請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50822
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 游若? | |
dc.contributor.author | Jia-Hui Lu | en |
dc.contributor.author | 呂家慧 | zh_TW |
dc.date.accessioned | 2021-06-15T13:00:27Z | - |
dc.date.available | 2026-12-31 | |
dc.date.copyright | 2016-07-26 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-07-12 | |
dc.identifier.citation | 劉玉梅, 湯堅, 劉奎鈁, 啤酒花的化學研究及其和啤酒釀造的關係. 釀酒科技 2006, 71-75.
李寵臻, 王傳, 蔣寶航, 楊超, 孫珍, 堵國成, 啤酒花抗性機制的研究進展. 微生物學雜志 2015, 35, 1-7. 胡文姝, 啤酒與啤酒花化學. 國立中正大學研究所碩士論文 2003. 胡叔平, 啤酒花儲藏過程中苦味成份的變化. 江蘇食品與發酵 1982, 3, 013. 郭亮瑜, 評估啤酒花萃取物之抗菌性與及抗發炎性. 臺灣大學食品科技研究所碩士論文 2015, 1-83. 陳建男, 啤酒花抗氧化成分之研究. 大同大學生物工程研究所碩士論文2007. 楊宜蓁, 啤酒花萃取物對人體皮膚細胞抗光老化之評估. 臺灣大學食品科技研究所碩士論文 2015, 1-97. 趙昭明, 還我一張潔淨的臉. 大樹林出版社, 2004. Behr, J.; Vogel, R. F., Mechanisms of hop inhibition: hop ionophores. Journal of agricultural and food chemistry 2009, 57, 6074-6081. Blanco, C. A.; Rojas, A.; Caballero, P. A.; Ronda, F.; Gomez, M.; Caballero, I., A better control of beer properties by predicting acidity of hop iso-α-acids. Trends in food science & technology 2006, 17, 373-377. Bojar, R. A.; Holland, K. T., Acne and Propionibacterium acnes. Clinics in dermatology 2004, 22, 375-379. Bouwstra, J.; Honeywell-Nguyen, P., Skin structure and mode of action of vesicles. Advanced drug delivery reviews 2002, 54, S41-S55. Chen, W.-J.; Lin, J.-K., Mechanisms of cancer chemoprevention by hop bitter acids (beer aroma) through induction of apoptosis mediated by Fas and caspase cascades. Journal of agricultural and food chemistry 2004, 52, 55-64. Chen, W.; Becker, T.; Qian, F.; Ring, J., Beer and beer compounds: Physiological effects on skin health. Journal of the European academy of dermatology and venereology 2014, 28, 142-150. Cumberbatch, M.; Dearman, R. J.; Griffiths, C. E.; Kimber, I., Epidermal Langerhans cell migration and sensitisation to chemical allergens. APMIS 2003, 111, 797-804. De Keukeleire, D., Fundamentals of beer and hop chemistry. Quimica nova 2000, 23, 108-112. De Keukeleire, J.; Ooms, G.; Heyerick, A.; Roldan-Ruiz, I.; Van Bockstaele, E.; De Keukeleire, D., Formation and accumulation of α-acids, β-acids, desmethylxanthohumol, and xanthohumol during flowering of hops (Humulus lupulus L.). Journal of agricultural and food chemistry 2003, 51, 4436-4441. del Valle, J. M.; Rivera, O.; Teuber, O.; Palma, M. T., Supercritical CO2 extraction of Chilean hop (Humulus lupulus) ecotypes. Journal of the science of food and agriculture 2003, 83, 1349-1356. Deplewski, D.; Rosenfield, R. L., Role of hormones in pilosebaceous unit development. Endocrine reviews 2000, 21, 363-392. Eady, E.; Gloor, M.; Leyden, J., Propionibacterium acnes resistance: a worldwide problem. Dermatology 2003, 206, 54-56. Egts, H.; Durben, D. J.; Dixson, J. A.; Zehfus, M. H., A multicomponent UV analysis of α-and β-acids in hops. Journal of chemical education 2011, 89, 117-120. Elman, M.; Lebzelter, J., Light therapy in the treatment of acne vulgaris. Dermatologic surgery 2004, 30, 139-146. Gagnon, D.; Wendakoon, C.; Smith, R.; Leker, J., Stability of active constituents of hops (Humulus lupulus) strobiles and their ethanolic extracts during storage. European journal of medicinal plants 2014, 4, 1302-1312. García-Villalba, R.; Cortacero-Ramírez, S.; Segura-Carretero, A.; Martín-Lagos Contreras, J. A.; Fernández-Gutiérrez, A., Analysis of hop acids and their oxidized derivatives and iso-α-acids in beer by capillary electrophoresis-electrospray ionization mass spectrometry. Journal of agricultural and food chemistry 2006, 54, 5400-5409. Gerhauser, C.; Alt, A.; Heiss, E.; Gamal-Eldeen, A.; Klimo, K.; Knauft, J.; Neumann, I.; Scherf, H.