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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 羅翊禎(Yi-Chen Lo) | |
dc.contributor.author | Shasha Zhuang | en |
dc.contributor.author | 莊莎莎 | zh_TW |
dc.date.accessioned | 2021-06-15T13:28:30Z | - |
dc.date.available | 2021-02-01 | |
dc.date.copyright | 2016-02-25 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-02-08 | |
dc.identifier.citation | 北京大學安康藥物研究院. 一種絞股藍總皂甙的制取方法. 中國專利: CN1317295C, 20070523.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/51248 | - |
dc.description.abstract | 羅漢果,學名Siraitia grosvenorii,作為一種傳統中醫藥材常被用來治療感冒、咳嗽、咽喉炎、哮喘、支气管炎和腸胃不適等疾病。各項研究顯示,羅漢果的主要活性成分為一類三萜類皂苷,稱羅漢果皂苷,有如抗氧化、抗病毒、抗癌、抗發炎及抗糖尿病等多種生物活性,在新鮮的羅漢果果實中約占1%,主要以mogroside V的形式存在。羅漢果皂苷還有高甜度、低熱量的特點,可作為天然甜味劑有極強的優勢。由於原料來源及純化技術的限制,過去的研究難以涉及各種羅漢果皂苷間的功效區別。本研究利用不同的方式開發了不同醣基型式羅漢果皂苷之製備方法。用酵母菌Saccharomyces cerevisiae發酵羅漢果果實萃取物,將羅漢果皂苷生物轉化成帶三個醣基的mogroside III E,再以HP-20大孔吸附樹脂管柱對發酵產物進行分離純化,得到純度達54.19%的mogroside III E產品,回收率為72.23%,並成功實現了10倍擴大純化。另外利用化學法將羅漢果萃取物置於2 N鹽酸中在85℃條件下進行酸水解產生皂苷元mogrol,透過乙酸乙酯-水分液萃取法取得純度為13.40%的mogrol產品。本研究中亦利用靈芝菌株ATCC 76537和ATCC 76538將羅漢果皂苷轉化成帶兩個醣基的mogroside II A,進一步延長發酵時間可得到皂苷元mogrol。未來將可利用高純度之各種羅漢果皂苷進行生物利用率等研究。 | zh_TW |
dc.description.abstract | Lo Han Kuo (LHK), Siraitia grosvenorii, has been used as traditional Chinese herbal medicine to treat colds, cough, sore throats, asthma, bronchitis, stomach and intestinal discomfort. The main bioactive compounds of LHK are triterpenoid saponins, called mogrosides, making up about 1% of fresh fruits, in which mogroside V is the major form. Limited studies demonstrated that mogrosides extracts have various bioactivities, such as antioxidant, anticancer, antivirus, anti-inflammatory and antidiabetes. Studies into the bioactivities of specific mogrosides have been hindered by limit of sources and techniques for purifying these complex chemicals. It has been found that mogroside V could be transformed into other glycosyl-type mogrosides by biologic and chemical methods. The objective of this study is to find the ways to prepare and purify different mogrosides. Mogrosides in LHK extracts were biotransformed into mogroside III E by Saccharomyces cerevisiae, fermentation product was purified by HP-20 resin column to get enriched mogroside III E (54.19%), and the recovery rate for mogroside III E was 72.23%. Mogrol was produced via acid hydrolysis in 2 N HCl at 85℃. Thirteen percents of mogrol was collected with EA-H2O partition of acid hydrolysis products. In addition, mogroside II A and mogrol could be obtained using Ganoderma lucidum ATCC 76537 or ATCC 76538. In conclusion, individual mogroside production can be achieved using various microbial fermentation for future application. