整合電化學阻抗分析晶片之微流道系統進行 整合電化學阻抗分析晶片之微流道系統進行全血樣本之血漿分離及其血紅素量測

Abstract

本論文提出了一套全血樣本處理微流道系統，包含血液處理的PMMA微流道晶片及電阻抗分析感測器。其中血液處理的PMMA微流道晶片可進行血球的去除以及血漿的混合，並由電阻抗頻譜感測器量測生物分子達到完整的血液檢測。本論文首先使用流體力學理論說明具有彎曲流道以及沉降凹槽之微流道晶片可達到稀釋血漿以及過濾紅血球的功能，接著提出使用精密雕刻機進行PMMA材質之微流道晶片製作，驗證系統量測血液生物分子之可行性部分，本論文使用電阻抗分析感測器進行血紅蛋白的量測。本論文之初期實驗結果證明此微流道系統可達成傳統儀器以及手動處理的血液前處理流程，相信未來整合微壓電幫浦和電阻抗分析感測器將可實現定點照護功能。

The paper presents a whole blood processing microfluidic system, consists of a PMMA microfluidic chip and an Electrochemical Impedance Spectroscopy (EIS) sensor. In this system, we used an engraving machine to fabricate a PMMA microfluidic chip. The microfluidic chip consists of two specific microstructural designs to perform efficient whole blood process. The first structure design is a series of curved microchannel to achieve blood plasma mixing, purification, dilution. The second structure design is a series of microtrench to achieve blood cell sedimentation. Finally, we used an EIS sensor to measure Hemoglobin from the blood sample after PMMA microfluidics process to prove the concept of this microfluidic system. Based on preliminary results in this thesis, we believe the system can eventually move toward to a simplified portable device for point-of-care applications.