-R.; Frank, N.; Bartsch, H., Cancer Chemopreventive Activity of Xanthohumol, a Natural Product Derived from Hop 1 Support for this work has been provided by Verein zur Förderung der Krebsforschung in Deutschland eV and by Wissenschaftsförderung der Deutschen Brauwirtschaft eV These data were presented, in part, at the 92nd annual meeting of the American Association of Cancer Research, March 24–28, 2001 in New Orleans, LA (64). 1. Molecular cancer therapeutics 2002, 1, 959-969. Gollnick, H.; Cunliffe, W.; Berson, D.; Dreno, B.; Finlay, A.; Leyden, J. J.; Shalita, A. R.; Thiboutot, D., Management of acne: A report from a global alliance to improve outcomes in acne. Journal of the american academy of dermatology 2003, 49, S1-S37. Haake, A.; Scott, G. A.; Holbrook, K. A., Structure and function of the skin: Overview of the epidermis and dermis. The biology of the skin 2001, 19-45. Haas, G. J.; Barsoumian, R., Antimicrobial activity of hop resins. Journal of food protection 1994, 57, 59-61. Haider, A.; Shaw, J. C., Treatment of acne vulgaris. JAMA 2004, 292, 726-735. Ingham, E.; Eady, E. A.; Goodwin, C. E.; Cove, J. H.; Cunliffe, W. J., Pro-inflammatory levels of interleukin-1 alpha-Like bioactivity are present in the majority of open comedones in acne vulgaris. Journal of investigative dermatology 1992, 98, 895-901. Jappe U., Pathological mechanisms of acne with special emphasis on Propionibacterium acnes and related therapy. Acta Derm Venereol 2003, 83, 241-248. Jugeau, S.; Tenaud, I.; Knol, A.; Jarrousse, V.; Quereux, G.; Khammari, A.; Dreno, B., Induction of toll‐like receptors by Propionibacterium acnes. British Journal of Dermatology 2005, 153, 1105-1113. Kim, J., Review of the innate immune response in acne vulgaris: Activation of Toll-like receptor 2 in acne triggers inflammatory cytokine responses. Dermatology 2005, 211, 193-198. Knaggs, H. E.; Holland, D. B.; Morris, C.; Wood, E. J.; Cunliffe, W. J., Quantification of cellular proliferation in acne using the monoclonal antibody Ki-67. Journal of investigative dermatology 1994, 102, 89-92. Kraft, J.; Freiman, A., Management of acne. Canadian Medical Association Journal 2011, 183, E430-E435. Krautheim, A.; Gollnick, H. P., Acne: Topical treatment. Clinics in dermatology 2004, 22, 398-407. Krofta, K.; Mikyška, A.; Hašková, D., Antioxidant characteristics of hops and hop products. Journal of the institute of brewing 2008, 114, 160-166. Moir, M., Hops: A millennium review. Journal of the American Society of Brewing Chemists 2000, 58, 131-146. Molyneux, P., The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin journal of science and technology 2004, 26, 211-219. Nickerson, G. B.; Likens, S. T. Hop storage index. Journal of the american society of brewing chemists 1979, 37, 36-24. Natarajan, P.; Katta, S.; Andrei, I.; Ambati, V. B. R.; Leonida, M.; Haas, G., Positive antibacterial co-action between hop (Humulus lupulus) constituents and selected antibiotics. Phytomedicine 2008, 15, 194-201. Negrão, R.; Costa, R.; Duarte, D.; Gomes, T. T.; Coelho, P.; Guimarães, J. T.; Guardão, L.; Azevedo, I.; Soares, R., Xanthohumol‐supplemented beer modulates angiogenesis and inflammation in a skin wound healing model. Involvement of local adipocytes. Journal of cellular biochemistry 2012, 113, 100-109. Payet, B.; Shum Cheong Sing, A.; Smadja, J., Assessment of antioxidant activity of cane brown sugars by ABTS and DPPH radical scavenging assays: determination of