus subtilis and Rhodobacter sphaeroides. To build a suitable environment of probiotic for more efficiently inhibit the growth of Vibrio parahaemolyticus. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T16:36:40Z (GMT). No. of bitstreams: 1 ntu-104-R02b45008-1.pdf: 1929301 bytes, checksum: 54335593b85f3822367d13668a796819 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 中文摘要 II
Abstract III 目錄 V 圖目錄 VIII 前言 1 文獻整理 3 一、碳氮比 3 二、微生物營養 3 2.1碳源： 3 2.2氮源： 4 三、碳氮比調控的應用 4 四、益生菌在水產養殖的應用 6 五、實驗使用之益生菌 8 5.1枯草桿菌 8 5.2光合菌 10 六、養殖環境中有害物質成因與影響 10 七、蝦類早期死亡綜合症 (Shrimp Early Mortality Syndrome；EMS) 11 7.1病原 12 7.2致病機制 12 八、糖蜜 13 材料與方法 14 一、菌株之培養 14 1.1菌株來源 14 1.2培養基的選擇 14 1.3菌種保存 14 1.4菌落計數 (塗佈法) 15 1.5生長曲線測定 15 1.6工作菌液製作 15 二、實驗試水來源 16 2.1 養殖汙水 16 2.2 水中碳氮比檢測 16 2.3 實驗試水配製 16 三、枯草桿菌、光合菌和副溶血弧菌在不同碳氮比水中生長之影響 17 3.1實驗操作 17 四、枯草桿菌、光合菌和副溶血弧菌在不同碳氮比水中水質檢測 17 4.1測量水質的試水來源 17 4.2 氨-氮濃度的測試 17 4.3亞硝酸-氮濃度的測試 18 五、枯草桿菌及光合菌在不同碳氮比水中對副溶血弧菌抑制檢測 19 5.1 總生菌數測定 19 5.2 弧菌數測定 19 5.3 實驗操作 19 六、統計分析 20 結果 21 一、枯草桿菌、光合菌和副溶血弧菌在不同碳氮比水中生長之影響 21 1.1不同碳氮比對枯草桿菌生長之影響 21 1.2不同碳氮比對光合菌生長之影響 21 1.3不同碳氮比對副溶血弧菌生長之影響 21 二、枯草桿菌、光合菌和副溶血弧菌在不同碳氮比水中水質檢測 22 2.1枯草桿菌在不同碳氮比水中氨-氮濃度影響 22 2.2枯草桿菌在不同碳氮比水中亞硝酸-氮濃度影響 22 2.3光合菌在不同碳氮比水中氨-氮濃度影響 22 2.4光合菌在不同碳氮比水中亞硝酸-氮濃度影響 23 2.5副溶血弧菌在不同碳氮比水中氨-氮濃度影響 23 2.6副溶血弧菌在不同碳氮比水中亞硝酸-氮濃度影響 23 三、枯草桿菌及光合菌在不同碳氮比水中對副溶血弧菌抑制檢測 24 3.1枯草桿菌103 cfu/ ml與副溶血弧菌103 cfu/ ml 24 3.2枯草桿菌105 cfu/ ml與副溶血弧菌103 cfu/ ml 24 3.3光合菌105 cfu/ ml與副溶血弧菌103 cfu/ ml 24 討論 26 一、枯草桿菌、光合菌和副溶血弧菌在不同碳氮比水中生長之影響 26 二、枯草桿菌、光合菌和副溶血弧菌在不同碳氮比水中水質檢測 27 三、枯草桿菌及光合菌在不同碳氮比水中對副溶血弧菌抑制檢測 28 結論 31 一、枯草桿菌、光合菌和副溶血弧菌在不同碳氮比水中生長之影響 31 二、枯草桿菌、光合菌和副溶血弧菌在不同碳氮比水中水質檢測 31 三、枯草桿菌及光合菌在不同碳氮比水中對副溶血弧菌抑制檢測 31 參考文獻 32 圖表附件 49 附錄 71 | |
| dc.language.iso | zh-TW | |
| dc.subject | 枯草桿菌 | zh_TW |
| dc.subject | 碳氮比 | zh_TW |
| dc.subject | 光合菌 | zh_TW |
| dc.subject | 水質 | zh_TW |
| dc.subject | 抑制 | zh_TW |
| dc.subject | 副溶血弧菌 | zh_TW |
| dc.subject | 生長 | zh_TW |
| dc.subject | Inhibition | en |
| dc.subject | Bacillus subtilis | en |
| dc.subject | Rhodobacter sphaeroides | en |
| dc.subject | Vibrio parahaemolyticus | en |
| dc.subject | Growth | en |
| dc.subject | Water quality | en |
| dc.subject | Carbon/ Nitrogen Ratios | en |
| dc.title | 水體碳氮比對益生菌及副溶血弧菌生長、水質與相互抑制之影響 | zh_TW |
| dc.title | Effects of C/N ratios on Growth, Water Quality and Mutual Inhibition of Probiotic and Vibrio parahaemolyticus | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 廖文亮,劉秉忠,冉繁華 | |
| dc.subject.keyword | 碳氮比,枯草桿菌,光合菌,副溶血弧菌,生長,水質,抑制, | zh_TW |
| dc.subject.keyword | Carbon/ Nitrogen Ratios,Bacillus subtilis,Rhodobacter sphaeroides,Vibrio parahaemolyticus,Growth,Water quality,Inhibition, | en |
| dc.relation.page | 72 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-12 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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