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T13:28:30Z (GMT). No. of bitstreams: 1 ntu-105-R02641040-1.pdf: 93108107 bytes, checksum: 26e80d7f4d66527c762c4a8a5c94812f (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 摘要 I
Abstract II 總目錄 III 圖目錄 VI 表目錄 VII 附錄目錄 VIII 壹、前言 1 贰、文獻回顧 2 一、羅漢果(Siraitia grosvenorii) 2 (一)主要成分 3 (二)安全性評估 4 (三)應用與加工產品 5 二、羅漢果皂苷(mogrosides) 6 (一)化學結構 6 (二)含量及感官特性 10 (三)分析方法 12 (四)生物活性 17 (五)消化與代謝 20 三、 羅漢果皂苷的生物轉化 24 (一)釀酒酵母對羅漢果皂苷的生物轉化 24 (二)靈芝對羅漢果皂苷的生物轉化 25 四、皂苷的酸水解 29 (一)酸水解的基本原理 29 (二)酸水解的影響因素 30 (三)皂苷的酸水解 31 五、大孔吸附樹脂(macroporous resin) 33 (一)大孔吸附樹脂簡介 33 (二)分離純化影響因素 33 (三)應用於天然產物的分離純化 35 叁、研究目的及實驗架構 38 一、研究目的 38 二、實驗框架 39 肆、材料與方法 40 一、實驗材料 40 二、實驗藥品 40 (一)標準品 40 (二)化學試劑及藥品 40 (三)微生物培養材料 41 三、儀器設備 41 (一)實驗耗材 41 (二)柱層析設備 42 (三)液相層析與質譜串聯系統 42 (四)一般儀器設備 43 四、實驗方法 44 (一)固相萃取純化羅漢果皂苷 44 (二)液相層析串聯質譜分析羅漢果皂苷 44 (三)酵母菌生產mogroside III E 47 (四)HP-20樹脂分離純化mogroside III E 48 (五)羅漢果皂苷的酸水解及產物的純化 53 (六)靈芝對羅漢果皂苷的生物轉化 55 (七)附錄實驗 56 伍、結果與討論 58 一、Mogroside III E的製備與分離純化 58 (一)酵母菌對羅漢果皂苷的轉化 58 5.1.1.1 培養基及培養天數的確定 58 5.1.1.2 羅漢果萃取物的毒害性 62 5.1.1.3 500 mL擴大培養獲取mogroside III E 62 (二)HP-20樹脂分離純化mogroside III E 65 5.1.2.1 Mogroside III E檢量線 65 5.1.2.2 HP-20床體積測定 67 5.1.2.3 吸附等溫線(adsorption isotherm) 68 5.1.2.4 吸附動力學(adsorption kinetics) 71 5.1.2.5 pH值的影響 74 5.1.2.6 進樣速度及進樣體積測定 77 5.1.2.7 洗脫劑的選擇 79 5.1.2.7 洗脫劑的用量 81 5.1.2.8 HP-20管柱純化mogroside III E效果 89 二、Mogrol的製備及純化 91 (一)酸水解條件的確定 91 5.2.1.1 水解時長 91 5.2.1.2 放大實驗 94 (二)分液萃取純化mogrol 98 5.2.2.1 Mogrol檢量線 98 5.2.2.2 有機溶劑的選擇 99 5.2.2.3 有機溶劑的用量 100 三、靈芝對羅漢果皂苷的生物轉化 102 陸、結論與展望 104 柒、參考文獻 106 捌、附錄 115 | |
dc.language.iso | zh-TW | |
dc.title | 羅漢果皂苷的製備及純化 | zh_TW |
dc.title | Preparation and purification of mogrosides from Siraitia grosvenorii | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 謝淑貞(Shu-Chen Hsieh) | |
dc.contributor.oralexamcommittee | 呂廷璋(Ting-Jang Lu),陳宏彰(Hong-Jhang Chen),陳勁初(Chin-chu Chen,) | |
dc.subject.keyword | 羅漢果皂?,生物轉化,酸水解,HP-20樹脂,乙酸乙酯-水分液萃取, | zh_TW |
dc.subject.keyword | Siraitia grosvenorii,mogroside,biotransformation,acid hydrolysis,HP-20 resin,EA- H2O partition, | en |
dc.relation.page | 123 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-02-13 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
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