their polyphenolic and volatile constituents. Journal of agricultural and food chemistry 2005, 53, 10074-10079. Proksch, E.; Brandner, J. M.; Jensen, J. M., The skin: An indispensable barrier. Experimental dermatology 2008, 17, 1063-1072. Rückle, L.; Senn, T., Hop acids as natural antibacterials can efficiently replace antibiotics in ethanol production. BetaTec Hopfenprodukte GmbH, Freiligrathstr 2006, 108, 139 -147. Ross, J.; Snelling, A.; Carnegie, E.; Coates, P.; Cunliffe, W.; Bettoli, V.; Tosti, G.; Katsambas, A.; Galvan Perez Del Pulgar, J.; Rollman, O., Antibiotic‐resistant acne: lessons from Europe. British journal of dermatology 2003, 148, 467-478. Rossbauer, G.; Münsterer, J. Optimisation of hop drying and conditioning for the safeguarding of hop quality, Proc. of IHGC Technical Commission Meeting. Sofia,(http://www. czhops. cz/tc), 2003. Schmalreck, A. F.; Teuber, M.; Reininger, W.; Hartl, A., Structural features determining the antibiotic potencies of natural and synthetic hop bitter resins, their precursors and derivatives. Canadian journal of microbiology 1975, 21, 205-212. Schurr, B. C.; Hahne, H.; Kuster, B.; Behr, J.; Vogel, R. F., Molecular mechanisms behind the antimicrobial activity of hop iso-α-acids in Lactobacillus brevis. Food microbiology 2015, 46, 553-563. Shai, A.; Maibach, H. I.; Baran, R., Handbook of cosmetic skin care. Martin Dunitz, London, 2001. Sharma, R.; Lall, N., Antibacterial, antioxidant activities and cytotoxicity of plants against Propionibacterium acnes. South African Journal of Science 2014, 110, 01-08. Shimamura, M.; Hazato, T.; Ashino, H.; Yamamoto, Y.; Iwasaki, E.; Tobe, H.; Yamamoto, K.; Yamamoto, S., Inhibition of angiogenesis by humulone, a bitter acid from beer hop. Biochemical and biophysical research communications 2001, 289, 220-224. Siegel, L.; Miternique-Grosse, A.; Griffon, C.; Klein-Soyer, C.; Lobstein, A.; Raul, F.; Stephan, D., Antiangiogenic properties of lupulone, a bitter acid of hop cones. Anticancer research 2008, 28, 289-294. Simpson, W.; Smith, A., Factors affecting antibacterial activity of hop compounds and their derivatives. Journal of applied bacteriology 1992, 72, 327-334. Soobrattee, M. A.; Neergheen, V. S.; Luximon-Ramma, A.; Aruoma, O. I.; Bahorun, T., Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutation research/fundamental and molecular mechanisms of mutagenesis 2005, 579, 200-213. Spetsig, L.; Steninger, M., Hulupones, a new group of hop bitter substances. Journal of the institute of brewing 1960, 66, 413-417. Stevens, J. F.; Ivancic, M.; Hsu, V. L.; Deinzer, M. L., Prenylflavonoids from Humulus lupulus. Phytochemistry 1997, 44, 1575-1585. Stevens, J. F.; Taylor, A. W.; Clawson, J. E.; Deinzer, M. L., Fate of xanthohumol and related prenylflavonoids from hops to beer. Journal of agricultural and food chemistry 1999, 47, 2421-2428. Stevens, J. F.; Page, J. E., Xanthohumol and related prenylflavonoids from hops and beer: To your good health! Phytochemistry 2004, 65, 1317-1330. Stevens, R.; Wright, D., Evaluation of hops. x. hulupones and the significance of β acids in brewing. Journal of the institute of brewing 1961, 67, 496-501. Tagasgira, M.; Watanabe, M.; Uemitsu, N., Antioxidative activity of hop bitter acids and their analogues. Bioscience, biotechnology, and biochemistry 1995, 59, 740-742. Taniguchi, Y.; Taniguchi, H.; Matsukura, Y.; Kawachi, Y.; Shindo, K., Structural elucidation of humulone autoxidation products and analysis of their occurrence in stored hops. Journal of natural products 2014, 77, 1252-1261. Taylor, A. W.; Barofsky, E.; Kennedy, J. A.; Deinzer, M. L., Hop (Humulus lupulus L.) proanthocyanidins characterized by mass spectrometry, acid catalysis, and gel permeation chromatography. Journal of agricultural and food chemistry 2003, 51, 4101-4110. Thiboutot, D. M.; Strauss, J. S., Diet and acne revisited. Archives of dermatology 2002, 138, 1591-1592. Toyoda, M.; Morohashi, M., Pathogenesis of acne. Medical Electron Microscopy 2001, 34, 29-40. Tsai, H.