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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃念祖(NIAN-ZU HUANG) | |
| dc.contributor.author | Jiun-Rue Lin | en |
| dc.contributor.author | 林俊叡 | zh_TW |
| dc.date.accessioned | 2021-06-15T13:59:46Z | - |
| dc.date.available | 2016-08-21 | |
| dc.date.copyright | 2015-08-21 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-20 | |
| dc.identifier.citation | 1 Heidi, S., Dave, S. B. H. & Klaus, P. Cell-free nucleic acids as biomarkers in cancer patients. Nature Reviews Cancer 11, 426-437, doi:10.1038/nrc3066 (2011).
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51960 | - |
| dc.description.abstract | 本論文提出了一套全血樣本處理微流道系統，包含血液處理的PMMA微流道晶片及電阻抗分析感測器。其中血液處理的PMMA微流道晶片可進行血球的去除以及血漿的混合，並由電阻抗頻譜感測器量測生物分子達到完整的血液檢測。本論文首先使用流體力學理論說明具有彎曲流道以及沉降凹槽之微流道晶片可達到稀釋血漿以及過濾紅血球的功能，接著提出使用精密雕刻機進行PMMA材質之微流道晶片製作，驗證系統量測血液生物分子之可行性部分，本論文使用電阻抗分析感測器進行血紅蛋白的量測。本論文之初期實驗結果證明此微流道系統可達成傳統儀器以及手動處理的血液前處理流程，相信未來整合微壓電幫浦和電阻抗分析感測器將可實現定點照護功能。 | zh_TW |
| dc.description.abstract | The paper presents a whole blood processing microfluidic system, consists of a PMMA microfluidic chip and an Electrochemical Impedance Spectroscopy (EIS) sensor. In this system, we used an engraving machine to fabricate a PMMA microfluidic chip. The microfluidic chip consists of two specific microstructural designs to perform efficient whole blood process. The first structure design is a series of curved microchannel to achieve blood plasma mixing, purification, dilution. The second structure design is a series of microtrench to achieve blood cell sedimentation. Finally, we used an EIS sensor to measure Hemoglobin from the blood sample after PMMA microfluidics process to prove the concept of this microfluidic system. Based on preliminary results in this thesis, we believe the system can eventually move toward to a simplified portable device for point-of-care applications. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T13:59:46Z (GMT). No. of bitstreams: 1 ntu-104-R02945047-1.pdf: 3384461 bytes, checksum: e1d388e2ca5b344b3b9e6ba15f14e287 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 第 1 章 緒論 1
1.1 研究背景 1 1.2 文獻回顧 2 1.2.1 血液分子檢測 2 1.2.2 被動式微混合器 5 1.2.3 電化學阻抗頻譜感測器 10 1.3 研究動機以及目的 11 1.4 論文架構 13 第 2 章 基本理論 14 2.1 血液處理 14 2.1.1 細胞沉降理論 14 2.1.2 微流體混合理論 16 2.1.3 電阻抗分析感測器量測原理 16 第 3 章 實驗架構 22 3.1 實驗架構介紹 22 3.2 血液處理之微流道晶片 22 3.2.1 PMMA晶片製作 25 3.2.2 微流道晶片接合以及EIS感測器接合 28 3.3 電化學阻抗分析感測器 29 3.4 量測架構介紹 30 第 4 章 實驗模擬分析 32 4.1 COMSOL 模擬軟體簡介 32 4.2 COMSOL 模型 33 4.2.1 血球沉降模型 33 4.3 數值模擬結果 34 4.3.1 粒子軌跡分析結果 34 第 5 章 實驗架設 36 5.1 血球沉降測試 36 5.2 色素判斷混合流道混合效率 37 5.3 吸光度測試微流道血液稀釋倍率 37 5.3.1 量測方法 38 5.3.2 全血稀釋倍率實驗 38 5.4 電化學阻抗分析量測 40 5.4.1 電化學阻抗分析量測方法 40 5.4.2 電化學量測流程 41 第 6 章 結果與討論 44 6.1 紅血球沉降分析 44 6.1.1 未裂解血液沉降分析 44 6.1.2 裂解血液沉降分析 45 6.2 微流道混合效率分析 46 6.3 標準化血液稀釋倍率 48 6.3.1 微孔盤分光光度計解析度確認 49 6.3.2 未裂解血液吸光度結果 49 6.3.3 裂解血液吸光度結果 50 6.4 電化學阻抗分析量測生物分子 53 6.4.1 連結抗體抗原之電阻抗量測結果 53 6.4.2 手動稀釋裂解血液量測 55 6.4.3 微流道處理樣品之電化學阻抗分析量測結果 56 第 7 章 結論與未來展望 59 7.1 結論 59 7.2 未來展望 60 | |
| dc.language.iso | zh-TW | |
| dc.subject | 血紅素 | zh_TW |
| dc.subject | 全血樣本 | zh_TW |
| dc.subject | 血漿純化 | zh_TW |
| dc.subject | whole blood | en |
| dc.subject | Hempglobin | en |
| dc.subject | plasma purification | en |
| dc.title | 整合電化學阻抗分析晶片之微流道系統進行
整合電化學阻抗分析晶片之微流道系統進行全血樣本之血漿分離及其血紅素量測 | zh_TW |
| dc.title | A Microfluidic Device Integrated with Electrochemical Impedance Spectroscopy Sensor for Whole Blood Plasma Separation and Hemoglobin Detection | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林致廷(ZHI-TING LIN),董奕鍾(YI-ZHONG DONG),許聿翔(YU-XIANG XU) | |
| dc.subject.keyword | 全血樣本,血漿純化,血紅素, | zh_TW |
| dc.subject.keyword | whole blood,plasma purification,Hempglobin, | en |
| dc.relation.page | 63 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-08-20 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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