-H.; Lee, W.-R.; Wang, P.-H.; Cheng, K.-T.; Chen, Y.-C.; Shen, S.-C., Propionibacterium acnes - induced iNOS and COX-2 protein expression via ROS-dependent NF-κB and AP-1 activation in macrophages. Journal of dermatological science 2013, 69, 122-131. Van Cleemput, M.; Cattoor, K.; De Bosscher, K.; Haegeman, G.; De Keukeleire, D.; Heyerick, A., Hop (Humulus lupulus) -derived bitter acids as multipotent bioactive compounds. Journal of natural products 2009, 72, 1220-1230. Verzele, M.; De Keukeleire, D., Chemistry and analysis of hop and beer bitter acids. Elsevier, Amsterdam, 1991 Vowels, B. R.; Yang, S.; Leyden, J. J., Induction of proinflammatory cytokines by a soluble factor of Propionibacterium acnes: Implications for chronic inflammatory acne. Infection and immunity 1995, 63, 3158-3165. White, S. D.; Yager, J. A., Resident dendritic cells in the epidermis: Langerhans cells, Merkel cells and melanocytes. Veterinary dermatology 1995, 6, 1-8. Worret, W. I.; Fluhr, J. W., Acne therapy with topical benzoyl peroxide, antibiotics and azelaic acid. JDDG: Journal der Deutschen Dermatologischen Gesellschaft 2006, 4, 293-300. Yamaguchi, N.; Satoh-Yamaguchi, K.; Ono, M., In vitro evaluation of antibacterial, anticollagenase, and antioxidant activities of hop components (Humulus lupulus) addressing acne vulgaris. Phytomedicine 2009, 16, 369-376. Zanoli, P.; Zavatti, M., Pharmacognostic and pharmacological profile of Humulus lupulus L. Journal of Ethnopharmacology 2008, 116, 383-396. Zhao, F.; Nozawa, H.; Daikonnya, A.; Kondo, K.; Kitanaka, S., Inhibitors of nitric oxide production from hops (Humulus lupulus L.). Biological and Pharmaceutical Bulletin 2003, 26, 61-65. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/50822 | - |
dc.description.abstract | 啤酒花廣泛用於啤酒釀造過程中賦予啤酒苦味以及特殊香氣。研究指出啤酒花中苦味酸化合物葎草酮 (humulone)、蛇麻酮 (lupulone),以及多酚類化合物等活性成分,具有抗菌、抗發炎、抗氧化、抑制癌細胞增生等功效。尋常性痤瘡 (acnevulgaris) 俗稱青春痘,是一種常見的皮膚疾病,而痤瘡桿菌 (Propionibacterium acnes, P. acnes) 對尋常性痤瘡形成扮演著相當重要的角色。啤酒花中活性成分的穩定性相當重要,但這些成分易受光、溫度、氧氣的影響而劣化。為促使啤酒花能獲得更廣泛之應用,故本研究欲探討以不同酒精濃度 (55%、75%、95%) 萃取之啤酒花萃取物 (hops ethanol extracts,HEE),及以超臨界流體萃取之啤酒花浸膏對痤瘡桿菌之抑菌效果,並探討啤酒花萃取物在儲存期間之抗痤瘡桿菌與抗氧化活性的變化,萃取物分別儲存於室溫 (25°C) 及冷藏 (4°C) 環境下 3 個月,藉此評估啤酒花應用在皮膚保健產品上的潛力。研究結果顯示,不同啤酒花萃取物對痤瘡桿菌皆有良好抑菌及殺菌效果,且以啤酒花浸膏殺菌效果最佳,抑制 P. acnes 生長的最小抑制濃度 (MIC) 和最小殺菌濃度 (MBC) 為 2 μg/ml 和 4 μg/ml。而儲存試驗結果顯示,經過 3 個月儲藏後,95%酒精萃取之啤酒花萃取物及啤酒花浸膏之抗菌及抗氧化活性較穩定,抗氧化力分別下降 0.06%、15.28%,而 55%酒精萃取之啤酒花萃取物,抗氧化力下降 38.43%,其抗菌及抗氧化活性有明顯下降的現象 (P < 0.05),透過低溫儲存可減緩其下降趨勢。因此,未來欲利用啤酒花進行產品開發,可選擇較安定的啤酒花浸膏或 95%啤酒花酒精萃取物來進行,尚需注意其儲存環境,以確保其效用。 | zh_TW |
dc.description.abstract | Hops, the female inflorescences of the hop plant (Humulus lupulus L.), are widely used in the brewing industry to add bitterness and aroma to beer. Hops have attracted a great deal of attention as a source of small molecules such as humulone, lupulone and xanthohumol with potential for beneficial effects on antibacterial,antiinflammatory, antioxidation and antiproliferation. Propionibacterium acnes (P. acnes) play important roles in common skin disease of acne vulgaris. The stability of the constituents of hops has been great important. However, hop constituents are quite unstable and sensitive to heat, light, and oxygen. In addition, oxidation and degradation occurring during the storage period reduce the active ingredients content and antibacterial activity. In order to increase application of hops, the aim of this work is to evaluate antibacterial activity of the hops extracts obtained under various extract conditions, and their antibacterial and antioxidant activity changes during storage period. Thus, in this work, hops extracts made with various ethanol concentrations (55%, 75%, 95%) and CO2 hops extract were stored at different temperatures (4°C / 25°C) for three months to study their antibacterial activity against P. acnes and antioxidant activity changes during storage period. The results showed that CO2 hops extract and 95% hops ethanol extract exhibited higher antibacterial and antioxidant activities than other hops extracts and remained stable after three months storage. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of CO2 hops extract was 2 μg/ml and 4 μg/ml, respectively. The 55% hops ethanol extract showed a significant decrease in antibacterial and antioxidant activities after storage, but the activities can be improved by storage at a lower temperature. These results suggested that the CO2 hops extract and 95% hops ethanol extract are more suitable for skin care products and the storage environment should be noticed to ensure quality of the extracts. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T13:00:27Z (GMT). No. of bitstreams: 1 ntu-105-R03641015-1.pdf: 1877609 bytes, checksum: f4ebe9604139cf22ae4f4d7342c6c95e (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 誌謝………………………………………………………………………………I
中文摘要………………………………………………………………… III 英文摘要………………………………………………………………… IV 目錄…………………………………………………………………………… V 圖次……………………………………………………………………………VII 表次………………………………………………………………………… VIII 壹、前言………………………………………………………………1 貳、文獻回顧………………………………………………………………3 一、啤酒花的歷史和應用……………………………3 二、皮膚結構………………………………………11 三、痤瘡的致病機制……………………………………14 四、痤瘡的治療……………………17 參、實驗目的與架構………………………………………………21 一、實驗目的………………………………………………….21 二、實驗架構………………………………22 肆、材料與方法……………………………………………24 一、實驗材料……………………………………………24 二、實驗儀器、設備與耗材……………………………………25 三、實驗方法……………………………………………27 (一) 樣品製備與保存………………………………27 (二) 微生物培養、活化與保存……………………27 (三) 總多酚測定………………………………………28 (四) 微生物抑菌試驗…………………………………………28 VI (五) 苦味酸含量測定………………………………29 (六) 清除DPPH自由基能力測定……………30 (七) 儲存安定性實驗…………………………………30 (八) 統計與分析…………………………………31 伍、結果與討論……………………………………………32 一、啤酒花酒精萃取物製備與收率…………………………….32 二、啤酒花萃取物對痤瘡桿菌抗菌活性……………………34 三、啤酒花萃取物苦味酸含量………………………………………37 四、儲存期間啤酒花萃取物對痤瘡桿菌抗菌活性之變化………………39 五、儲存期間啤酒花萃取物抗氧化活性之變化………………42 六、儲存期間啤酒花萃取物總多酚類化合物含量變化…………………51 陸、結論………………………………………………………59 柒、參考文獻……………………………………………………60 捌、附錄……………………………………………………68 | |
dc.language.iso | zh-TW | |
dc.title | 儲存期間啤酒花萃取物之抗氧化及對痤瘡桿菌抗菌之影響 | zh_TW |
dc.title | Effect of storage on the antioxidative and antibacterial effect of hops extracts against Propionibacterium acnes | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 鄭光成 | |
dc.contributor.oralexamcommittee | 林俊杰(jiun@mail.ttl.com.tw),潘崇良,周正俊 | |
dc.subject.keyword | 啤酒花萃取物,抗菌,抗氧化,痤瘡桿菌, | zh_TW |
dc.subject.keyword | Humulus lupulus,antibacterial,antioxidation,Propionibacterium acnes, | en |
dc.relation.page | 68 | |
dc.identifier.doi | 10.6342/NTU201600820 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-07-12 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 食品科技研究所 | zh_TW |
顯示於系所單位: | 食品科技研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-105-1.pdf 目前未授權公開取用 | 1.83 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。